中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
题名: Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome
作者: Liu, Lusha1, 2; Yu, Xiaomu1; Zhu, Chuankun1, 2; Zeng, Fanzhen1, 2; Tong, Jingou1
通讯作者: Tong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Cyprinus carpio L. ; Single nucleotide polymorphisms (SNPs) ; Validation ; Genetic diversity
刊名: CONSERVATION GENETICS RESOURCES
发表日期: 2012-09-01
DOI: 10.1007/s12686-012-9625-6
卷: 4, 期:3, 页:699-701
收录类别: SCI
文章类型: Article
部门归属: [Liu, Lusha; Yu, Xiaomu; Zhu, Chuankun; Zeng, Fanzhen; Tong, Jingou] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liu, Lusha; Zhu, Chuankun; Zeng, Fanzhen] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: MOA[200903045, 2011-G12]; MOST 973[2010CB126305]; FEBL of China[2011FBZ20]
类目[WOS]: Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]: Biodiversity & Conservation ; Genetics & Heredity
摘要: In this study, we constructed a genomic library to identify a set of new SNPs from the common carp (Cyprinus carpio L.) genome. Putative SNPs were validated by amplification of 41 fragments from the library using unrelated individuals. A total of 211 SNPs, 18 Indels and 6 microsatellites were identified from a total length of 18,244 bp genomic sequences. A/G was the most common substitution, and the ratio of transitions to transversions was 2.3:1. Ten validated SNPs were selected for genetic diversity analysis in three wild populations of common carp, and all loci were polymorphic, with the average observed (H (o)) and expected (H (e)) heterozygosities were 0.2154 and 0.3321, respectively. These new SNPs would be good resources for population genetics, parentage analysis and conservation genetics in common carp.
英文摘要: In this study, we constructed a genomic library to identify a set of new SNPs from the common carp (Cyprinus carpio L.) genome. Putative SNPs were validated by amplification of 41 fragments from the library using unrelated individuals. A total of 211 SNPs, 18 Indels and 6 microsatellites were identified from a total length of 18,244 bp genomic sequences. A/G was the most common substitution, and the ratio of transitions to transversions was 2.3:1. Ten validated SNPs were selected for genetic diversity analysis in three wild populations of common carp, and all loci were polymorphic, with the average observed (H (o)) and expected (H (e)) heterozygosities were 0.2154 and 0.3321, respectively. These new SNPs would be good resources for population genetics, parentage analysis and conservation genetics in common carp.
语种: 英语
WOS记录号: WOS:000306586800037
ISSN号: 1877-7252
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16905
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome.pdf(168KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Liu, Lusha; Yu, Xiaomu; Zhu, Chuankun; Zeng, Fanzhen; Tong, Jingou.Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome,CONSERVATION GENETICS RESOURCES,2012,4(3):699-701
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Lusha]'s Articles
[Yu, Xiaomu]'s Articles
[Zhu, Chuankun]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Lusha]‘s Articles
[Yu, Xiaomu]‘s Articles
[Zhu, Chuankun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace