| Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line | |
| Zhou, Guang-Zhou1,2; Zhu, Rong1; Gui, Jian-Fang1; Zhang, Qi-Ya1; Zhou, GZ (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China. | |
| 2012-03-01 | |
| Source Publication | FISH PATHOLOGY
 |
| ISSN | 0388-788X |
| Volume | 47Issue:1Pages:30-32 |
| Abstract | We evaluated the antiviral activity of several small interfering RNAs (siRNAs) corresponding to the Siniperca chuatsi rhabdovirus (SCRV) nucleoprotein (N) gene against the SCRV in a fish cell line, EPC. Among the siRNAs we examined, si983 was most effective at reducing virus titer compared to the mock group. Western blot analysis suggested that si983 inhibited N gene expression and virus replication in a dose-dependent manner. These results indicate that N gene 983 sites are potential targets for antiviral therapy. Furthermore, RNAi targeting of the N gene may represent a viable method of treating against SCRV infection.; We evaluated the antiviral activity of several small interfering RNAs (siRNAs) corresponding to the Siniperca chuatsi rhabdovirus (SCRV) nucleoprotein (N) gene against the SCRV in a fish cell line, EPC. Among the siRNAs we examined, si983 was most effective at reducing virus titer compared to the mock group. Western blot analysis suggested that si983 inhibited N gene expression and virus replication in a dose-dependent manner. These results indicate that N gene 983 sites are potential targets for antiviral therapy. Furthermore, RNAi targeting of the N gene may represent a viable method of treating against SCRV infection. |
| Subtype | Article |
| Keyword | Mandarin Fish Rhabdovirus Nucleoprotein Rna Interference Scrv |
| Department | [Zhou, Guang-Zhou; Zhu, Rong; Gui, Jian-Fang; Zhang, Qi-Ya] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhou, Guang-Zhou] Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Veterinary Sciences |
| WOS Subject | Fisheries ; Veterinary Sciences |
| WOS ID | WOS:000305037500007 |
| WOS Keyword | MANDARIN FISH ; SEQUENCE |
| Funding Organization | National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] ; National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/16927 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Zhou, GZ (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China |
| Recommended Citation GB/T 7714 | Zhou, Guang-Zhou,Zhu, Rong,Gui, Jian-Fang,et al. Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line[J]. FISH PATHOLOGY,2012,47(1):30-32. |
| APA | Zhou, Guang-Zhou,Zhu, Rong,Gui, Jian-Fang,Zhang, Qi-Ya,&Zhou, GZ .(2012).Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line.FISH PATHOLOGY,47(1),30-32. |
| MLA | Zhou, Guang-Zhou,et al."Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line".FISH PATHOLOGY 47.1(2012):30-32. |
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