IHB OpenIR  > 水生生物多样性与资源保护研究中心  > 期刊论文
Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise
Wang, Jingzhen; Su, Weiting; Nie, Wenhui; Wang, Jinhuan; Xiao, Wuhan; Wang, Ding; Xiao, WH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China.
2011
Source PublicationIN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
ISSN1071-2690
Volume47Issue:9Pages:618-630
AbstractThe Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis), as the sole freshwater subspecies of N. phocaenoides, is endemic to the Yangtze River and its adjacent lakes. Its population has declined significantly over recent decades. In this study, we established a skin-derived finite fibroblast cell line of the Yangtze finless porpoise, named YFP-SF1, using primary cell culture methods, and an immortalized cell line, T-YFP-SF1, through co-transfection (GFP and SV40 T antigens) techniques. YFP-SF1 proliferated continuously with a minimum population doubling time of 31 h and exhibited age-dependent changes in growth rate. T-YFP-SF1 cells exhibited fibroblast morphology and were characterized by a shorter doubling time, higher attachment efficiencies, colony formation at a low seeding density, and growth in low serum concentrations. Anchorage independence and foci formation in the cell monolayer were observed from passage 36. The chromosome number of YFP-SF1 and T-YFP-SF1 remained stable at 2n=44 in the early passages, and the viability of thawed cells remained above 90% after cryopreservation in liquid nitrogen. Taken together, we have established fibroblast cell lines of Yangtze finless porpoise for the first time, which might assist as an in vitro model for this endangered mammal.
KeywordFinless Porpoise Fibroblast Cell Line Co-transfection In Vitro
Department[Wang, Jingzhen; Xiao, Wuhan; Wang, Ding] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China; [Su, Weiting; Nie, Wenhui; Wang, Jinhuan] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China
Funding OrganizationNational Key Basic Research Foundation[2007CB411600]; National Natural Science Foundation of China[30730018]; State Key Laboratory of Genetic Resources and Evolution[GREKF08-06] ; National Key Basic Research Foundation[2007CB411600]; National Natural Science Foundation of China[30730018]; State Key Laboratory of Genetic Resources and Evolution[GREKF08-06] ; National Key Basic Research Foundation[2007CB411600]; National Natural Science Foundation of China[30730018]; State Key Laboratory of Genetic Resources and Evolution[GREKF08-06] ; National Key Basic Research Foundation[2007CB411600]; National Natural Science Foundation of China[30730018]; State Key Laboratory of Genetic Resources and Evolution[GREKF08-06]
Indexed BySCI
Language英语
WOS IDWOS:000298230100004
Funding OrganizationNational Key Basic Research Foundation[2007CB411600]; National Natural Science Foundation of China[30730018]; State Key Laboratory of Genetic Resources and Evolution[GREKF08-06] ; National Key Basic Research Foundation[2007CB411600]; National Natural Science Foundation of China[30730018]; State Key Laboratory of Genetic Resources and Evolution[GREKF08-06] ; National Key Basic Research Foundation[2007CB411600]; National Natural Science Foundation of China[30730018]; State Key Laboratory of Genetic Resources and Evolution[GREKF08-06] ; National Key Basic Research Foundation[2007CB411600]; National Natural Science Foundation of China[30730018]; State Key Laboratory of Genetic Resources and Evolution[GREKF08-06]
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16823
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorXiao, WH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China.
Recommended Citation
GB/T 7714
Wang, Jingzhen,Su, Weiting,Nie, Wenhui,et al. Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2011,47(9):618-630.
APA Wang, Jingzhen.,Su, Weiting.,Nie, Wenhui.,Wang, Jinhuan.,Xiao, Wuhan.,...&Xiao, WH .(2011).Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,47(9),618-630.
MLA Wang, Jingzhen,et al."Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47.9(2011):618-630.
Files in This Item:
File Name/Size DocType Version Access License
Establishment and ch(966KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Jingzhen]'s Articles
[Su, Weiting]'s Articles
[Nie, Wenhui]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Jingzhen]'s Articles
[Su, Weiting]'s Articles
[Nie, Wenhui]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Jingzhen]'s Articles
[Su, Weiting]'s Articles
[Nie, Wenhui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.