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The response of carbohydrate metabolism to the fluctuation of relative humidity (RH) in the desert soil cyanobacterium Phormidium tenue
Chen, Lanzhou1; Yang, Yan1; Deng, Songdiang1; Xu, Yanhong1; Wang, Gaohong2; Liu, Yongding2; Chen, LZ (reprint author), Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China
2012
Source PublicationEUROPEAN JOURNAL OF SOIL BIOLOGY
ISSN1164-5563
Volume48Pages:11-16
AbstractThe excreting of exopolysaccharides (EPS) is thought as one of the main protection ways for cyanobacteria in desert algal crusts to survive desiccation. But how cyanobacteria adjust their carbohydrate metabolism to survive this stress is not elucidated. In this study, we treated Phormidium tenue, a cyanobacterium isolated from biological soil crusts with the changes of relative humidity (RH) to simulate different levels of desiccation and investigated its carbohydrate metabolism. It was found that photosynthetic activity (Fv/Fm) and cellular total carbohydrates production decreased significantly at low RH. But the production of EPS, reducing sugar, sucrose and the activity of sucrose phosphate synthase (SPS) increased significantly at low RH and reached maximum at 75% RH. Low RH could also cause the enhanced production of reactive oxygen species (ROS) generation, malondialdehyde (MDA) production and DNA strand breaks. However, when pretreated with exogenous 100 mg/L EPS, Fv/Fm and carbohydrate production were improved significantly, while ROS generation, MDA production and DNA strand breaks decreased significantly at various levels of RH in P tenue. These results indicated that P. tenue in arid regions could enhance desiccation tolerance by adjusting the carbohydrate metabolism to eliminate ROS and decrease oxidative damage. (C) 2011 Elsevier Masson SAS. All rights reserved.; The excreting of exopolysaccharides (EPS) is thought as one of the main protection ways for cyanobacteria in desert algal crusts to survive desiccation. But how cyanobacteria adjust their carbohydrate metabolism to survive this stress is not elucidated. In this study, we treated Phormidium tenue, a cyanobacterium isolated from biological soil crusts with the changes of relative humidity (RH) to simulate different levels of desiccation and investigated its carbohydrate metabolism. It was found that photosynthetic activity (Fv/Fm) and cellular total carbohydrates production decreased significantly at low RH. But the production of EPS, reducing sugar, sucrose and the activity of sucrose phosphate synthase (SPS) increased significantly at low RH and reached maximum at 75% RH. Low RH could also cause the enhanced production of reactive oxygen species (ROS) generation, malondialdehyde (MDA) production and DNA strand breaks. However, when pretreated with exogenous 100 mg/L EPS, Fv/Fm and carbohydrate production were improved significantly, while ROS generation, MDA production and DNA strand breaks decreased significantly at various levels of RH in P tenue. These results indicated that P. tenue in arid regions could enhance desiccation tolerance by adjusting the carbohydrate metabolism to eliminate ROS and decrease oxidative damage. (C) 2011 Elsevier Masson SAS. All rights reserved.
SubtypeArticle
KeywordBiological Soil Crusts Exoplysaccharides (Eps) Desiccation Oxidative Damage
Department[Chen, Lanzhou; Yang, Yan; Deng, Songdiang; Xu, Yanhong] Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China; [Wang, Gaohong; Liu, Yongding] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
DOI10.1016/j.ejsobi.2011.10.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organizationfoundation of Plan of Wuhan Scientific Chenguang of Young People[200850731367]; National Natural Science Foundation of China[30970446, 30500068, 30970688 ; foundation of Plan of Wuhan Scientific Chenguang of Young People[200850731367]; National Natural Science Foundation of China[30970446, 30500068, 30970688 ; foundation of Plan of Wuhan Scientific Chenguang of Young People[200850731367]; National Natural Science Foundation of China[30970446, 30500068, 30970688 ; foundation of Plan of Wuhan Scientific Chenguang of Young People[200850731367]; National Natural Science Foundation of China[30970446, 30500068, 30970688
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Agriculture
WOS SubjectEcology ; Soil Science
WOS IDWOS:000300036500002
WOS KeywordDESICCATION TOLERANCE ; MICROCOLEUS-VAGINATUS ; OXIDATIVE DAMAGE ; SALT STRESS ; DNA-DAMAGE ; EXOPOLYSACCHARIDES ; CRUSTS ; POLYSACCHARIDES ; PHOTOSYNTHESIS ; INVOLVEMENT
Funding Organizationfoundation of Plan of Wuhan Scientific Chenguang of Young People[200850731367]; National Natural Science Foundation of China[30970446, 30500068, 30970688 ; foundation of Plan of Wuhan Scientific Chenguang of Young People[200850731367]; National Natural Science Foundation of China[30970446, 30500068, 30970688 ; foundation of Plan of Wuhan Scientific Chenguang of Young People[200850731367]; National Natural Science Foundation of China[30970446, 30500068, 30970688 ; foundation of Plan of Wuhan Scientific Chenguang of Young People[200850731367]; National Natural Science Foundation of China[30970446, 30500068, 30970688
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16757
Collection水环境工程研究中心_期刊论文
Corresponding AuthorChen, LZ (reprint author), Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China
Affiliation1.Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen, Lanzhou,Yang, Yan,Deng, Songdiang,et al. The response of carbohydrate metabolism to the fluctuation of relative humidity (RH) in the desert soil cyanobacterium Phormidium tenue[J]. EUROPEAN JOURNAL OF SOIL BIOLOGY,2012,48:11-16.
APA Chen, Lanzhou.,Yang, Yan.,Deng, Songdiang.,Xu, Yanhong.,Wang, Gaohong.,...&Chen, LZ .(2012).The response of carbohydrate metabolism to the fluctuation of relative humidity (RH) in the desert soil cyanobacterium Phormidium tenue.EUROPEAN JOURNAL OF SOIL BIOLOGY,48,11-16.
MLA Chen, Lanzhou,et al."The response of carbohydrate metabolism to the fluctuation of relative humidity (RH) in the desert soil cyanobacterium Phormidium tenue".EUROPEAN JOURNAL OF SOIL BIOLOGY 48(2012):11-16.
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