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Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara)
Lei, Xiao-Ying; Chen, Zhong-Yuan; He, Li-Bo; Pei, Chao; Yuan, Xiu-Ping; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2012-08-01
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN0920-1742
Volume38Issue:4Pages:1175-1182
AbstractA red-spotted grouper Epinephelus akaara skin (RGS) cell line was established and characterized. RGS cells had a normal diploid chromosome number of 2n = 48, the morphology of which was fibroblastic-like in 3 days and epithelial-like over 5 after 16 passages. The cells multiplied well in Dulbecco's modified Eagle's medium supplemented with 10% of fetal bovine serum at 25A degrees C. Susceptibilities of RGS and grass carp ovary (GCO) cells to two viruses were tested, and the results showed that the titer of an iridovirus Rana grylio virus (RGV) in RGS cells was 10(3.5) TCID50 ml(-1), which was much higher than a rhabdovirus spring viremia of carp virus (SVCV) in the cells (10(0.5) TCID50 ml(-1)). The titers of RGV and SVCV in GCO were 10(6.0) TCID50 ml(-1) and 10(8.0) TCID50 ml(-1), respectively, which were higher than those in RGS cells. The data may imply that RGS cells could be selectively resistible to some viruses during infection. RT-PCR analysis of RGV-infected RGS cells showed that RGV could replicate in RGS cells. Further study of virus replications in RGS cells was conducted by electron microscopy and immunofluorescence microscopy has shown that virus particles scattered in the cytoplasm and virus protein appeared in both the cytoplasm and nucleus. The results suggested that RGS cells could be used as a potential in vitro model to study the cutaneous barrier function against virus infection.; A red-spotted grouper Epinephelus akaara skin (RGS) cell line was established and characterized. RGS cells had a normal diploid chromosome number of 2n = 48, the morphology of which was fibroblastic-like in 3 days and epithelial-like over 5 after 16 passages. The cells multiplied well in Dulbecco's modified Eagle's medium supplemented with 10% of fetal bovine serum at 25A degrees C. Susceptibilities of RGS and grass carp ovary (GCO) cells to two viruses were tested, and the results showed that the titer of an iridovirus Rana grylio virus (RGV) in RGS cells was 10(3.5) TCID50 ml(-1), which was much higher than a rhabdovirus spring viremia of carp virus (SVCV) in the cells (10(0.5) TCID50 ml(-1)). The titers of RGV and SVCV in GCO were 10(6.0) TCID50 ml(-1) and 10(8.0) TCID50 ml(-1), respectively, which were higher than those in RGS cells. The data may imply that RGS cells could be selectively resistible to some viruses during infection. RT-PCR analysis of RGV-infected RGS cells showed that RGV could replicate in RGS cells. Further study of virus replications in RGS cells was conducted by electron microscopy and immunofluorescence microscopy has shown that virus particles scattered in the cytoplasm and virus protein appeared in both the cytoplasm and nucleus. The results suggested that RGS cells could be used as a potential in vitro model to study the cutaneous barrier function against virus infection.
SubtypeArticle
KeywordRed-spotted Grouper Epinephelus Akaara Skin Cell Line Chromosome Virus Susceptibility Virus Protein
Department[Lei, Xiao-Ying; Chen, Zhong-Yuan; He, Li-Bo; Pei, Chao; Yuan, Xiu-Ping; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1007/s10695-012-9603-7
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS SubjectBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS IDWOS:000305980200024
WOS KeywordRANA-GRYLIO VIRUS ; NF-KAPPA-B ; SPRING VIREMIA ; TELEOST FISH ; CARP VIRUS ; SEA BREAM ; ESTABLISHMENT ; COIOIDES ; IRIDOVIRUS ; ANTIBODIES
Funding OrganizationNational Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16]
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16913
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Lei, Xiao-Ying,Chen, Zhong-Yuan,He, Li-Bo,et al. Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2012,38(4):1175-1182.
APA Lei, Xiao-Ying.,Chen, Zhong-Yuan.,He, Li-Bo.,Pei, Chao.,Yuan, Xiu-Ping.,...&Zhang, QY .(2012).Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara).FISH PHYSIOLOGY AND BIOCHEMISTRY,38(4),1175-1182.
MLA Lei, Xiao-Ying,et al."Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara)".FISH PHYSIOLOGY AND BIOCHEMISTRY 38.4(2012):1175-1182.
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