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题名: Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare
作者: Zhang Jin1, 2; Zou Hong1; Wang Liangfa1; Huang Bei1; Li Nan1; Wang Guitang1; Nie Pin1
通讯作者: Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Flavobacterium columnare ; selectable marker ; integrative/conjugative plasmid ; gene disruption
刊名: CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
发表日期: 2012-03-01
DOI: 10.1007/s00343-012-1077-z
卷: 30, 期:2, 页:269-278
收录类别: SCI
文章类型: Article
部门归属: [Zhang Jin; Zou Hong; Wang Liangfa; Huang Bei; Li Nan; Wang Guitang; Nie Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者: Chinese Ministry of Science and Technology[2011CB944600]; US Department of Energy[DE-FG02-02ER15332]; Fudan University; Rijk Zwaan; Institute of Hydrobiology; Biology Department; Huck Institutes of the Life Sciences at the Pennsylvania State University
类目[WOS]: Limnology ; Oceanography
研究领域[WOS]: Marine & Freshwater Biology ; Oceanography
摘要: Flavobacterium columnare, the etiological agent of columnaris disease, is one of the most important and widespread bacterial pathogens of freshwater fish. In this study, we constructed two artificial selectable markers (chloramphenicol and spectinomycin resistance) for gene transfer in F. columnare. These two new artificial selectable markers, which were created by placing the chloramphenicol or spectinomycin resistance gene under the control of the native acs regulatory region of F. columnare, were functional in both F. columnare and Escherichia coli. The integrative/conjugative plasmids constructed by using these markers were introduced into F. columnare G(4) via electroporation or conjugation. The integrated plasmid DNA was confirmed by Southern blotting and PCR analysis. These two markers can be employed in future investigations into gene deletion and the pathogenicity of virulence factors in F. columnare.
英文摘要: Flavobacterium columnare, the etiological agent of columnaris disease, is one of the most important and widespread bacterial pathogens of freshwater fish. In this study, we constructed two artificial selectable markers (chloramphenicol and spectinomycin resistance) for gene transfer in F. columnare. These two new artificial selectable markers, which were created by placing the chloramphenicol or spectinomycin resistance gene under the control of the native acs regulatory region of F. columnare, were functional in both F. columnare and Escherichia coli. The integrative/conjugative plasmids constructed by using these markers were introduced into F. columnare G(4) via electroporation or conjugation. The integrated plasmid DNA was confirmed by Southern blotting and PCR analysis. These two markers can be employed in future investigations into gene deletion and the pathogenicity of virulence factors in F. columnare.
关键词[WOS]: CHONDROITIN AC LYASE ; CATFISH ICTALURUS-PUNCTATUS ; GLIDING MOTILITY ; CHANNEL CATFISH ; FLEXIBACTER-COLUMNARIS ; GENETIC MANIPULATION ; BACTEROIDES-FRAGILIS ; CYTOPHAGA JOHNSONAE ; ESCHERICHIA-COLI ; LOW VIRULENCE
语种: 英语
WOS记录号: WOS:000301001900011
ISSN号: 0254-4059
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16741
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Zhang Jin; Zou Hong; Wang Liangfa; Huang Bei; Li Nan; Wang Guitang; Nie Pin.Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare,CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(2):269-278
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文件名: Construction of two selectable markers for integrative conjugative plasmids in Flavobacterium columnare.pdf
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