IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line
Zhou, Guang-Zhou1,2; Zhu, Rong1; Gui, Jian-Fang1; Zhang, Qi-Ya1; Zhou, GZ (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2012-03-01
Source PublicationFISH PATHOLOGY
ISSN0388-788X
Volume47Issue:1Pages:30-32
AbstractWe evaluated the antiviral activity of several small interfering RNAs (siRNAs) corresponding to the Siniperca chuatsi rhabdovirus (SCRV) nucleoprotein (N) gene against the SCRV in a fish cell line, EPC. Among the siRNAs we examined, si983 was most effective at reducing virus titer compared to the mock group. Western blot analysis suggested that si983 inhibited N gene expression and virus replication in a dose-dependent manner. These results indicate that N gene 983 sites are potential targets for antiviral therapy. Furthermore, RNAi targeting of the N gene may represent a viable method of treating against SCRV infection.; We evaluated the antiviral activity of several small interfering RNAs (siRNAs) corresponding to the Siniperca chuatsi rhabdovirus (SCRV) nucleoprotein (N) gene against the SCRV in a fish cell line, EPC. Among the siRNAs we examined, si983 was most effective at reducing virus titer compared to the mock group. Western blot analysis suggested that si983 inhibited N gene expression and virus replication in a dose-dependent manner. These results indicate that N gene 983 sites are potential targets for antiviral therapy. Furthermore, RNAi targeting of the N gene may represent a viable method of treating against SCRV infection.
SubtypeArticle
KeywordMandarin Fish Rhabdovirus Nucleoprotein Rna Interference Scrv
Department[Zhou, Guang-Zhou; Zhu, Rong; Gui, Jian-Fang; Zhang, Qi-Ya] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhou, Guang-Zhou] Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Veterinary Sciences
WOS SubjectFisheries ; Veterinary Sciences
WOS IDWOS:000305037500007
WOS KeywordMANDARIN FISH ; SEQUENCE
Funding OrganizationNational Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016]
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16927
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhou, GZ (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Guang-Zhou,Zhu, Rong,Gui, Jian-Fang,et al. Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line[J]. FISH PATHOLOGY,2012,47(1):30-32.
APA Zhou, Guang-Zhou,Zhu, Rong,Gui, Jian-Fang,Zhang, Qi-Ya,&Zhou, GZ .(2012).Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line.FISH PATHOLOGY,47(1),30-32.
MLA Zhou, Guang-Zhou,et al."Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line".FISH PATHOLOGY 47.1(2012):30-32.
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