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题名: Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus
作者: Cao, Mengxi1, 2; Duan, Jundan2; Cheng, Nana2; Zhong, Xueping2; Wang, Zequn2; Hu, Wei1; Zhao, Haobin2
通讯作者: Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
关键词: Gobiocypris rarus ; Sex determination and sex differentiation ; dmrt1 ; cyp19a1a ; cyp19a1b ; Ontogenesis
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
发表日期: 2012-08-01
DOI: 10.1016/j.cbpa.2012.03.021
卷: 162, 期:4, 页:303-309
收录类别: SCI
文章类型: Article
部门归属: [Cao, Mengxi; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; [Cao, Mengxi; Duan, Jundan; Cheng, Nana; Zhong, Xueping; Wang, Zequn; Zhao, Haobin] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China[31140053]; Open Fund of the National Key Laboratory of Fresh Water Ecology and Biotechnology[2009FB18]
类目[WOS]: Biochemistry & Molecular Biology ; Physiology ; Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ; Physiology ; Zoology
摘要: Fish have diverse sex determination and differentiation. DMRT1 and aromatase are conserved in the phyla and play pivotal roles in sex development. Gobiocypris rams is a small fish used as a model in aquatic toxicology in China and has been used to study the effects of environmental endocrine disruptors on gene expression, but its sexual development remains elusive. Here, we report the full-length cDNA of G. rams dmrt1 and its expression along with the expression of cyp19a1a and cyp19a1b, two genes encoding gonad and brain type aromatases, in adults and during ontogenesis. Both cyp19a1a and dmrt1 are expressed in the ovary and testis but show sexual dimorphism. Expression of cyp19a1a in the ovary is higher than in testes and dmrt1 follows the opposite pattern. Juvenile gonad histology changes at 15 days after hatching. The dimorphic expression of dmrt1 and cyp19a1a appears from 5 days after hatching, which is earlier than histological change. cyp19a1b is expressed coordinately with cyp19a1a until 15 days after hatching. These results show that dmrt1 and cyp19a1a play important roles in sex determination and sex differentiation in G. rams. (C) 2012 Elsevier Inc. All rights reserved.
英文摘要: Fish have diverse sex determination and differentiation. DMRT1 and aromatase are conserved in the phyla and play pivotal roles in sex development. Gobiocypris rams is a small fish used as a model in aquatic toxicology in China and has been used to study the effects of environmental endocrine disruptors on gene expression, but its sexual development remains elusive. Here, we report the full-length cDNA of G. rams dmrt1 and its expression along with the expression of cyp19a1a and cyp19a1b, two genes encoding gonad and brain type aromatases, in adults and during ontogenesis. Both cyp19a1a and dmrt1 are expressed in the ovary and testis but show sexual dimorphism. Expression of cyp19a1a in the ovary is higher than in testes and dmrt1 follows the opposite pattern. Juvenile gonad histology changes at 15 days after hatching. The dimorphic expression of dmrt1 and cyp19a1a appears from 5 days after hatching, which is earlier than histological change. cyp19a1b is expressed coordinately with cyp19a1a until 15 days after hatching. These results show that dmrt1 and cyp19a1a play important roles in sex determination and sex differentiation in G. rams. (C) 2012 Elsevier Inc. All rights reserved.
关键词[WOS]: TILAPIA OREOCHROMIS-NILOTICUS ; GONADAL SEX-DIFFERENTIATION ; ORYZIAS-LATIPES ; CYTOCHROME-P450 AROMATASE ; ENVIRONMENTAL-INFLUENCES ; DETERMINING REGION ; TAKIFUGU-RUBRIPES ; DETERMINING GENE ; MESSENGER-RNA ; TELEOST FISH
语种: 英语
WOS记录号: WOS:000305726200003
ISSN号: 1095-6433
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16919
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China

Recommended Citation:
Cao, Mengxi; Duan, Jundan; Cheng, Nana; Zhong, Xueping; Wang, Zequn; Hu, Wei; Zhao, Haobin.Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2012,162(4):303-309
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