IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus
Chen, Zhong-Yuan; Lei, Xiao-Ying; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2012-06-15
Source PublicationVETERINARY MICROBIOLOGY
ISSN0378-1135
Volume157Issue:3-4Pages:264-275
AbstractPlasmid DNAs containing Siniperca chuatsi rhabdovirus (SCRV) glycoprotein gene (pcDNA-G) and nucleoprotein gene (pcDNA-N) were constructed, and used to determine the antiviral immune response elicited by DNA vaccination in mandarin fish. In vitro and in vivo expression of the plasmid constructs was confirmed in transfected cells and muscle tissues of vaccinated fish by Western blot, indirect immunofluorescence or RT-PCR analysis. Fish injected with pcDNA-G exhibited protective effect against SCRV challenge with a relative percent survival (RPS) of 77.5%, but no significant protection (RPS of 2.5%) was observed in fish vaccinated with pcDNA-N. Immunohistochemical analysis showed that vaccination with pcDNA-G decreased histological lesions and suppressed the virus replication in fish target organs, e.g. kidney, liver, spleen, gill and heart. Transcriptional analysis further revealed that the expression levels of type I IFN system genes including interferon regulation factor-7 (IRF-7) gene, myxovirus resistance (Mx) gene and virus inhibitory protein (Viperin) gene were strongly up-regulated after injection with pcDNA-G, whereas the level of transcription of immunoglobulin M (IgM) gene did not show a statistically significant change. These results reveal that type I IFN antiviral immune response is rapidly triggered by the plasmid DNA containing rhabdovirus glycoprotein gene in fish, which offers an explanation of molecular mechanisms for DNA vaccination inducing mandarin fish resist to SCRV disease. (C) 2011 Elsevier B.V. All rights reserved.; Plasmid DNAs containing Siniperca chuatsi rhabdovirus (SCRV) glycoprotein gene (pcDNA-G) and nucleoprotein gene (pcDNA-N) were constructed, and used to determine the antiviral immune response elicited by DNA vaccination in mandarin fish. In vitro and in vivo expression of the plasmid constructs was confirmed in transfected cells and muscle tissues of vaccinated fish by Western blot, indirect immunofluorescence or RT-PCR analysis. Fish injected with pcDNA-G exhibited protective effect against SCRV challenge with a relative percent survival (RPS) of 77.5%, but no significant protection (RPS of 2.5%) was observed in fish vaccinated with pcDNA-N. Immunohistochemical analysis showed that vaccination with pcDNA-G decreased histological lesions and suppressed the virus replication in fish target organs, e.g. kidney, liver, spleen, gill and heart. Transcriptional analysis further revealed that the expression levels of type I IFN system genes including interferon regulation factor-7 (IRF-7) gene, myxovirus resistance (Mx) gene and virus inhibitory protein (Viperin) gene were strongly up-regulated after injection with pcDNA-G, whereas the level of transcription of immunoglobulin M (IgM) gene did not show a statistically significant change. These results reveal that type I IFN antiviral immune response is rapidly triggered by the plasmid DNA containing rhabdovirus glycoprotein gene in fish, which offers an explanation of molecular mechanisms for DNA vaccination inducing mandarin fish resist to SCRV disease. (C) 2011 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMandarin Fish Rhabdovirus Dna Vaccination Antiviral Defense Mechanisms
Department[Chen, Zhong-Yuan; Lei, Xiao-Ying; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.vetmic.2011.12.025
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program[2009CB118704, 2010CB126303]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ16]; Chinese Academy of Sciences[2010113] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ16]; Chinese Academy of Sciences[2010113] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ16]; Chinese Academy of Sciences[2010113] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ16]; Chinese Academy of Sciences[2010113]
Indexed BySCI
Language英语
WOS Research AreaMicrobiology ; Veterinary Sciences
WOS SubjectMicrobiology ; Veterinary Sciences
WOS IDWOS:000304643800002
WOS KeywordHEMATOPOIETIC NECROSIS VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; PARALICHTHYS-OLIVACEUS ; SPRING VIREMIA ; TIME-COURSE ; CARP VIRUS ; VACCINES ; GENE ; EXPRESSION
Funding OrganizationNational Major Basic Research Program[2009CB118704, 2010CB126303]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ16]; Chinese Academy of Sciences[2010113] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ16]; Chinese Academy of Sciences[2010113] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ16]; Chinese Academy of Sciences[2010113] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ16]; Chinese Academy of Sciences[2010113]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16929
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen, Zhong-Yuan,Lei, Xiao-Ying,Zhang, Qi-Ya,et al. The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus[J]. VETERINARY MICROBIOLOGY,2012,157(3-4):264-275.
APA Chen, Zhong-Yuan,Lei, Xiao-Ying,Zhang, Qi-Ya,&Zhang, QY .(2012).The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus.VETERINARY MICROBIOLOGY,157(3-4),264-275.
MLA Chen, Zhong-Yuan,et al."The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus".VETERINARY MICROBIOLOGY 157.3-4(2012):264-275.
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