IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
Diversity of nifH gene amplified from plankton-community DNA in a shallow eutrophic lake (Lake Donghu, Wuhan, China)
Yan, Qingyun; Yu, Yuhe; Feng, Weisong; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, 7 Donghu S Rd, Wuhan 430072, Peoples R China.
2011
Source PublicationLIMNOLOGY
ISSN1439-8621
Volume12Issue:3Pages:245-251
AbstractBiological nitrogen fixation (BNF) is one of the major nitrogen inputs into the biosphere, and the nitrogenase iron protein (nifH) gene plays important roles in regulating the molecular nitrogen (N-2) fixation process. The nifH gene has also been extensively used to study the diversity and function of nitrogen-fixing microorganisms. In this study, we investigated the diversity of the nifH gene by culture-independent methods to analysis the planktonic nitrogen-fixing organisms in Lake Donghu, Wuhan, the largest urban lake in China. Results indicate that nifH gene sequences cloned from planktonic-community DNA showed high similarity to the uncultured cyanobacterial sequences deposited in the GenBank database. Phylogenetic analysis on the basis of the translated amino acid sequences further showed that most nifH clones were closely related to the reported cyanobacterial nifH gene sequences. Results also indicate that there are similar planktonic nitrogen-fixing organisms in the relatively independent areas of Lake Donghu, even though different regions showed a wide gradient in trophic status. These and other observations led us to believe that studies on nifH gene diversity and expression will increase our ability to understand the ecological function of target nitrogen-fixing groups in aquatic ecosystems.
KeywordNitrogenase Iron Protein (Nifh) Gene Biological Nitrogen Fixation (Bnf) Sequences Similarity Lake Donghu, Wuhan
Department[Yan, Qingyun; Yu, Yuhe; Feng, Weisong] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
Funding OrganizationNational Natural Science Foundation of China[30770298]; Key Laboratory of Mariculture of Ministry of Education, Ocean University of China[2009004] ; National Natural Science Foundation of China[30770298]; Key Laboratory of Mariculture of Ministry of Education, Ocean University of China[2009004] ; National Natural Science Foundation of China[30770298]; Key Laboratory of Mariculture of Ministry of Education, Ocean University of China[2009004] ; National Natural Science Foundation of China[30770298]; Key Laboratory of Mariculture of Ministry of Education, Ocean University of China[2009004]
Indexed BySCI
Language英语
WOS IDWOS:000300165100005
Funding OrganizationNational Natural Science Foundation of China[30770298]; Key Laboratory of Mariculture of Ministry of Education, Ocean University of China[2009004] ; National Natural Science Foundation of China[30770298]; Key Laboratory of Mariculture of Ministry of Education, Ocean University of China[2009004] ; National Natural Science Foundation of China[30770298]; Key Laboratory of Mariculture of Ministry of Education, Ocean University of China[2009004] ; National Natural Science Foundation of China[30770298]; Key Laboratory of Mariculture of Ministry of Education, Ocean University of China[2009004]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16891
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorYu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, 7 Donghu S Rd, Wuhan 430072, Peoples R China.
Recommended Citation
GB/T 7714
Yan, Qingyun,Yu, Yuhe,Feng, Weisong,et al. Diversity of nifH gene amplified from plankton-community DNA in a shallow eutrophic lake (Lake Donghu, Wuhan, China)[J]. LIMNOLOGY,2011,12(3):245-251.
APA Yan, Qingyun,Yu, Yuhe,Feng, Weisong,&Yu, YH .(2011).Diversity of nifH gene amplified from plankton-community DNA in a shallow eutrophic lake (Lake Donghu, Wuhan, China).LIMNOLOGY,12(3),245-251.
MLA Yan, Qingyun,et al."Diversity of nifH gene amplified from plankton-community DNA in a shallow eutrophic lake (Lake Donghu, Wuhan, China)".LIMNOLOGY 12.3(2011):245-251.
Files in This Item:
File Name/Size DocType Version Access License
Diversity of nifH ge(1763KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yan, Qingyun]'s Articles
[Yu, Yuhe]'s Articles
[Feng, Weisong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yan, Qingyun]'s Articles
[Yu, Yuhe]'s Articles
[Feng, Weisong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yan, Qingyun]'s Articles
[Yu, Yuhe]'s Articles
[Feng, Weisong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Diversity of nifH gene amplified from plankton-community DNA in a shallow eutrophic lake (Lake Donghu, Wuhan, China).pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.