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The Cyanobacterial NAD Kinase Gene sll1415 Is Required for Photoheterotrophic Growth and Cellular Redox Homeostasis in Synechocystis sp Strain PCC 6803
Gao, Hong; Xu, Xudong; Xu, XD (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2012
Source PublicationJOURNAL OF BACTERIOLOGY
ISSN0021-9193
Volume194Issue:2Pages:218-224
AbstractNAD kinase (NADK), which phosphorylates NAD to NADP, is one of the key enzymes regulating the cellular NADP( H) level. In Synechocystis sp. strain PCC 6803, slr0400 and sll1415 were shown to encode NAD kinases. The NADP( H) pool in the cyanobacterium was remarkably reduced by an sll1415-null mutation but slightly reduced by an slr0400-null mutation. The reduction of the NADP( H) level in the sll1415 mutant led to a significant accumulation of glucose-6-phosphate and a loss of photoheterotrophic growth. As the primary NADK gene, sll1415 was found to inhibit the transcription of genes involved in redox homeostasis and to exert stronger effects on methyl viologen tolerance than slr0040.; NAD kinase (NADK), which phosphorylates NAD to NADP, is one of the key enzymes regulating the cellular NADP( H) level. In Synechocystis sp. strain PCC 6803, slr0400 and sll1415 were shown to encode NAD kinases. The NADP( H) pool in the cyanobacterium was remarkably reduced by an sll1415-null mutation but slightly reduced by an slr0400-null mutation. The reduction of the NADP( H) level in the sll1415 mutant led to a significant accumulation of glucose-6-phosphate and a loss of photoheterotrophic growth. As the primary NADK gene, sll1415 was found to inhibit the transcription of genes involved in redox homeostasis and to exert stronger effects on methyl viologen tolerance than slr0040.
SubtypeArticle
Department[Gao, Hong; Xu, Xudong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
DOI10.1128/JB.05873-11
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China[30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30825003]; State Key Basic Research Development Program of China[2008CB418001]
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000298677400003
WOS KeywordSITE-DIRECTED MUTAGENESIS ; SACCHAROMYCES-CEREVISIAE ; SP PCC-6803 ; OXIDATIVE STRESS ; COMPLEX ; BINDING ; NADP(H) ; ENZYME ; ATP
Funding OrganizationNational Natural Science Foundation of China[30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30825003]; State Key Basic Research Development Program of China[2008CB418001]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16773
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, XD (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
AffiliationChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Gao, Hong,Xu, Xudong,Xu, XD . The Cyanobacterial NAD Kinase Gene sll1415 Is Required for Photoheterotrophic Growth and Cellular Redox Homeostasis in Synechocystis sp Strain PCC 6803[J]. JOURNAL OF BACTERIOLOGY,2012,194(2):218-224.
APA Gao, Hong,Xu, Xudong,&Xu, XD .(2012).The Cyanobacterial NAD Kinase Gene sll1415 Is Required for Photoheterotrophic Growth and Cellular Redox Homeostasis in Synechocystis sp Strain PCC 6803.JOURNAL OF BACTERIOLOGY,194(2),218-224.
MLA Gao, Hong,et al."The Cyanobacterial NAD Kinase Gene sll1415 Is Required for Photoheterotrophic Growth and Cellular Redox Homeostasis in Synechocystis sp Strain PCC 6803".JOURNAL OF BACTERIOLOGY 194.2(2012):218-224.
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