中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
题名: Identification of cda gene in bighead carp and its expression in response to microcystin-LR
作者: Cai, Yan1; Li, Huiying1; Hao, Le1; Li, Guangyu1; Xie, Ping1; Chen, Jun1
通讯作者: Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
关键词: cda ; Gene identification ; mRNA expression ; Microcystin-LR ; Bighead carp
刊名: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
发表日期: 2012-05-01
DOI: 10.1016/j.ecoenv.2012.01.001
卷: 79, 期:-, 页:206-213
收录类别: SCI
文章类型: Article
部门归属: [Cai, Yan; Li, Huiying; Hao, Le; Li, Guangyu; Xie, Ping; Chen, Jun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
类目[WOS]: Environmental Sciences ; Toxicology
研究领域[WOS]: Environmental Sciences & Ecology ; Toxicology
摘要: Microcystin-LR (MCLR) is a widespread cyanotoxin, which can influence genes transcription and cause nucleic acid damage in different organisms. To identify MCLR induced transcriptionally changed hepatic genes in bighead carp by subtractive suppression hybridization, we obtained the cDNA fragment of cda. Then we cloned its full-length cDNA, which encodes a cytidine deaminase (CDA). 3D structure prediction showed that the 3D structure and amino acid residues related to function sites of bighead carp CDA were highly conserved. Bighead carp CDA shared high identities with other CDA sequences, and evolved closely to non-mammalian CDAs. Bighead carp expressed cda in all tested tissues under normal situation, and changed its expression profile in a time inversely dependent and dose dependent manner to MCLR, so as to protect itself from MCLR induced toxic damage. These indicated that cda might be involved in anti-MCLR response, especially in the regulation of cytidine and dexocytidine metabolism pathway. (C) 2012 Elsevier Inc. All rights reserved.
英文摘要: Microcystin-LR (MCLR) is a widespread cyanotoxin, which can influence genes transcription and cause nucleic acid damage in different organisms. To identify MCLR induced transcriptionally changed hepatic genes in bighead carp by subtractive suppression hybridization, we obtained the cDNA fragment of cda. Then we cloned its full-length cDNA, which encodes a cytidine deaminase (CDA). 3D structure prediction showed that the 3D structure and amino acid residues related to function sites of bighead carp CDA were highly conserved. Bighead carp CDA shared high identities with other CDA sequences, and evolved closely to non-mammalian CDAs. Bighead carp expressed cda in all tested tissues under normal situation, and changed its expression profile in a time inversely dependent and dose dependent manner to MCLR, so as to protect itself from MCLR induced toxic damage. These indicated that cda might be involved in anti-MCLR response, especially in the regulation of cytidine and dexocytidine metabolism pathway. (C) 2012 Elsevier Inc. All rights reserved.
关键词[WOS]: HUMAN CYTIDINE DEAMINASE ; CRYSTAL-STRUCTURE ; IN-VIVO ; ARABIDOPSIS-THALIANA ; CYTOSINE-ARABINOSIDE ; CDNA SEQUENCES ; DNA-DAMAGE ; PURIFICATION ; EVOLUTION ; ENZYME
语种: 英语
WOS记录号: WOS:000302107000029
ISSN号: 0147-6513
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16877
Appears in Collections:淡水生态学研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Identification of cda gene in bighead carp and its expression in response to microcystin-LR.pdf(1044KB)----开放获取--View 联系获取全文

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China

Recommended Citation:
Cai, Yan; Li, Huiying; Hao, Le; Li, Guangyu; Xie, Ping; Chen, Jun.Identification of cda gene in bighead carp and its expression in response to microcystin-LR,ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2012,79():206-213
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Cai, Yan]'s Articles
[Li, Huiying]'s Articles
[Hao, Le]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Cai, Yan]‘s Articles
[Li, Huiying]‘s Articles
[Hao, Le]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Identification of cda gene in bighead carp and its expression in response to microcystin-LR.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace