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题名: Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp
作者: He, Jun1; Chen, Jun1; Xie, Ping1; Zhang, Dawen1, 3; Li, Guangyu1; Wu, Laiyan1; Zhang, Wei1; Guo, Xiaochun2; Li, Shangchun1
通讯作者: Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Donghu S Rd 7, Wuhan 430072, Peoples R China.
关键词: Quantification ; MCLR-GSH/Cys ; MCRR-GSH/Cys ; Bighead carp
刊名: AQUATIC TOXICOLOGY
发表日期: 2012-07-15
DOI: 10.1016/j.aquatox.2012.03.004
卷: 116, 期:-, 页:61-68
收录类别: SCI
文章类型: Article
部门归属: [He, Jun; Chen, Jun; Xie, Ping; Zhang, Dawen; Li, Guangyu; Wu, Laiyan; Zhang, Wei; Li, Shangchun] Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China; [Guo, Xiaochun] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China; [Zhang, Dawen] Jiang Xi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundations of China[31070457, 30700077]
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: Glutathione (GSH) and cysteine (Cys) conjugation have long been recognized to be important in the detoxification of microcystins (MCs) in animal organs, however, studies quantitatively estimating this process are rare, especially those simultaneously determining multiple toxins and their metabolites. This paper, for the first time, simultaneously quantified MC-LR (leucine arginine), MC-RR (arginine arginine), MCLR-GSH/Cys and MCRR-GSH/Cys in the liver, kidney, intestine and muscle of the cyanobacteria-eating bighead carp i.p. injected with two doses of MCs using liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). MCLR-Cys and MCRR-Cys content were much higher in kidney than in liver, intestine and muscle, suggesting the organotropism to kidney, while MCLR-GSH and MCRR-GSH were always below the detection limit. Bighead carp effectively metabolized MC-LR and MC-RR into the cysteine conjugates in kidney, as the ratios of MCLR-Cys to MC-LR and MCRR-Cys to MC-RR reached as high as 9.04 and 19.10, respectively. MC-LR and MC-RR were excreted mostly in the form of MCLR/RR-Cys rather than MCLR/RR-GSH, while MCs-GSH might act as mid-metabolites and changed to the more stable MCs-Cys rapidly. Cysteine conjugation of MCs appears to be an important biochemical mechanism for the cyanobacteria-eating fish to resist toxic cyanobacteria. A comparison of such detoxification mechanisms between fish and mammals would be interesting in the future studies. (C) 2012 Elsevier B.V. All rights reserved.
英文摘要: Glutathione (GSH) and cysteine (Cys) conjugation have long been recognized to be important in the detoxification of microcystins (MCs) in animal organs, however, studies quantitatively estimating this process are rare, especially those simultaneously determining multiple toxins and their metabolites. This paper, for the first time, simultaneously quantified MC-LR (leucine arginine), MC-RR (arginine arginine), MCLR-GSH/Cys and MCRR-GSH/Cys in the liver, kidney, intestine and muscle of the cyanobacteria-eating bighead carp i.p. injected with two doses of MCs using liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). MCLR-Cys and MCRR-Cys content were much higher in kidney than in liver, intestine and muscle, suggesting the organotropism to kidney, while MCLR-GSH and MCRR-GSH were always below the detection limit. Bighead carp effectively metabolized MC-LR and MC-RR into the cysteine conjugates in kidney, as the ratios of MCLR-Cys to MC-LR and MCRR-Cys to MC-RR reached as high as 9.04 and 19.10, respectively. MC-LR and MC-RR were excreted mostly in the form of MCLR/RR-Cys rather than MCLR/RR-GSH, while MCs-GSH might act as mid-metabolites and changed to the more stable MCs-Cys rapidly. Cysteine conjugation of MCs appears to be an important biochemical mechanism for the cyanobacteria-eating fish to resist toxic cyanobacteria. A comparison of such detoxification mechanisms between fish and mammals would be interesting in the future studies. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ; PROTEIN PHOSPHATASE-1 ; INHIBITORY-ACTIVITY ; LR ; TOXICITY ; LIVER ; FISH ; RR ; IDENTIFICATION ; METABOLITES
语种: 英语
WOS记录号: WOS:000304290900009
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16859
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
3.Jiang Xi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Peoples R China

Recommended Citation:
He, Jun; Chen, Jun; Xie, Ping; Zhang, Dawen; Li, Guangyu; Wu, Laiyan; Zhang, Wei; Guo, Xiaochun; Li, Shangchun.Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp,AQUATIC TOXICOLOGY,2012,116():61-68
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