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题名: The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus
作者: Chen, Zhong-Yuan1; Lei, Xiao-Ying1; Zhang, Qi-Ya1
通讯作者: Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: Mandarin fish ; Rhabdovirus ; DNA vaccination ; Antiviral defense mechanisms
刊名: VETERINARY MICROBIOLOGY
发表日期: 2012-06-15
DOI: 10.1016/j.vetmic.2011.12.025
卷: 157, 期:3-4, 页:264-275
收录类别: SCI
文章类型: Article
部门归属: [Chen, Zhong-Yuan; Lei, Xiao-Ying; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program[2009CB118704, 2010CB126303]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ16]; Chinese Academy of Sciences[2010113]
类目[WOS]: Microbiology ; Veterinary Sciences
研究领域[WOS]: Microbiology ; Veterinary Sciences
摘要: Plasmid DNAs containing Siniperca chuatsi rhabdovirus (SCRV) glycoprotein gene (pcDNA-G) and nucleoprotein gene (pcDNA-N) were constructed, and used to determine the antiviral immune response elicited by DNA vaccination in mandarin fish. In vitro and in vivo expression of the plasmid constructs was confirmed in transfected cells and muscle tissues of vaccinated fish by Western blot, indirect immunofluorescence or RT-PCR analysis. Fish injected with pcDNA-G exhibited protective effect against SCRV challenge with a relative percent survival (RPS) of 77.5%, but no significant protection (RPS of 2.5%) was observed in fish vaccinated with pcDNA-N. Immunohistochemical analysis showed that vaccination with pcDNA-G decreased histological lesions and suppressed the virus replication in fish target organs, e.g. kidney, liver, spleen, gill and heart. Transcriptional analysis further revealed that the expression levels of type I IFN system genes including interferon regulation factor-7 (IRF-7) gene, myxovirus resistance (Mx) gene and virus inhibitory protein (Viperin) gene were strongly up-regulated after injection with pcDNA-G, whereas the level of transcription of immunoglobulin M (IgM) gene did not show a statistically significant change. These results reveal that type I IFN antiviral immune response is rapidly triggered by the plasmid DNA containing rhabdovirus glycoprotein gene in fish, which offers an explanation of molecular mechanisms for DNA vaccination inducing mandarin fish resist to SCRV disease. (C) 2011 Elsevier B.V. All rights reserved.
英文摘要: Plasmid DNAs containing Siniperca chuatsi rhabdovirus (SCRV) glycoprotein gene (pcDNA-G) and nucleoprotein gene (pcDNA-N) were constructed, and used to determine the antiviral immune response elicited by DNA vaccination in mandarin fish. In vitro and in vivo expression of the plasmid constructs was confirmed in transfected cells and muscle tissues of vaccinated fish by Western blot, indirect immunofluorescence or RT-PCR analysis. Fish injected with pcDNA-G exhibited protective effect against SCRV challenge with a relative percent survival (RPS) of 77.5%, but no significant protection (RPS of 2.5%) was observed in fish vaccinated with pcDNA-N. Immunohistochemical analysis showed that vaccination with pcDNA-G decreased histological lesions and suppressed the virus replication in fish target organs, e.g. kidney, liver, spleen, gill and heart. Transcriptional analysis further revealed that the expression levels of type I IFN system genes including interferon regulation factor-7 (IRF-7) gene, myxovirus resistance (Mx) gene and virus inhibitory protein (Viperin) gene were strongly up-regulated after injection with pcDNA-G, whereas the level of transcription of immunoglobulin M (IgM) gene did not show a statistically significant change. These results reveal that type I IFN antiviral immune response is rapidly triggered by the plasmid DNA containing rhabdovirus glycoprotein gene in fish, which offers an explanation of molecular mechanisms for DNA vaccination inducing mandarin fish resist to SCRV disease. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: HEMATOPOIETIC NECROSIS VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; PARALICHTHYS-OLIVACEUS ; SPRING VIREMIA ; TIME-COURSE ; CARP VIRUS ; VACCINES ; GENE ; EXPRESSION
语种: 英语
WOS记录号: WOS:000304643800002
ISSN号: 0378-1135
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16929
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Chen, Zhong-Yuan; Lei, Xiao-Ying; Zhang, Qi-Ya.The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus,VETERINARY MICROBIOLOGY,2012,157(3):264-275
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