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Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp
He, Jun1; Chen, Jun1; Xie, Ping1; Zhang, Dawen1,3; Li, Guangyu1; Wu, Laiyan1; Zhang, Wei1; Guo, Xiaochun2; Li, Shangchun1; Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Donghu S Rd 7, Wuhan 430072, Peoples R China.
2012-07-15
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume116Issue:-Pages:61-68
AbstractGlutathione (GSH) and cysteine (Cys) conjugation have long been recognized to be important in the detoxification of microcystins (MCs) in animal organs, however, studies quantitatively estimating this process are rare, especially those simultaneously determining multiple toxins and their metabolites. This paper, for the first time, simultaneously quantified MC-LR (leucine arginine), MC-RR (arginine arginine), MCLR-GSH/Cys and MCRR-GSH/Cys in the liver, kidney, intestine and muscle of the cyanobacteria-eating bighead carp i.p. injected with two doses of MCs using liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). MCLR-Cys and MCRR-Cys content were much higher in kidney than in liver, intestine and muscle, suggesting the organotropism to kidney, while MCLR-GSH and MCRR-GSH were always below the detection limit. Bighead carp effectively metabolized MC-LR and MC-RR into the cysteine conjugates in kidney, as the ratios of MCLR-Cys to MC-LR and MCRR-Cys to MC-RR reached as high as 9.04 and 19.10, respectively. MC-LR and MC-RR were excreted mostly in the form of MCLR/RR-Cys rather than MCLR/RR-GSH, while MCs-GSH might act as mid-metabolites and changed to the more stable MCs-Cys rapidly. Cysteine conjugation of MCs appears to be an important biochemical mechanism for the cyanobacteria-eating fish to resist toxic cyanobacteria. A comparison of such detoxification mechanisms between fish and mammals would be interesting in the future studies. (C) 2012 Elsevier B.V. All rights reserved.; Glutathione (GSH) and cysteine (Cys) conjugation have long been recognized to be important in the detoxification of microcystins (MCs) in animal organs, however, studies quantitatively estimating this process are rare, especially those simultaneously determining multiple toxins and their metabolites. This paper, for the first time, simultaneously quantified MC-LR (leucine arginine), MC-RR (arginine arginine), MCLR-GSH/Cys and MCRR-GSH/Cys in the liver, kidney, intestine and muscle of the cyanobacteria-eating bighead carp i.p. injected with two doses of MCs using liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). MCLR-Cys and MCRR-Cys content were much higher in kidney than in liver, intestine and muscle, suggesting the organotropism to kidney, while MCLR-GSH and MCRR-GSH were always below the detection limit. Bighead carp effectively metabolized MC-LR and MC-RR into the cysteine conjugates in kidney, as the ratios of MCLR-Cys to MC-LR and MCRR-Cys to MC-RR reached as high as 9.04 and 19.10, respectively. MC-LR and MC-RR were excreted mostly in the form of MCLR/RR-Cys rather than MCLR/RR-GSH, while MCs-GSH might act as mid-metabolites and changed to the more stable MCs-Cys rapidly. Cysteine conjugation of MCs appears to be an important biochemical mechanism for the cyanobacteria-eating fish to resist toxic cyanobacteria. A comparison of such detoxification mechanisms between fish and mammals would be interesting in the future studies. (C) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordQuantification Mclr-gsh/cys Mcrr-gsh/cys Bighead Carp
Department[He, Jun; Chen, Jun; Xie, Ping; Zhang, Dawen; Li, Guangyu; Wu, Laiyan; Zhang, Wei; Li, Shangchun] Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China; [Guo, Xiaochun] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China; [Zhang, Dawen] Jiang Xi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Peoples R China
DOI10.1016/j.aquatox.2012.03.004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000304290900009
WOS KeywordTANDEM MASS-SPECTROMETRY ; PROTEIN PHOSPHATASE-1 ; INHIBITORY-ACTIVITY ; LR ; TOXICITY ; LIVER ; FISH ; RR ; IDENTIFICATION ; METABOLITES
Funding OrganizationNational Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077]
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16859
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorChen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Donghu S Rd 7, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
3.Jiang Xi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Peoples R China
Recommended Citation
GB/T 7714
He, Jun,Chen, Jun,Xie, Ping,et al. Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp[J]. AQUATIC TOXICOLOGY,2012,116(-):61-68.
APA He, Jun.,Chen, Jun.,Xie, Ping.,Zhang, Dawen.,Li, Guangyu.,...&Chen, J .(2012).Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp.AQUATIC TOXICOLOGY,116(-),61-68.
MLA He, Jun,et al."Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp".AQUATIC TOXICOLOGY 116.-(2012):61-68.
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