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Identification of cda gene in bighead carp and its expression in response to microcystin-LR
Cai, Yan; Li, Huiying; Hao, Le; Li, Guangyu; Xie, Ping; Chen, Jun; Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
2012-05-01
Source PublicationECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN0147-6513
Volume79Issue:-Pages:206-213
AbstractMicrocystin-LR (MCLR) is a widespread cyanotoxin, which can influence genes transcription and cause nucleic acid damage in different organisms. To identify MCLR induced transcriptionally changed hepatic genes in bighead carp by subtractive suppression hybridization, we obtained the cDNA fragment of cda. Then we cloned its full-length cDNA, which encodes a cytidine deaminase (CDA). 3D structure prediction showed that the 3D structure and amino acid residues related to function sites of bighead carp CDA were highly conserved. Bighead carp CDA shared high identities with other CDA sequences, and evolved closely to non-mammalian CDAs. Bighead carp expressed cda in all tested tissues under normal situation, and changed its expression profile in a time inversely dependent and dose dependent manner to MCLR, so as to protect itself from MCLR induced toxic damage. These indicated that cda might be involved in anti-MCLR response, especially in the regulation of cytidine and dexocytidine metabolism pathway. (C) 2012 Elsevier Inc. All rights reserved.; Microcystin-LR (MCLR) is a widespread cyanotoxin, which can influence genes transcription and cause nucleic acid damage in different organisms. To identify MCLR induced transcriptionally changed hepatic genes in bighead carp by subtractive suppression hybridization, we obtained the cDNA fragment of cda. Then we cloned its full-length cDNA, which encodes a cytidine deaminase (CDA). 3D structure prediction showed that the 3D structure and amino acid residues related to function sites of bighead carp CDA were highly conserved. Bighead carp CDA shared high identities with other CDA sequences, and evolved closely to non-mammalian CDAs. Bighead carp expressed cda in all tested tissues under normal situation, and changed its expression profile in a time inversely dependent and dose dependent manner to MCLR, so as to protect itself from MCLR induced toxic damage. These indicated that cda might be involved in anti-MCLR response, especially in the regulation of cytidine and dexocytidine metabolism pathway. (C) 2012 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordCda Gene Identification Mrna Expression Microcystin-lr Bighead Carp
Department[Cai, Yan; Li, Huiying; Hao, Le; Li, Guangyu; Xie, Ping; Chen, Jun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
DOI10.1016/j.ecoenv.2012.01.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000302107000029
WOS KeywordHUMAN CYTIDINE DEAMINASE ; CRYSTAL-STRUCTURE ; IN-VIVO ; ARABIDOPSIS-THALIANA ; CYTOSINE-ARABINOSIDE ; CDNA SEQUENCES ; DNA-DAMAGE ; PURIFICATION ; EVOLUTION ; ENZYME
Funding OrganizationNational Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16877
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorChen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Cai, Yan,Li, Huiying,Hao, Le,et al. Identification of cda gene in bighead carp and its expression in response to microcystin-LR[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2012,79(-):206-213.
APA Cai, Yan.,Li, Huiying.,Hao, Le.,Li, Guangyu.,Xie, Ping.,...&Chen, J .(2012).Identification of cda gene in bighead carp and its expression in response to microcystin-LR.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,79(-),206-213.
MLA Cai, Yan,et al."Identification of cda gene in bighead carp and its expression in response to microcystin-LR".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 79.-(2012):206-213.
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