IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome
Liu, Lusha1,2; Yu, Xiaomu1; Zhu, Chuankun1,2; Zeng, Fanzhen1,2; Tong, Jingou1; Tong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2012-09-01
Source PublicationCONSERVATION GENETICS RESOURCES
ISSN1877-7252
Volume4Issue:3Pages:699-701
AbstractIn this study, we constructed a genomic library to identify a set of new SNPs from the common carp (Cyprinus carpio L.) genome. Putative SNPs were validated by amplification of 41 fragments from the library using unrelated individuals. A total of 211 SNPs, 18 Indels and 6 microsatellites were identified from a total length of 18,244 bp genomic sequences. A/G was the most common substitution, and the ratio of transitions to transversions was 2.3:1. Ten validated SNPs were selected for genetic diversity analysis in three wild populations of common carp, and all loci were polymorphic, with the average observed (H (o)) and expected (H (e)) heterozygosities were 0.2154 and 0.3321, respectively. These new SNPs would be good resources for population genetics, parentage analysis and conservation genetics in common carp.; In this study, we constructed a genomic library to identify a set of new SNPs from the common carp (Cyprinus carpio L.) genome. Putative SNPs were validated by amplification of 41 fragments from the library using unrelated individuals. A total of 211 SNPs, 18 Indels and 6 microsatellites were identified from a total length of 18,244 bp genomic sequences. A/G was the most common substitution, and the ratio of transitions to transversions was 2.3:1. Ten validated SNPs were selected for genetic diversity analysis in three wild populations of common carp, and all loci were polymorphic, with the average observed (H (o)) and expected (H (e)) heterozygosities were 0.2154 and 0.3321, respectively. These new SNPs would be good resources for population genetics, parentage analysis and conservation genetics in common carp.
SubtypeArticle
KeywordCyprinus Carpio L. Single Nucleotide Polymorphisms (Snps) Validation Genetic Diversity
Department[Liu, Lusha; Yu, Xiaomu; Zhu, Chuankun; Zeng, Fanzhen; Tong, Jingou] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liu, Lusha; Zhu, Chuankun; Zeng, Fanzhen] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
DOI10.1007/s12686-012-9625-6
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMOA[200903045, 2011-G12]; MOST 973[2010CB126305]; FEBL of China[2011FBZ20] ; MOA[200903045, 2011-G12]; MOST 973[2010CB126305]; FEBL of China[2011FBZ20] ; MOA[200903045, 2011-G12]; MOST 973[2010CB126305]; FEBL of China[2011FBZ20] ; MOA[200903045, 2011-G12]; MOST 973[2010CB126305]; FEBL of China[2011FBZ20]
Indexed BySCI
Language英语
WOS Research AreaBiodiversity & Conservation ; Genetics & Heredity
WOS SubjectBiodiversity Conservation ; Genetics & Heredity
WOS IDWOS:000306586800037
Funding OrganizationMOA[200903045, 2011-G12]; MOST 973[2010CB126305]; FEBL of China[2011FBZ20] ; MOA[200903045, 2011-G12]; MOST 973[2010CB126305]; FEBL of China[2011FBZ20] ; MOA[200903045, 2011-G12]; MOST 973[2010CB126305]; FEBL of China[2011FBZ20] ; MOA[200903045, 2011-G12]; MOST 973[2010CB126305]; FEBL of China[2011FBZ20]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16905
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Liu, Lusha,Yu, Xiaomu,Zhu, Chuankun,et al. Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome[J]. CONSERVATION GENETICS RESOURCES,2012,4(3):699-701.
APA Liu, Lusha,Yu, Xiaomu,Zhu, Chuankun,Zeng, Fanzhen,Tong, Jingou,&Tong, JG .(2012).Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome.CONSERVATION GENETICS RESOURCES,4(3),699-701.
MLA Liu, Lusha,et al."Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome".CONSERVATION GENETICS RESOURCES 4.3(2012):699-701.
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