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Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)
Wu, Shangong1; Tian, Jingyun2; Wang, Guitang1; Li, Wenxiang1; Zou, Hong1; Wang, GT (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
2012-05-01
Source PublicationWORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN0959-3993
Volume28Issue:5Pages:2165-2174
AbstractIn this study, the traditional culture-based technique and the 16S rDNA sequencing method were used to investigate the characterization of bacterial community in the stomach contents and mucus of yellow catfish (Pelteobagrus fulvidraco). The culture-based technique disclosed that the average bacterial numbers in the gastric contents and mucus were 5.79 x 10(7) cfu/g (cfu: colony forming unit) and 1.89 x 10(5) cfu/g, respectively. Several different bacteria were obtained from gastric contents, including species from genera Bradyrhizobium, Phyllobacterium, Plesiomonas, Hafnia, Edwardsiella, Pseudomonas, and Bacillus. However, only two species were isolated from the gastric mucus, including species from genera Plesiomonas and Aeromonas. Forty-five phylotypes were observed from the 65 positive clones from the stomach contents (library SC); nineteen phylotypes were detected from the 45 clones from the stomach mucus (library SM). Further analyses revealed that the fish stomach harbored characteristic microbiota, where Firmicutes was dominant, followed by Proteobacteria and Bacteroidetes and Fusobacteria. This characterization of bacterial community is markedly different from that of the fish intestine, where Proteobacteria is predominant, followed by Fusobacteria and Firmicutes. Chloroflexi (1.5%) was only found in the library SC, while Actinobacteria (4.4%) was only found in the library SM, suggesting that microbiota of GI contents was quite different from that of GI mucus. In addition, several species of bacteria found in the stomach may be potentially opportunistic pathogens, indicating that fish digestive tract is a reservoir for many nosocomial pathogens.; In this study, the traditional culture-based technique and the 16S rDNA sequencing method were used to investigate the characterization of bacterial community in the stomach contents and mucus of yellow catfish (Pelteobagrus fulvidraco). The culture-based technique disclosed that the average bacterial numbers in the gastric contents and mucus were 5.79 x 10(7) cfu/g (cfu: colony forming unit) and 1.89 x 10(5) cfu/g, respectively. Several different bacteria were obtained from gastric contents, including species from genera Bradyrhizobium, Phyllobacterium, Plesiomonas, Hafnia, Edwardsiella, Pseudomonas, and Bacillus. However, only two species were isolated from the gastric mucus, including species from genera Plesiomonas and Aeromonas. Forty-five phylotypes were observed from the 65 positive clones from the stomach contents (library SC); nineteen phylotypes were detected from the 45 clones from the stomach mucus (library SM). Further analyses revealed that the fish stomach harbored characteristic microbiota, where Firmicutes was dominant, followed by Proteobacteria and Bacteroidetes and Fusobacteria. This characterization of bacterial community is markedly different from that of the fish intestine, where Proteobacteria is predominant, followed by Fusobacteria and Firmicutes. Chloroflexi (1.5%) was only found in the library SC, while Actinobacteria (4.4%) was only found in the library SM, suggesting that microbiota of GI contents was quite different from that of GI mucus. In addition, several species of bacteria found in the stomach may be potentially opportunistic pathogens, indicating that fish digestive tract is a reservoir for many nosocomial pathogens.
SubtypeArticle
KeywordMicrobiota Pelteobagrus Fulvidraco 16s Rdna Clone Library Stomach
Department[Wu, Shangong; Wang, Guitang; Li, Wenxiang; Zou, Hong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Tian, Jingyun] Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China
DOI10.1007/s11274-012-1022-5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08]
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000303385900033
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; INTESTINAL MICROFLORA ; MICROBIAL DIVERSITY ; FISH ; TRACT ; SEQUENCES ; MUCUS ; CAGES ; L.
Funding OrganizationNational Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08]
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16939
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, GT (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Wu, Shangong,Tian, Jingyun,Wang, Guitang,et al. Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2012,28(5):2165-2174.
APA Wu, Shangong,Tian, Jingyun,Wang, Guitang,Li, Wenxiang,Zou, Hong,&Wang, GT .(2012).Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco).WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,28(5),2165-2174.
MLA Wu, Shangong,et al."Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco)".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 28.5(2012):2165-2174.
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