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Evaluation of the Role of Functional Constraints on the Integrity of an Ultraconserved Region in the Genus Drosophila
Diaz-Castillo, Carlos1; Xia, Xiao-Qin2; Ranz, Jose M.1; Diaz-Castillo, C (reprint author), Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA
2012-02-01
Source PublicationPLOS GENETICS
ISSN1553-7404
Volume8Issue:2Pages:e1002475
AbstractWhy gene order is conserved over long evolutionary timespans remains elusive. A common interpretation is that gene order conservation might reflect the existence of functional constraints that are important for organismal performance. Alteration of the integrity of genomic regions, and therefore of those constraints, would result in detrimental effects. This notion seems especially plausible in those genomes that can easily accommodate gene reshuffling via chromosomal inversions since genomic regions free of constraints are likely to have been disrupted in one or more lineages. Nevertheless, no empirical test has been performed to this notion. Here, we disrupt one of the largest conserved genomic regions of the Drosophila genome by chromosome engineering and examine the phenotypic consequences derived from such disruption. The targeted region exhibits multiple patterns of functional enrichment suggestive of the presence of constraints. The carriers of the disrupted collinear block show no defects in their viability, fertility, and parameters of general homeostasis, although their odorant perception is altered. This change in odorant perception does not correlate with modifications of the level of expression and sex bias of the genes within the genomic region disrupted. Our results indicate that even in highly rearranged genomes, like those of Diptera, unusually high levels of gene order conservation cannot be systematically attributed to functional constraints, which raises the possibility that other mechanisms can be in place and therefore the underpinnings of the maintenance of gene organization might be more diverse than previously thought.; Why gene order is conserved over long evolutionary timespans remains elusive. A common interpretation is that gene order conservation might reflect the existence of functional constraints that are important for organismal performance. Alteration of the integrity of genomic regions, and therefore of those constraints, would result in detrimental effects. This notion seems especially plausible in those genomes that can easily accommodate gene reshuffling via chromosomal inversions since genomic regions free of constraints are likely to have been disrupted in one or more lineages. Nevertheless, no empirical test has been performed to this notion. Here, we disrupt one of the largest conserved genomic regions of the Drosophila genome by chromosome engineering and examine the phenotypic consequences derived from such disruption. The targeted region exhibits multiple patterns of functional enrichment suggestive of the presence of constraints. The carriers of the disrupted collinear block show no defects in their viability, fertility, and parameters of general homeostasis, although their odorant perception is altered. This change in odorant perception does not correlate with modifications of the level of expression and sex bias of the genes within the genomic region disrupted. Our results indicate that even in highly rearranged genomes, like those of Diptera, unusually high levels of gene order conservation cannot be systematically attributed to functional constraints, which raises the possibility that other mechanisms can be in place and therefore the underpinnings of the maintenance of gene organization might be more diverse than previously thought.
SubtypeArticle
Department[Diaz-Castillo, Carlos; Ranz, Jose M.] Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA; [Xia, Xiao-Qin] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
DOI10.1371/journal.pgen.1002475
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNSF[DEB-0949365] ; NSF[DEB-0949365] ; NSF[DEB-0949365] ; NSF[DEB-0949365]
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000300725500006
WOS KeywordCONSERVED NONCODING SEQUENCES ; GLOBAL CONTROL REGION ; P-ELEMENT INSERTIONS ; MICROARRAY DATA ; CHROMOSOMAL REARRANGEMENT ; REGULATORY LANDSCAPES ; MELANOGASTER GENOME ; GENETIC-VARIABILITY ; ANOPHELES-GAMBIAE ; HOXD CLUSTER
Funding OrganizationNSF[DEB-0949365] ; NSF[DEB-0949365] ; NSF[DEB-0949365] ; NSF[DEB-0949365]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16761
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorDiaz-Castillo, C (reprint author), Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA
Affiliation1.Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Diaz-Castillo, Carlos,Xia, Xiao-Qin,Ranz, Jose M.,et al. Evaluation of the Role of Functional Constraints on the Integrity of an Ultraconserved Region in the Genus Drosophila[J]. PLOS GENETICS,2012,8(2):e1002475.
APA Diaz-Castillo, Carlos,Xia, Xiao-Qin,Ranz, Jose M.,&Diaz-Castillo, C .(2012).Evaluation of the Role of Functional Constraints on the Integrity of an Ultraconserved Region in the Genus Drosophila.PLOS GENETICS,8(2),e1002475.
MLA Diaz-Castillo, Carlos,et al."Evaluation of the Role of Functional Constraints on the Integrity of an Ultraconserved Region in the Genus Drosophila".PLOS GENETICS 8.2(2012):e1002475.
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