IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus
Cao, Mengxi1,2; Duan, Jundan2; Cheng, Nana2; Zhong, Xueping2; Wang, Zequn2; Hu, Wei1; Zhao, Haobin2; Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
2012-08-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
ISSN1095-6433
Volume162Issue:4Pages:303-309
AbstractFish have diverse sex determination and differentiation. DMRT1 and aromatase are conserved in the phyla and play pivotal roles in sex development. Gobiocypris rams is a small fish used as a model in aquatic toxicology in China and has been used to study the effects of environmental endocrine disruptors on gene expression, but its sexual development remains elusive. Here, we report the full-length cDNA of G. rams dmrt1 and its expression along with the expression of cyp19a1a and cyp19a1b, two genes encoding gonad and brain type aromatases, in adults and during ontogenesis. Both cyp19a1a and dmrt1 are expressed in the ovary and testis but show sexual dimorphism. Expression of cyp19a1a in the ovary is higher than in testes and dmrt1 follows the opposite pattern. Juvenile gonad histology changes at 15 days after hatching. The dimorphic expression of dmrt1 and cyp19a1a appears from 5 days after hatching, which is earlier than histological change. cyp19a1b is expressed coordinately with cyp19a1a until 15 days after hatching. These results show that dmrt1 and cyp19a1a play important roles in sex determination and sex differentiation in G. rams. (C) 2012 Elsevier Inc. All rights reserved.; Fish have diverse sex determination and differentiation. DMRT1 and aromatase are conserved in the phyla and play pivotal roles in sex development. Gobiocypris rams is a small fish used as a model in aquatic toxicology in China and has been used to study the effects of environmental endocrine disruptors on gene expression, but its sexual development remains elusive. Here, we report the full-length cDNA of G. rams dmrt1 and its expression along with the expression of cyp19a1a and cyp19a1b, two genes encoding gonad and brain type aromatases, in adults and during ontogenesis. Both cyp19a1a and dmrt1 are expressed in the ovary and testis but show sexual dimorphism. Expression of cyp19a1a in the ovary is higher than in testes and dmrt1 follows the opposite pattern. Juvenile gonad histology changes at 15 days after hatching. The dimorphic expression of dmrt1 and cyp19a1a appears from 5 days after hatching, which is earlier than histological change. cyp19a1b is expressed coordinately with cyp19a1a until 15 days after hatching. These results show that dmrt1 and cyp19a1a play important roles in sex determination and sex differentiation in G. rams. (C) 2012 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordGobiocypris Rarus Sex Determination And Sex Differentiation Dmrt1 Cyp19a1a Cyp19a1b Ontogenesis
Department[Cao, Mengxi; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; [Cao, Mengxi; Duan, Jundan; Cheng, Nana; Zhong, Xueping; Wang, Zequn; Zhao, Haobin] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China
DOI10.1016/j.cbpa.2012.03.021
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China[31140053]; Open Fund of the National Key Laboratory of Fresh Water Ecology and Biotechnology[2009FB18] ; National Natural Science Foundation of China[31140053]; Open Fund of the National Key Laboratory of Fresh Water Ecology and Biotechnology[2009FB18] ; National Natural Science Foundation of China[31140053]; Open Fund of the National Key Laboratory of Fresh Water Ecology and Biotechnology[2009FB18] ; National Natural Science Foundation of China[31140053]; Open Fund of the National Key Laboratory of Fresh Water Ecology and Biotechnology[2009FB18]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Physiology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Physiology ; Zoology
WOS IDWOS:000305726200003
WOS KeywordTILAPIA OREOCHROMIS-NILOTICUS ; GONADAL SEX-DIFFERENTIATION ; ORYZIAS-LATIPES ; CYTOCHROME-P450 AROMATASE ; ENVIRONMENTAL-INFLUENCES ; DETERMINING REGION ; TAKIFUGU-RUBRIPES ; DETERMINING GENE ; MESSENGER-RNA ; TELEOST FISH
Funding OrganizationNational Natural Science Foundation of China[31140053]; Open Fund of the National Key Laboratory of Fresh Water Ecology and Biotechnology[2009FB18] ; National Natural Science Foundation of China[31140053]; Open Fund of the National Key Laboratory of Fresh Water Ecology and Biotechnology[2009FB18] ; National Natural Science Foundation of China[31140053]; Open Fund of the National Key Laboratory of Fresh Water Ecology and Biotechnology[2009FB18] ; National Natural Science Foundation of China[31140053]; Open Fund of the National Key Laboratory of Fresh Water Ecology and Biotechnology[2009FB18]
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16919
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Cao, Mengxi,Duan, Jundan,Cheng, Nana,et al. Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2012,162(4):303-309.
APA Cao, Mengxi.,Duan, Jundan.,Cheng, Nana.,Zhong, Xueping.,Wang, Zequn.,...&Hu, W .(2012).Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,162(4),303-309.
MLA Cao, Mengxi,et al."Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 162.4(2012):303-309.
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