IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus
Yang, Yuhui1; Peng, Kou1; Liu, Xiaoyan1; Zhao, Dongmei1; Duan, Jundan1; Hu, Wei2; Zhao, Haobin1; Zhao, HB (reprint author), Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
2012-06-20
Source PublicationAQUACULTURE
ISSN0044-8486
Volume350Pages:1-7
AbstractMyostatin (MSTN), belonging to transforming growth factor (TGF) beta superfamily, is a negative regulator of muscular development in mammals and fish. The expression of MSTN can be regulated by hormone and environmental factors. Sex steroids, androgen and estrogen can affect sex differentiation and also can affect muscle development. However, few documents are concerned about the effects of sex steroids on MSTN expression in fish. Here, we report the cloning and expressional analyses of MSTN in rare minnow (Gobiocypris rarus), an emerging model fish in China. The MSTN cDNA of rare minnow that has an open reading frame of 1128 base pairs (bp) encodes a protein of 375 amino acids belonging to fish MSTN1 with typical structures like a prodomain, a mature MSTN domain, a conservative hydrolytic site (RIRR) and 9 conservative cysteine residues in mature MSTN. MSTN was detectable in all the examined tissues by RT-PCR that indicated the ubiquitous expression of MSTN in adult tissues. MSTN was detectable from zygote till hatching showed the maternal deposition of MSTN mRNA from oocytes and continuous expression during embryogenesis. Treatment of rare minnow with 1 mu g/L 17 alpha-methyl testosterone (MT) or 17 alpha-ethynyl estradiol (EE2) significantly impaired the body growth and increased the expression of MSTN mRNA detected by quantitative real time PCR. However, low dosage of MT (10 ng/L) had no significant effects on body growth and MSTN expression. The results suggest that the effects of exogenous sex steroids on growth can be mediated through MSTN. It is also suggested that low concentration of sex steroid (less than 10 ng/L) may have little effect on MSTN expression. (C) 2012 Elsevier B.V. All rights reserved.; Myostatin (MSTN), belonging to transforming growth factor (TGF) beta superfamily, is a negative regulator of muscular development in mammals and fish. The expression of MSTN can be regulated by hormone and environmental factors. Sex steroids, androgen and estrogen can affect sex differentiation and also can affect muscle development. However, few documents are concerned about the effects of sex steroids on MSTN expression in fish. Here, we report the cloning and expressional analyses of MSTN in rare minnow (Gobiocypris rarus), an emerging model fish in China. The MSTN cDNA of rare minnow that has an open reading frame of 1128 base pairs (bp) encodes a protein of 375 amino acids belonging to fish MSTN1 with typical structures like a prodomain, a mature MSTN domain, a conservative hydrolytic site (RIRR) and 9 conservative cysteine residues in mature MSTN. MSTN was detectable in all the examined tissues by RT-PCR that indicated the ubiquitous expression of MSTN in adult tissues. MSTN was detectable from zygote till hatching showed the maternal deposition of MSTN mRNA from oocytes and continuous expression during embryogenesis. Treatment of rare minnow with 1 mu g/L 17 alpha-methyl testosterone (MT) or 17 alpha-ethynyl estradiol (EE2) significantly impaired the body growth and increased the expression of MSTN mRNA detected by quantitative real time PCR. However, low dosage of MT (10 ng/L) had no significant effects on body growth and MSTN expression. The results suggest that the effects of exogenous sex steroids on growth can be mediated through MSTN. It is also suggested that low concentration of sex steroid (less than 10 ng/L) may have little effect on MSTN expression. (C) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordGobiocypris Rarus Myostatin Tempo-spatial Expression Real Time Pcr 17 Alpha-ethynyl Estradiol (Ee2) 17 Alpha-methyl Testosterone (Mt)
Department[Yang, Yuhui; Peng, Kou; Liu, Xiaoyan; Zhao, Dongmei; Duan, Jundan; Zhao, Haobin] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China; [Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430074, Peoples R China
DOI10.1016/j.aquaculture.2012.04.031
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationState Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2005FB18] ; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2005FB18] ; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2005FB18] ; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2005FB18]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000305068800001
WOS KeywordMESSENGER-RNA EXPRESSION ; ZEBRAFISH DANIO-RERIO ; RAINBOW-TROUT ; 17-ALPHA-ETHINYLESTRADIOL EE2 ; ONCORHYNCHUS-MYKISS ; BODY-COMPOSITION ; GENE-EXPRESSION ; SPARUS-AURATA ; MUSCLE MASS ; IGF-I
Funding OrganizationState Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2005FB18] ; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2005FB18] ; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2005FB18] ; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2005FB18]
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16925
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhao, HB (reprint author), Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
Affiliation1.Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430074, Peoples R China
Recommended Citation
GB/T 7714
Yang, Yuhui,Peng, Kou,Liu, Xiaoyan,et al. Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus[J]. AQUACULTURE,2012,350:1-7.
APA Yang, Yuhui.,Peng, Kou.,Liu, Xiaoyan.,Zhao, Dongmei.,Duan, Jundan.,...&Zhao, HB .(2012).Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus.AQUACULTURE,350,1-7.
MLA Yang, Yuhui,et al."Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus".AQUACULTURE 350(2012):1-7.
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