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题名: Evaluation of the Role of Functional Constraints on the Integrity of an Ultraconserved Region in the Genus Drosophila
作者: Diaz-Castillo, Carlos1; Xia, Xiao-Qin2; Ranz, Jose M.1
通讯作者: Diaz-Castillo, C (reprint author), Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA
刊名: PLOS GENETICS
发表日期: 2012-02-01
DOI: 10.1371/journal.pgen.1002475
卷: 8, 期:2, 页:e1002475
收录类别: SCI
文章类型: Article
部门归属: [Diaz-Castillo, Carlos; Ranz, Jose M.] Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA; [Xia, Xiao-Qin] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: NSF[DEB-0949365]
类目[WOS]: Genetics & Heredity
研究领域[WOS]: Genetics & Heredity
摘要: Why gene order is conserved over long evolutionary timespans remains elusive. A common interpretation is that gene order conservation might reflect the existence of functional constraints that are important for organismal performance. Alteration of the integrity of genomic regions, and therefore of those constraints, would result in detrimental effects. This notion seems especially plausible in those genomes that can easily accommodate gene reshuffling via chromosomal inversions since genomic regions free of constraints are likely to have been disrupted in one or more lineages. Nevertheless, no empirical test has been performed to this notion. Here, we disrupt one of the largest conserved genomic regions of the Drosophila genome by chromosome engineering and examine the phenotypic consequences derived from such disruption. The targeted region exhibits multiple patterns of functional enrichment suggestive of the presence of constraints. The carriers of the disrupted collinear block show no defects in their viability, fertility, and parameters of general homeostasis, although their odorant perception is altered. This change in odorant perception does not correlate with modifications of the level of expression and sex bias of the genes within the genomic region disrupted. Our results indicate that even in highly rearranged genomes, like those of Diptera, unusually high levels of gene order conservation cannot be systematically attributed to functional constraints, which raises the possibility that other mechanisms can be in place and therefore the underpinnings of the maintenance of gene organization might be more diverse than previously thought.
英文摘要: Why gene order is conserved over long evolutionary timespans remains elusive. A common interpretation is that gene order conservation might reflect the existence of functional constraints that are important for organismal performance. Alteration of the integrity of genomic regions, and therefore of those constraints, would result in detrimental effects. This notion seems especially plausible in those genomes that can easily accommodate gene reshuffling via chromosomal inversions since genomic regions free of constraints are likely to have been disrupted in one or more lineages. Nevertheless, no empirical test has been performed to this notion. Here, we disrupt one of the largest conserved genomic regions of the Drosophila genome by chromosome engineering and examine the phenotypic consequences derived from such disruption. The targeted region exhibits multiple patterns of functional enrichment suggestive of the presence of constraints. The carriers of the disrupted collinear block show no defects in their viability, fertility, and parameters of general homeostasis, although their odorant perception is altered. This change in odorant perception does not correlate with modifications of the level of expression and sex bias of the genes within the genomic region disrupted. Our results indicate that even in highly rearranged genomes, like those of Diptera, unusually high levels of gene order conservation cannot be systematically attributed to functional constraints, which raises the possibility that other mechanisms can be in place and therefore the underpinnings of the maintenance of gene organization might be more diverse than previously thought.
关键词[WOS]: CONSERVED NONCODING SEQUENCES ; GLOBAL CONTROL REGION ; P-ELEMENT INSERTIONS ; MICROARRAY DATA ; CHROMOSOMAL REARRANGEMENT ; REGULATORY LANDSCAPES ; MELANOGASTER GENOME ; GENETIC-VARIABILITY ; ANOPHELES-GAMBIAE ; HOXD CLUSTER
语种: 英语
WOS记录号: WOS:000300725500006
ISSN号: 1553-7404
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16761
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China

Recommended Citation:
Diaz-Castillo, Carlos; Xia, Xiao-Qin; Ranz, Jose M..Evaluation of the Role of Functional Constraints on the Integrity of an Ultraconserved Region in the Genus Drosophila,PLOS GENETICS,2012,8(2):e1002475
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