| Subcellular localization and functional characterization of a fish IRF9 from crucian carp Carassius auratus | |
| Shi, Jun; Zhang, Yi-Bing; Liu, Ting-Kai; Sun, Fan; Gui, Jian-Fang; Zhang, YB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Luojiashan St, Wuhan 430072, Peoples R China. | |
| 2012-08-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 33Issue:2Pages:258-266 |
| Abstract | Mammalian interferon (IFN) regulatory factor 9 (IRF-9) has long been recognized as the DNA sequence recognition subunit of IFN-stimulated gene factor 3 (ISGF3) complex, which is critical for type I IFN to induce the expression of IFN-stimulated genes (ISGs) against viral infection. Recent studies have shown that fish IFN exerts antiviral effects by induction of a number of ISGs and also of itself; however, little is known about the role of fish IRF9 in IFN signaling. Here we identify a fish IRF9 orthologue (CaIRF9) from IFN-producing cell line, crucian carp Carassius auratus blastulae embryonic (CAB) cells. Analysis of subcellular distribution of CaIRF9-green fluorescent protein indicates that CaIRF9 is constitutively present in the nucleus, which is driven by two nuclear localization signals (NLS), one locating within DNA-binding domain (DBD) of CaIRF9 and the other immediately behind DBD, although human IRF9 contains only one NLS analogous to the former of CaIRF9. Overexpression of CaIRF9 together with CaSTAT2 not only activates ISRE-containing promoter but also upregulates the expression of fish ISGs. Strikingly, CaIRF9 together with CaSTAT2 also exhibits an ability to activate crucian carp IFN promoter, and blockade of cellular CaIRF9 attenuates IFN itself-induced activation of crucian carp IFN promoter. Taken together, these data suggest that crucian carp IFN induces the expression of ISGs and also of itself possibly by the JAK-STAT signaling pathway that is conserved from fish to mammals. (C) 2012 Elsevier Ltd. All rights reserved.; Mammalian interferon (IFN) regulatory factor 9 (IRF-9) has long been recognized as the DNA sequence recognition subunit of IFN-stimulated gene factor 3 (ISGF3) complex, which is critical for type I IFN to induce the expression of IFN-stimulated genes (ISGs) against viral infection. Recent studies have shown that fish IFN exerts antiviral effects by induction of a number of ISGs and also of itself; however, little is known about the role of fish IRF9 in IFN signaling. Here we identify a fish IRF9 orthologue (CaIRF9) from IFN-producing cell line, crucian carp Carassius auratus blastulae embryonic (CAB) cells. Analysis of subcellular distribution of CaIRF9-green fluorescent protein indicates that CaIRF9 is constitutively present in the nucleus, which is driven by two nuclear localization signals (NLS), one locating within DNA-binding domain (DBD) of CaIRF9 and the other immediately behind DBD, although human IRF9 contains only one NLS analogous to the former of CaIRF9. Overexpression of CaIRF9 together with CaSTAT2 not only activates ISRE-containing promoter but also upregulates the expression of fish ISGs. Strikingly, CaIRF9 together with CaSTAT2 also exhibits an ability to activate crucian carp IFN promoter, and blockade of cellular CaIRF9 attenuates IFN itself-induced activation of crucian carp IFN promoter. Taken together, these data suggest that crucian carp IFN induces the expression of ISGs and also of itself possibly by the JAK-STAT signaling pathway that is conserved from fish to mammals. (C) 2012 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Interferon Regulator Factor 9 Nuclear Localization Signal Subcellular Localization Expression Regulation Jak-stat Signaling Pathway |
| Department | [Shi, Jun; Zhang, Yi-Bing; Liu, Ting-Kai; Sun, Fan; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China |
| DOI | 10.1016/j.fsi.2012.05.014 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China[31172435]; National Key Basic Research Program of China[2010CB126303]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2011FBZ17, 2011FBZ24] ; National Natural Science Foundation of China[31172435]; National Key Basic Research Program of China[2010CB126303]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2011FBZ17, 2011FBZ24] ; National Natural Science Foundation of China[31172435]; National Key Basic Research Program of China[2010CB126303]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2011FBZ17, 2011FBZ24] ; National Natural Science Foundation of China[31172435]; National Key Basic Research Program of China[2010CB126303]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2011FBZ17, 2011FBZ24] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000306632800012 |
| WOS Keyword | MULTIPLE REGULATORY DOMAINS ; DEPENDENT PROTEIN-KINASE ; PARALICHTHYS-OLIVACEUS ; CELLULAR-LOCALIZATION ; TRANSCRIPTION FACTORS ; ANTIVIRAL RESPONSE ; CELLS RESPOND ; IFN ; EXPRESSION ; VIRUS |
| Funding Organization | National Natural Science Foundation of China[31172435]; National Key Basic Research Program of China[2010CB126303]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2011FBZ17, 2011FBZ24] ; National Natural Science Foundation of China[31172435]; National Key Basic Research Program of China[2010CB126303]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2011FBZ17, 2011FBZ24] ; National Natural Science Foundation of China[31172435]; National Key Basic Research Program of China[2010CB126303]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2011FBZ17, 2011FBZ24] ; National Natural Science Foundation of China[31172435]; National Key Basic Research Program of China[2010CB126303]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2011FBZ17, 2011FBZ24] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/16907 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Zhang, YB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Luojiashan St, Wuhan 430072, Peoples R China. |
| Affiliation | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Shi, Jun,Zhang, Yi-Bing,Liu, Ting-Kai,et al. Subcellular localization and functional characterization of a fish IRF9 from crucian carp Carassius auratus[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(2):258-266. |
| APA | Shi, Jun,Zhang, Yi-Bing,Liu, Ting-Kai,Sun, Fan,Gui, Jian-Fang,&Zhang, YB .(2012).Subcellular localization and functional characterization of a fish IRF9 from crucian carp Carassius auratus.FISH & SHELLFISH IMMUNOLOGY,33(2),258-266. |
| MLA | Shi, Jun,et al."Subcellular localization and functional characterization of a fish IRF9 from crucian carp Carassius auratus".FISH & SHELLFISH IMMUNOLOGY 33.2(2012):258-266. |
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