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Analysis of the bacterial diversity in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis
McLaughlin, Richard William1,2; Chen, MinMin1,3; Zheng, JinSong1,4; Zhao, QingZhong1,4; Wang, Ding1,4; Zheng, JS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2012-05-01
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume39Issue:5Pages:5669-5676
AbstractThe aim of this study was to determine the bacteria present in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis. Fecal samples were collected from 12 Yangtze finless porpoises living in the wild at Poyang Lake, located in Jiangxi Province, China. To determine the bacterial diversity, a 16S rRNA gene clone library using the bacterial PCR primers fD1 and rP2, was prepared. A total of 138 near-full-length sequences were analyzed and 39 operational taxonomic units (OTUs) were identified. Sequences showing a parts per thousand yen97% similarity were grouped together as an OTU. Six different phyla were identified in which 38 OTUs were classified. Most of the OTUs contained sequences belonged to the phylum Firmicutes (51.3%), followed by Tenericutes (17.9%), Proteobacteria (15.4%), Actinobacteria (7.7%), Deinococcus-Thermus (2.6%) and Cyanobacteria (2.6%). A phylum could not be assigned for one clone within one OTU (2.6%). It appears that the Yangtze finless porpoise has a more diverse range of bacteria compared to other aquatic mammals, such as seals.; The aim of this study was to determine the bacteria present in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis. Fecal samples were collected from 12 Yangtze finless porpoises living in the wild at Poyang Lake, located in Jiangxi Province, China. To determine the bacterial diversity, a 16S rRNA gene clone library using the bacterial PCR primers fD1 and rP2, was prepared. A total of 138 near-full-length sequences were analyzed and 39 operational taxonomic units (OTUs) were identified. Sequences showing a parts per thousand yen97% similarity were grouped together as an OTU. Six different phyla were identified in which 38 OTUs were classified. Most of the OTUs contained sequences belonged to the phylum Firmicutes (51.3%), followed by Tenericutes (17.9%), Proteobacteria (15.4%), Actinobacteria (7.7%), Deinococcus-Thermus (2.6%) and Cyanobacteria (2.6%). A phylum could not be assigned for one clone within one OTU (2.6%). It appears that the Yangtze finless porpoise has a more diverse range of bacteria compared to other aquatic mammals, such as seals.
SubtypeArticle
KeywordYangtze Finless Porpoise (Neophocaena Phocaenoides Asiaeorientalis) 16s Rrna Gene Cetacean Bacteria Feces
Department[McLaughlin, Richard William; Chen, MinMin; Zheng, JinSong; Zhao, QingZhong; Wang, Ding] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [McLaughlin, Richard William] St Marys Univ Minnesota, Dept Biol, Winona, MN 55987 USA; [Chen, MinMin] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Zheng, JinSong; Zhao, QingZhong; Wang, Ding] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
DOI10.1007/s11033-011-1375-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China[30730018, 31000168]; National Basic Research Program of China[2007CB411600] ; National Natural Science Foundation of China[30730018, 31000168]; National Basic Research Program of China[2007CB411600] ; National Natural Science Foundation of China[30730018, 31000168]; National Basic Research Program of China[2007CB411600] ; National Natural Science Foundation of China[30730018, 31000168]; National Basic Research Program of China[2007CB411600]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000302147800071
WOS KeywordCULTURE-INDEPENDENT ANALYSIS ; GAYALS BOS-FRONTALIS ; GASTROINTESTINAL-TRACT ; PHYLOGENETIC ANALYSIS ; MICROBIAL DIVERSITY ; SEQUENCE-ANALYSIS ; INTESTINAL-TRACT ; RUMEN ; CATTLE ; DNA
Funding OrganizationNational Natural Science Foundation of China[30730018, 31000168]; National Basic Research Program of China[2007CB411600] ; National Natural Science Foundation of China[30730018, 31000168]; National Basic Research Program of China[2007CB411600] ; National Natural Science Foundation of China[30730018, 31000168]; National Basic Research Program of China[2007CB411600] ; National Natural Science Foundation of China[30730018, 31000168]; National Basic Research Program of China[2007CB411600]
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Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16817
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorZheng, JS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.St Marys Univ Minnesota, Dept Biol, Winona, MN 55987 USA
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
McLaughlin, Richard William,Chen, MinMin,Zheng, JinSong,et al. Analysis of the bacterial diversity in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis[J]. MOLECULAR BIOLOGY REPORTS,2012,39(5):5669-5676.
APA McLaughlin, Richard William,Chen, MinMin,Zheng, JinSong,Zhao, QingZhong,Wang, Ding,&Zheng, JS .(2012).Analysis of the bacterial diversity in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis.MOLECULAR BIOLOGY REPORTS,39(5),5669-5676.
MLA McLaughlin, Richard William,et al."Analysis of the bacterial diversity in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis".MOLECULAR BIOLOGY REPORTS 39.5(2012):5669-5676.
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