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Disruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol
Ma, Yanbo1,2; Han, Jian1; Guo, Yongyong1; Lam, Paul K. S.3; Wu, Rudolf S. S.4; Giesy, John P.3,4,5,6,7,8; Zhang, Xiaowei9; Zhou, Bingsheng1; Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2012-01-15
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume106Pages:173-181
AbstractChlorophenols in the aquatic environment have been of concern due to their potential effects on human and wildlife. In the present study, the endocrine disrupting effects of 2,4-dichlorophenol (2,4-DCP) were investigated in vitro and in vivo. In the in vitro assay, H295R human adrenocortical carcinoma cells were used to determine the potential effects of 2,4-DCP on steroidogenesis. Exposure to 0, 0.1, 0.3 or 1.0 mg 2,4-DCP/L resulted in less production of 17 beta-estradiol (E2) and alterations in transcript expressions of genes involved in steroidogenesis, including cytochrome P450 (CYP11A, CYP17, CYP19), 3 beta HSD, 17 beta HSD and StAR. In the in vivo study, effects of 0, 0.03, 0.1 or 0.3 mg 2,4-DCP/L on concentrations of steroid hormones in plasma of adult zebrafish (Danio rerio) were measured and expression of mRNA of selected genes in hypothalamic-pituitary-gonadal (HPG) axis and liver were determined. Exposure of zebrafish to 2,4-DCP resulted in lesser concentrations of E2 accompanied by down-regulation of CYP19A mRNA in the females. In males, exposure to 2,4-DCP resulted in greater concentrations of testosterone (T) and E2 along with greater mRNA expression of CYP17 and CYP19A. The mRNA expression of prostaglandin synthase (Ptgs2) gene, which regulates ovulation, was down-regulated in females, but up-regulated in males. The hepatic estrogenic receptor (ER alpha and ER beta) and vitellogenin (VTG1 and VTG3) mRNAs were up-regulated in both females and males. The average number of eggs spawned was significantly less upon exposure to 2,4-DCP. Exposure of adult zebrafish to 2,4-DCP resulted in lesser rates of hatching of eggs. The results demonstrated that 2,4-DCP modulates transcription of steroidogenetic genes in both H295R cells and in the zebrafish HPG-axis and disrupts steroidogenesis, which in turn, can cause adverse effects on reproduction in fish. (C) 2011 Elsevier B.V. All rights reserved.; Chlorophenols in the aquatic environment have been of concern due to their potential effects on human and wildlife. In the present study, the endocrine disrupting effects of 2,4-dichlorophenol (2,4-DCP) were investigated in vitro and in vivo. In the in vitro assay, H295R human adrenocortical carcinoma cells were used to determine the potential effects of 2,4-DCP on steroidogenesis. Exposure to 0, 0.1, 0.3 or 1.0 mg 2,4-DCP/L resulted in less production of 17 beta-estradiol (E2) and alterations in transcript expressions of genes involved in steroidogenesis, including cytochrome P450 (CYP11A, CYP17, CYP19), 3 beta HSD, 17 beta HSD and StAR. In the in vivo study, effects of 0, 0.03, 0.1 or 0.3 mg 2,4-DCP/L on concentrations of steroid hormones in plasma of adult zebrafish (Danio rerio) were measured and expression of mRNA of selected genes in hypothalamic-pituitary-gonadal (HPG) axis and liver were determined. Exposure of zebrafish to 2,4-DCP resulted in lesser concentrations of E2 accompanied by down-regulation of CYP19A mRNA in the females. In males, exposure to 2,4-DCP resulted in greater concentrations of testosterone (T) and E2 along with greater mRNA expression of CYP17 and CYP19A. The mRNA expression of prostaglandin synthase (Ptgs2) gene, which regulates ovulation, was down-regulated in females, but up-regulated in males. The hepatic estrogenic receptor (ER alpha and ER beta) and vitellogenin (VTG1 and VTG3) mRNAs were up-regulated in both females and males. The average number of eggs spawned was significantly less upon exposure to 2,4-DCP. Exposure of adult zebrafish to 2,4-DCP resulted in lesser rates of hatching of eggs. The results demonstrated that 2,4-DCP modulates transcription of steroidogenetic genes in both H295R cells and in the zebrafish HPG-axis and disrupts steroidogenesis, which in turn, can cause adverse effects on reproduction in fish. (C) 2011 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordHormones In Vitro In Vivo H295r Hpg Axis Zebrafish
Department[Ma, Yanbo; Han, Jian; Guo, Yongyong; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Ma, Yanbo] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Lam, Paul K. S.; Giesy, John P.] City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China; [Wu, Rudolf S. S.; Giesy, John P.] Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China; [Giesy, John P.] Univ Saskatchewan, Dept Vet, Saskatoon, SK, Canada; [Giesy, John P.] Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK, Canada; [Giesy, John P.] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK, Canada; [Giesy, John P.] King Saud Univ, Dept Zool, Coll Sci, Riyadh 11451, Saudi Arabia; [Zhang, Xiaowei] Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing, Jiangsu, Peoples R China
DOI10.1016/j.aquatox.2011.11.006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationChinese Academy of Sciences[KZCX2-YW-Q02-05]; National Nature Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ10]; National Science and Engineering Research Council of Canada[32641507]; Western Economic Diversification Canada[6578, 6807]; Canada Research Chair program; Department of Biology and Chemistry; State Key Laboratory in Marine Pollution, City University of Hong Kong; Chinese Academy of Sciences; King Saud University ; Chinese Academy of Sciences[KZCX2-YW-Q02-05]; National Nature Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ10]; National Science and Engineering Research Council of Canada[32641507]; Western Economic Diversification Canada[6578, 6807]; Canada Research Chair program; Department of Biology and Chemistry; State Key Laboratory in Marine Pollution, City University of Hong Kong; Chinese Academy of Sciences; King Saud University ; Chinese Academy of Sciences[KZCX2-YW-Q02-05]; National Nature Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ10]; National Science and Engineering Research Council of Canada[32641507]; Western Economic Diversification Canada[6578, 6807]; Canada Research Chair program; Department of Biology and Chemistry; State Key Laboratory in Marine Pollution, City University of Hong Kong; Chinese Academy of Sciences; King Saud University ; Chinese Academy of Sciences[KZCX2-YW-Q02-05]; National Nature Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ10]; National Science and Engineering Research Council of Canada[32641507]; Western Economic Diversification Canada[6578, 6807]; Canada Research Chair program; Department of Biology and Chemistry; State Key Laboratory in Marine Pollution, City University of Hong Kong; Chinese Academy of Sciences; King Saud University
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000300205300021
WOS KeywordPITUITARY-GONADAL AXIS ; REAL-TIME PCR ; GENE-EXPRESSION ; OOCYTE MATURATION ; HORMONE PRODUCTION ; ORYZIAS-LATIPES ; AROMATASE GENES ; RAINBOW-TROUT ; STEROIDOGENESIS ; FISH
Funding OrganizationChinese Academy of Sciences[KZCX2-YW-Q02-05]; National Nature Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ10]; National Science and Engineering Research Council of Canada[32641507]; Western Economic Diversification Canada[6578, 6807]; Canada Research Chair program; Department of Biology and Chemistry; State Key Laboratory in Marine Pollution, City University of Hong Kong; Chinese Academy of Sciences; King Saud University ; Chinese Academy of Sciences[KZCX2-YW-Q02-05]; National Nature Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ10]; National Science and Engineering Research Council of Canada[32641507]; Western Economic Diversification Canada[6578, 6807]; Canada Research Chair program; Department of Biology and Chemistry; State Key Laboratory in Marine Pollution, City University of Hong Kong; Chinese Academy of Sciences; King Saud University ; Chinese Academy of Sciences[KZCX2-YW-Q02-05]; National Nature Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ10]; National Science and Engineering Research Council of Canada[32641507]; Western Economic Diversification Canada[6578, 6807]; Canada Research Chair program; Department of Biology and Chemistry; State Key Laboratory in Marine Pollution, City University of Hong Kong; Chinese Academy of Sciences; King Saud University ; Chinese Academy of Sciences[KZCX2-YW-Q02-05]; National Nature Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ10]; National Science and Engineering Research Council of Canada[32641507]; Western Economic Diversification Canada[6578, 6807]; Canada Research Chair program; Department of Biology and Chemistry; State Key Laboratory in Marine Pollution, City University of Hong Kong; Chinese Academy of Sciences; King Saud University
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16743
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China
4.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
5.Univ Saskatchewan, Dept Vet, Saskatoon, SK, Canada
6.Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK, Canada
7.Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK, Canada
8.King Saud Univ, Dept Zool, Coll Sci, Riyadh 11451, Saudi Arabia
9.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Ma, Yanbo,Han, Jian,Guo, Yongyong,et al. Disruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol[J]. AQUATIC TOXICOLOGY,2012,106:173-181.
APA Ma, Yanbo.,Han, Jian.,Guo, Yongyong.,Lam, Paul K. S..,Wu, Rudolf S. S..,...&Zhou, BS .(2012).Disruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol.AQUATIC TOXICOLOGY,106,173-181.
MLA Ma, Yanbo,et al."Disruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol".AQUATIC TOXICOLOGY 106(2012):173-181.
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