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Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic-pituitary-thyroid axis in zebrafish larvae
Yan, Wei2; Zhou, Youxiang2; Yang, Jie2; Li, Shuqian2; Hu, Dingjin2; Wang, Jianghua3; Chen, Jun1; Li, Guangyu3; Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
2012-06-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume87Issue:11Pages:1301-1307
AbstractMicrocystin-leucine-arginine (MCLR) is the most toxic and the most commonly encountered variant of microcystins (MCs) in aquatic environment, and it has the potential for disrupting thyroid hormone homeostasis, but the molecular mechanisms underlying this process have not yet been clarified. In the present study, we observed body growth retardation associated with decreased levels of thyroid hormones (THs) in zebrafish larvae, highlighting the interferences of MCLR with the growth of fish larvae. To further our understanding of mechanisms of MCLR-induced endocrine toxicity, quantitative real-time PCR analysis was performed on hypothalamic-pituitary-thyroid (HPT) axis related genes of developing zebrafish embryos exposed to 100, 300 and 500 mu g L-1 MCLR until 96 h post-fertilization. The expression of several genes in the HPT system, i.e., corticotropin-releasing factor (CRF), thyroid-stimulating hormone (TSH), sodium/iodide symporter (NIS), thyroglobulin (TG), thyroid receptors (TR alpha and TR beta) and iodothyronine deiodinases (Dio1 and Dio2) was examined using quantitatively real-time PCR. The gene expression levels of CRF, TSH, NIS and TG were significantly induced after exposure to 500 mu g L-1 MCLR. The transcription of TRs gene was down-regulated in a concentration-dependent manner. Up-regulation and down-regulation of Deio1 and Deio2 gene expression, respectively, were observed upon exposure to MCLR. The above results indicated that MCLR could alter gene expression in the HPT axis which might subsequently contribute to MCLR-induced thyroid disruption. (C) 2012 Elsevier Ltd. All rights reserved.; Microcystin-leucine-arginine (MCLR) is the most toxic and the most commonly encountered variant of microcystins (MCs) in aquatic environment, and it has the potential for disrupting thyroid hormone homeostasis, but the molecular mechanisms underlying this process have not yet been clarified. In the present study, we observed body growth retardation associated with decreased levels of thyroid hormones (THs) in zebrafish larvae, highlighting the interferences of MCLR with the growth of fish larvae. To further our understanding of mechanisms of MCLR-induced endocrine toxicity, quantitative real-time PCR analysis was performed on hypothalamic-pituitary-thyroid (HPT) axis related genes of developing zebrafish embryos exposed to 100, 300 and 500 mu g L-1 MCLR until 96 h post-fertilization. The expression of several genes in the HPT system, i.e., corticotropin-releasing factor (CRF), thyroid-stimulating hormone (TSH), sodium/iodide symporter (NIS), thyroglobulin (TG), thyroid receptors (TR alpha and TR beta) and iodothyronine deiodinases (Dio1 and Dio2) was examined using quantitatively real-time PCR. The gene expression levels of CRF, TSH, NIS and TG were significantly induced after exposure to 500 mu g L-1 MCLR. The transcription of TRs gene was down-regulated in a concentration-dependent manner. Up-regulation and down-regulation of Deio1 and Deio2 gene expression, respectively, were observed upon exposure to MCLR. The above results indicated that MCLR could alter gene expression in the HPT axis which might subsequently contribute to MCLR-induced thyroid disruption. (C) 2012 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordMclr Zebrafish Gene Expression Hypothalamic-pituitary-thyroid Axis
Department[Chen, Jun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China; [Yan, Wei; Zhou, Youxiang; Yang, Jie; Li, Shuqian; Hu, Dingjin] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Wuhan 430064, Peoples R China; [Wang, Jianghua; Li, Guangyu] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
DOI10.1016/j.chemosphere.2012.01.041
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationFundamental Research Funds for the Central Universities[2011QC301]; State Key Laboratory of Freshwater Ecology and Biotechnology of China[2012FB09]; Natural Science Foundation of Hubei Province of China[2011CDB151]; National Nature Science Foundation of China (NSFC)[31000249]; Hubei Provincial Innovation Center for Agricultural Sciences and Technologies[2012-620-000-002] ; Fundamental Research Funds for the Central Universities[2011QC301]; State Key Laboratory of Freshwater Ecology and Biotechnology of China[2012FB09]; Natural Science Foundation of Hubei Province of China[2011CDB151]; National Nature Science Foundation of China (NSFC)[31000249]; Hubei Provincial Innovation Center for Agricultural Sciences and Technologies[2012-620-000-002] ; Fundamental Research Funds for the Central Universities[2011QC301]; State Key Laboratory of Freshwater Ecology and Biotechnology of China[2012FB09]; Natural Science Foundation of Hubei Province of China[2011CDB151]; National Nature Science Foundation of China (NSFC)[31000249]; Hubei Provincial Innovation Center for Agricultural Sciences and Technologies[2012-620-000-002] ; Fundamental Research Funds for the Central Universities[2011QC301]; State Key Laboratory of Freshwater Ecology and Biotechnology of China[2012FB09]; Natural Science Foundation of Hubei Province of China[2011CDB151]; National Nature Science Foundation of China (NSFC)[31000249]; Hubei Provincial Innovation Center for Agricultural Sciences and Technologies[2012-620-000-002]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000303640200016
WOS KeywordEMBRYONIC-DEVELOPMENT ; DANIO-RERIO ; DEIODINASE FAMILY ; RECEPTORS ; EXPRESSION ; CYANOBACTERIA ; HEPATOCYTES ; FISH ; DISRUPTION ; TOXINS
Funding OrganizationFundamental Research Funds for the Central Universities[2011QC301]; State Key Laboratory of Freshwater Ecology and Biotechnology of China[2012FB09]; Natural Science Foundation of Hubei Province of China[2011CDB151]; National Nature Science Foundation of China (NSFC)[31000249]; Hubei Provincial Innovation Center for Agricultural Sciences and Technologies[2012-620-000-002] ; Fundamental Research Funds for the Central Universities[2011QC301]; State Key Laboratory of Freshwater Ecology and Biotechnology of China[2012FB09]; Natural Science Foundation of Hubei Province of China[2011CDB151]; National Nature Science Foundation of China (NSFC)[31000249]; Hubei Provincial Innovation Center for Agricultural Sciences and Technologies[2012-620-000-002] ; Fundamental Research Funds for the Central Universities[2011QC301]; State Key Laboratory of Freshwater Ecology and Biotechnology of China[2012FB09]; Natural Science Foundation of Hubei Province of China[2011CDB151]; National Nature Science Foundation of China (NSFC)[31000249]; Hubei Provincial Innovation Center for Agricultural Sciences and Technologies[2012-620-000-002] ; Fundamental Research Funds for the Central Universities[2011QC301]; State Key Laboratory of Freshwater Ecology and Biotechnology of China[2012FB09]; Natural Science Foundation of Hubei Province of China[2011CDB151]; National Nature Science Foundation of China (NSFC)[31000249]; Hubei Provincial Innovation Center for Agricultural Sciences and Technologies[2012-620-000-002]
Citation statistics
Cited Times:57[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16861
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorChen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
2.Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Wuhan 430064, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Yan, Wei,Zhou, Youxiang,Yang, Jie,et al. Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic-pituitary-thyroid axis in zebrafish larvae[J]. CHEMOSPHERE,2012,87(11):1301-1307.
APA Yan, Wei.,Zhou, Youxiang.,Yang, Jie.,Li, Shuqian.,Hu, Dingjin.,...&Chen, J .(2012).Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic-pituitary-thyroid axis in zebrafish larvae.CHEMOSPHERE,87(11),1301-1307.
MLA Yan, Wei,et al."Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic-pituitary-thyroid axis in zebrafish larvae".CHEMOSPHERE 87.11(2012):1301-1307.
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