IHB OpenIR  > 水环境工程研究中心  > 期刊论文
Optimal strategies for determination of free/extractable and total microcystins in lake sediment
Wu, Xingqiang2; Wang, Chunbo1,3; Xiao, Bangding1; Wang, Yang2; Zheng, Na2; Liu, Jingshuang2; Xiao, BD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2012-01-04
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
Volume709Issue:0Pages:66-72
AbstractThe optimization of analytical procedures for the quantification of free and total microcystins (MCs) in natural sediments was systematically examined based on solvent extraction and Lemieux oxidation. In this optimized analytical procedure, a sequential solvent extraction using 50% (v/v) methanol and EDTA-sodium pyrophosphate was selected as the optimal extraction solvent for free MCs analysis, after which the purified extracts and sediment residuals were applied to the optimized Lemieux oxidation for determination of total MCs in lake sediments. The optimized procedures were shown to be efficient and reliable for the routine analysis of both free and total MCs in lake sediment samples, as indicated by the minimal adverse impact of sediment organic matter on the recovery of free MCs and yield of MMPB (2-methyl-3-methoxy-4-phenylbutyric acid). Finally, the developed procedures were applied to field sediment samples collected from Lake Dianchi during a bloom season and seven of thirty samples showed positive results. (C) 2011 Elsevier B.V. All rights reserved.; The optimization of analytical procedures for the quantification of free and total microcystins (MCs) in natural sediments was systematically examined based on solvent extraction and Lemieux oxidation. In this optimized analytical procedure, a sequential solvent extraction using 50% (v/v) methanol and EDTA-sodium pyrophosphate was selected as the optimal extraction solvent for free MCs analysis, after which the purified extracts and sediment residuals were applied to the optimized Lemieux oxidation for determination of total MCs in lake sediments. The optimized procedures were shown to be efficient and reliable for the routine analysis of both free and total MCs in lake sediment samples, as indicated by the minimal adverse impact of sediment organic matter on the recovery of free MCs and yield of MMPB (2-methyl-3-methoxy-4-phenylbutyric acid). Finally, the developed procedures were applied to field sediment samples collected from Lake Dianchi during a bloom season and seven of thirty samples showed positive results. (C) 2011 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMicrocystin 2-methyl-3-methoxy-4-phenylbutyric Acid (Mmpb) Sediment Solvent Extraction Lemieux Oxidation
Department[Wang, Chunbo; Xiao, Bangding] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Wu, Xingqiang; Wang, Yang; Zheng, Na; Liu, Jingshuang] Chinese Acad Sci, NE Inst Geog & Agroecol, Key Lab Wetland Ecol & Environm, Changchun 130012, Peoples R China; [Wang, Chunbo] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
DOI10.1016/j.aca.2011.10.027
WOS HeadingsScience & Technology ; Physical Sciences
Funding OrganizationNational Natural Science Foundation of China[41001326]; National Key Project for Basic Research[2008CB418101]; Chinese Academy of Sciences[KZCX2-YW-QN306] ; National Natural Science Foundation of China[41001326]; National Key Project for Basic Research[2008CB418101]; Chinese Academy of Sciences[KZCX2-YW-QN306] ; National Natural Science Foundation of China[41001326]; National Key Project for Basic Research[2008CB418101]; Chinese Academy of Sciences[KZCX2-YW-QN306] ; National Natural Science Foundation of China[41001326]; National Key Project for Basic Research[2008CB418101]; Chinese Academy of Sciences[KZCX2-YW-QN306]
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000299801500008
WOS KeywordCOVALENTLY BOUND MICROCYSTINS ; LIQUID-CHROMATOGRAPHY ; RISK-ASSESSMENT ; ANIMAL-TISSUES ; LR ; WATER ; CYANOBACTERIA ; EXTRACTION ; BIOACCUMULATION ; OPTIMIZATION
Funding OrganizationNational Natural Science Foundation of China[41001326]; National Key Project for Basic Research[2008CB418101]; Chinese Academy of Sciences[KZCX2-YW-QN306] ; National Natural Science Foundation of China[41001326]; National Key Project for Basic Research[2008CB418101]; Chinese Academy of Sciences[KZCX2-YW-QN306] ; National Natural Science Foundation of China[41001326]; National Key Project for Basic Research[2008CB418101]; Chinese Academy of Sciences[KZCX2-YW-QN306] ; National Natural Science Foundation of China[41001326]; National Key Project for Basic Research[2008CB418101]; Chinese Academy of Sciences[KZCX2-YW-QN306]
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16749
Collection水环境工程研究中心_期刊论文
Corresponding AuthorXiao, BD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, NE Inst Geog & Agroecol, Key Lab Wetland Ecol & Environm, Changchun 130012, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Wu, Xingqiang,Wang, Chunbo,Xiao, Bangding,et al. Optimal strategies for determination of free/extractable and total microcystins in lake sediment[J]. ANALYTICA CHIMICA ACTA,2012,709(0):66-72.
APA Wu, Xingqiang.,Wang, Chunbo.,Xiao, Bangding.,Wang, Yang.,Zheng, Na.,...&Xiao, BD .(2012).Optimal strategies for determination of free/extractable and total microcystins in lake sediment.ANALYTICA CHIMICA ACTA,709(0),66-72.
MLA Wu, Xingqiang,et al."Optimal strategies for determination of free/extractable and total microcystins in lake sediment".ANALYTICA CHIMICA ACTA 709.0(2012):66-72.
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