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题名: Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line
作者: Zhou, Guang-Zhou1, 2; Zhu, Rong1; Gui, Jian-Fang1; Zhang, Qi-Ya1
通讯作者: Zhou, GZ (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Mandarin fish ; rhabdovirus ; nucleoprotein ; RNA interference ; SCRV
刊名: FISH PATHOLOGY
发表日期: 2012-03-01
卷: 47, 期:1, 页:30-32
收录类别: SCI
文章类型: Article
部门归属: [Zhou, Guang-Zhou; Zhu, Rong; Gui, Jian-Fang; Zhang, Qi-Ya] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhou, Guang-Zhou] Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016]
类目[WOS]: Fisheries ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Veterinary Sciences
摘要: We evaluated the antiviral activity of several small interfering RNAs (siRNAs) corresponding to the Siniperca chuatsi rhabdovirus (SCRV) nucleoprotein (N) gene against the SCRV in a fish cell line, EPC. Among the siRNAs we examined, si983 was most effective at reducing virus titer compared to the mock group. Western blot analysis suggested that si983 inhibited N gene expression and virus replication in a dose-dependent manner. These results indicate that N gene 983 sites are potential targets for antiviral therapy. Furthermore, RNAi targeting of the N gene may represent a viable method of treating against SCRV infection.
英文摘要: We evaluated the antiviral activity of several small interfering RNAs (siRNAs) corresponding to the Siniperca chuatsi rhabdovirus (SCRV) nucleoprotein (N) gene against the SCRV in a fish cell line, EPC. Among the siRNAs we examined, si983 was most effective at reducing virus titer compared to the mock group. Western blot analysis suggested that si983 inhibited N gene expression and virus replication in a dose-dependent manner. These results indicate that N gene 983 sites are potential targets for antiviral therapy. Furthermore, RNAi targeting of the N gene may represent a viable method of treating against SCRV infection.
关键词[WOS]: MANDARIN FISH ; SEQUENCE
语种: 英语
WOS记录号: WOS:000305037500007
ISSN号: 0388-788X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16927
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China

Recommended Citation:
Zhou, Guang-Zhou; Zhu, Rong; Gui, Jian-Fang; Zhang, Qi-Ya.Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line,FISH PATHOLOGY,2012,47(1):30-32
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文件名: Inhibition of Siniperca chuatsi.pdf
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