IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.)
Zhong, Chengrong1,2; Song, Yanlong1,2; Wang, Yaping1; Li, Yongming1; Liao, Lanjie1; Xie, Shouqi1; Zhu, Zuoyan1; Hu, Wei1; Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2012-08-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume356Pages:404-411
AbstractHigher productivity of cultivated fish is required to sustain the supply of fisheries products in the light of increasing demand. We established different lines of homozygous growth hormone (GH) transgenic common carp and analyzed their biological characteristics. Comparing transgenic and control larvae derived from the same control fish mother, we found there was no significant difference in fertilization and hatch rates, but that hatch timing was advanced and average total length was significantly longer in transgenic carp. Early growth performance and other biological characteristics were compared among homozygous, hemizygous and control fish. Both the #TG2 and #TG3 lines exhibited greater average body weight, specific growth rate (SGR), feed conversion efficiency (FCE) and protein retention efficiency (PRE), as well as lower feeding rate (FR) and lipid and energy content than control fish. The energy retention efficiency (ERE) was significantly lower in the #TG3 line, while no difference was observed between the #TG2 line and control fish. These results indicate that the GH transgene initiated effects on growth enhancement in the early developmental stages and played an important role in improving protein synthesis and lipolysis. Conversely, homozygous transgenic fish No. 14960 grew faster than the control fish, but the culture traits of its hemizygous offspring (#TG1 line) were comparatively reduced, including lower SGR, FCE, PRE and ERE levels, and higher FR and lipid contents. Our results suggest that even if fast-growing GH-transgenic homozygous parental fish are generated, we still need to practice classical selective breeding in order to produce transgenic fish with improved traits that can meet aquaculture demand. (C) 2012 Elsevier B. V. All rights reserved.; Higher productivity of cultivated fish is required to sustain the supply of fisheries products in the light of increasing demand. We established different lines of homozygous growth hormone (GH) transgenic common carp and analyzed their biological characteristics. Comparing transgenic and control larvae derived from the same control fish mother, we found there was no significant difference in fertilization and hatch rates, but that hatch timing was advanced and average total length was significantly longer in transgenic carp. Early growth performance and other biological characteristics were compared among homozygous, hemizygous and control fish. Both the #TG2 and #TG3 lines exhibited greater average body weight, specific growth rate (SGR), feed conversion efficiency (FCE) and protein retention efficiency (PRE), as well as lower feeding rate (FR) and lipid and energy content than control fish. The energy retention efficiency (ERE) was significantly lower in the #TG3 line, while no difference was observed between the #TG2 line and control fish. These results indicate that the GH transgene initiated effects on growth enhancement in the early developmental stages and played an important role in improving protein synthesis and lipolysis. Conversely, homozygous transgenic fish No. 14960 grew faster than the control fish, but the culture traits of its hemizygous offspring (#TG1 line) were comparatively reduced, including lower SGR, FCE, PRE and ERE levels, and higher FR and lipid contents. Our results suggest that even if fast-growing GH-transgenic homozygous parental fish are generated, we still need to practice classical selective breeding in order to produce transgenic fish with improved traits that can meet aquaculture demand. (C) 2012 Elsevier B. V. All rights reserved.
SubtypeArticle
KeywordGrowth Hormone Transgenic Common Carp Homozygous Line Biological Characteristic Breed
Department[Zhong, Chengrong; Song, Yanlong; Wang, Yaping; Li, Yongming; Liao, Lanjie; Xie, Shouqi; Zhu, Zuoyan; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Zhong, Chengrong; Song, Yanlong] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
DOI10.1016/j.aquaculture.2012.04.019
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organization'863' High Technology Project[2011AA100404]; National Natural Science Foundation[30930069]; Chinese Academy of Sciences[KSCX2-EW-N-004] ; '863' High Technology Project[2011AA100404]; National Natural Science Foundation[30930069]; Chinese Academy of Sciences[KSCX2-EW-N-004] ; '863' High Technology Project[2011AA100404]; National Natural Science Foundation[30930069]; Chinese Academy of Sciences[KSCX2-EW-N-004] ; '863' High Technology Project[2011AA100404]; National Natural Science Foundation[30930069]; Chinese Academy of Sciences[KSCX2-EW-N-004]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000306171100051
WOS KeywordSALMON ONCORHYNCHUS-KISUTCH ; TILAPIA OREOCHROMIS-NILOTICUS ; ANTIFREEZE PROTEIN GENE ; TIME QUANTITATIVE PCR ; COHO SALMON ; ATLANTIC SALMON ; BODY-COMPOSITION ; CTENOPHARYNGODON-IDELLUS ; ZYGOSITY DETERMINATION ; MISGURNUS-MIZOLEPIS
Funding Organization'863' High Technology Project[2011AA100404]; National Natural Science Foundation[30930069]; Chinese Academy of Sciences[KSCX2-EW-N-004] ; '863' High Technology Project[2011AA100404]; National Natural Science Foundation[30930069]; Chinese Academy of Sciences[KSCX2-EW-N-004] ; '863' High Technology Project[2011AA100404]; National Natural Science Foundation[30930069]; Chinese Academy of Sciences[KSCX2-EW-N-004] ; '863' High Technology Project[2011AA100404]; National Natural Science Foundation[30930069]; Chinese Academy of Sciences[KSCX2-EW-N-004]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16911
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhong, Chengrong,Song, Yanlong,Wang, Yaping,et al. Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.)[J]. AQUACULTURE,2012,356:404-411.
APA Zhong, Chengrong.,Song, Yanlong.,Wang, Yaping.,Li, Yongming.,Liao, Lanjie.,...&Hu, W .(2012).Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.).AQUACULTURE,356,404-411.
MLA Zhong, Chengrong,et al."Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.)".AQUACULTURE 356(2012):404-411.
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