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题名: 14-3-3 zeta Interacts with Stat3 and Regulates Its Constitutive Activation in Multiple Myeloma Cells
作者: Zhang, Jia1; Chen, Fangjin2; Li, Wenliang3; Xiong, Qian1; Yang, Mingkun1; Zheng, Peng1; Li, Chongyang1; Pei, Jianfeng2; Ge, Feng1
通讯作者: Zhang, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
刊名: PLOS ONE
发表日期: 2012-01-18
DOI: 10.1371/journal.pone.0029554
卷: 7, 期:1, 页:e29554
收录类别: SCI
文章类型: Article
部门归属: [Zhang, Jia; Xiong, Qian; Yang, Mingkun; Zheng, Peng; Li, Chongyang; Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; [Chen, Fangjin; Pei, Jianfeng] Peking Univ, Ctr Theoret Biol, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China; [Li, Wenliang] Tokai Univ, Sch Sci & Technol, Tokyo 151, Japan
WOS标题词: Science & Technology
资助者: Chinese Academy of Sciences; Japan Society for the Promotion of Science
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: The 14-3-3 proteins are a family of regulatory signaling molecules that interact with other proteins in a phosphorylation-dependent manner and function as adapter or scaffold proteins in signal transduction pathways. One family member, 14-3-3 zeta, is believed to function in cell signaling, cycle control, and apoptotic death. A systematic proteomic analysis done in our laboratory has identified signal transducers and activators of transcription 3 (Stat3) as a novel 14-3-3 zeta interacting protein. Following our initial finding, in this study, we provide evidence that 14-3-3 zeta interacts physically with Stat3. We further demonstrate that phosphorylation of Stat3 at Ser727 is vital for 14-3-3 zeta interaction and mutation of Ser727 to Alanine abolished 14-3-3 zeta/Stat3 association. Inhibition of 14-3-3 zeta protein expression in U266 cells inhibited Stat3 Ser727 phosphorylation and nuclear translocation, and decreased both Stat3 DNA binding and transcriptional activity. Moreover, 14-3-3 zeta is involved in the regulation of protein kinase C (PKC) activity and 14-3-3 zeta binding to Stat3 protects Ser727 dephosphorylation from protein phosphatase 2A (PP2A). Taken together, our findings support the model that multiple signaling events impinge on Stat3 and that 14-3-3 zeta serves as an essential coordinator for different pathways to regulate Stat3 activation and function in MM cells.
英文摘要: The 14-3-3 proteins are a family of regulatory signaling molecules that interact with other proteins in a phosphorylation-dependent manner and function as adapter or scaffold proteins in signal transduction pathways. One family member, 14-3-3 zeta, is believed to function in cell signaling, cycle control, and apoptotic death. A systematic proteomic analysis done in our laboratory has identified signal transducers and activators of transcription 3 (Stat3) as a novel 14-3-3 zeta interacting protein. Following our initial finding, in this study, we provide evidence that 14-3-3 zeta interacts physically with Stat3. We further demonstrate that phosphorylation of Stat3 at Ser727 is vital for 14-3-3 zeta interaction and mutation of Ser727 to Alanine abolished 14-3-3 zeta/Stat3 association. Inhibition of 14-3-3 zeta protein expression in U266 cells inhibited Stat3 Ser727 phosphorylation and nuclear translocation, and decreased both Stat3 DNA binding and transcriptional activity. Moreover, 14-3-3 zeta is involved in the regulation of protein kinase C (PKC) activity and 14-3-3 zeta binding to Stat3 protects Ser727 dephosphorylation from protein phosphatase 2A (PP2A). Taken together, our findings support the model that multiple signaling events impinge on Stat3 and that 14-3-3 zeta serves as an essential coordinator for different pathways to regulate Stat3 activation and function in MM cells.
关键词[WOS]: PROTEIN-KINASE-C ; PHOSPHORYLATION-DEPENDENT BINDING ; MM-PBSA METHOD ; IN-VIVO ; PROSTATE-CANCER ; PHOSPHATASE 2A ; APOPTOSIS ; RAF-1 ; 14-3-3-PROTEINS ; IDENTIFICATION
语种: 英语
WOS记录号: WOS:000299771900018
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16765
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
2.Peking Univ, Ctr Theoret Biol, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
3.Tokai Univ, Sch Sci & Technol, Tokyo 151, Japan

Recommended Citation:
Zhang, Jia; Chen, Fangjin; Li, Wenliang; Xiong, Qian; Yang, Mingkun; Zheng, Peng; Li, Chongyang; Pei, Jianfeng; Ge, Feng.14-3-3 zeta Interacts with Stat3 and Regulates Its Constitutive Activation in Multiple Myeloma Cells,PLOS ONE,2012,7(1):e29554
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