IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome
Gao, E-Bin; Gui, Jian-Fang; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2012
Source PublicationJOURNAL OF VIROLOGY
ISSN0022-538X
Volume86Issue:1Pages:236-245
AbstractA cyanophage, PaV-LD, has been isolated from harmful filamentous cyanobacterium Planktothrix agardhii in Lake Donghu, a shallow freshwater lake in China. Here, we present the cyanophage's genomic organization and major structural proteins. The genome is a 95,299-bp-long, linear double-stranded DNA and contains 142 potential genes. BLAST searches revealed 29 proteins of known function in cyanophages, cyanobacteria, or bacteria. Thirteen major structural proteins ranging in size from 27 kDa to 172 kDa were identified by SDS-PAGE and mass-spectrometric analysis. The genome lacks major genes that are necessary to the tail structure, and the tailless PaV-LD has been confirmed by an electron microscopy comparison with other tail cyanophages and phages. Phylogenetic analysis of the major capsid proteins also reveals an independent branch of PaV-LD that is quite different from other known tail cyanophages and phages. Moreover, the unique genome carries a nonbleaching protein A (NblA) gene (open reading frame [ORF] 022L), which is present in all phycobilisome-containing organisms and mediates phycobilisome degradation. Western blot detection confirmed that 022L was expressed after PaV-LD infection in the host filamentous cyanobacterium. In addition, its appearance was companied by a significant decline of phycocyanobilin content and a color change of the cyanobacterial cells from blue-green to yellow-green. The biological function of PaV-LD nblA was further confirmed by expression in a model cyanobacterium via an integration platform, by spectroscopic analysis and electron microscopy observation. The data indicate that PaV-LD is an exceptional cyanophage of filamentous cyanobacteria, and this novel cyanophage will also provide us with a new vision of the cyanophage-host interactions.; A cyanophage, PaV-LD, has been isolated from harmful filamentous cyanobacterium Planktothrix agardhii in Lake Donghu, a shallow freshwater lake in China. Here, we present the cyanophage's genomic organization and major structural proteins. The genome is a 95,299-bp-long, linear double-stranded DNA and contains 142 potential genes. BLAST searches revealed 29 proteins of known function in cyanophages, cyanobacteria, or bacteria. Thirteen major structural proteins ranging in size from 27 kDa to 172 kDa were identified by SDS-PAGE and mass-spectrometric analysis. The genome lacks major genes that are necessary to the tail structure, and the tailless PaV-LD has been confirmed by an electron microscopy comparison with other tail cyanophages and phages. Phylogenetic analysis of the major capsid proteins also reveals an independent branch of PaV-LD that is quite different from other known tail cyanophages and phages. Moreover, the unique genome carries a nonbleaching protein A (NblA) gene (open reading frame [ORF] 022L), which is present in all phycobilisome-containing organisms and mediates phycobilisome degradation. Western blot detection confirmed that 022L was expressed after PaV-LD infection in the host filamentous cyanobacterium. In addition, its appearance was companied by a significant decline of phycocyanobilin content and a color change of the cyanobacterial cells from blue-green to yellow-green. The biological function of PaV-LD nblA was further confirmed by expression in a model cyanobacterium via an integration platform, by spectroscopic analysis and electron microscopy observation. The data indicate that PaV-LD is an exceptional cyanophage of filamentous cyanobacteria, and this novel cyanophage will also provide us with a new vision of the cyanophage-host interactions.
SubtypeArticle
Department[Gao, E-Bin; Gui, Jian-Fang; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1128/JVI.06282-11
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program[2010CB126303, 2009CB118704]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2010CB126303, 2009CB118704]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2010CB126303, 2009CB118704]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2010CB126303, 2009CB118704]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16]
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000298347700023
WOS KeywordLACTOCOCCAL BACTERIOPHAGE TP901-1 ; MARINE SYNECHOCOCCUS STRAINS ; MYCOBACTERIOPHAGE GENOMES ; PHOTOSYNTHESIS GENES ; EFFICIENT INFECTION ; TAIL PROTEINS ; PHAGE ; PROCHLOROCOCCUS ; SEQUENCE ; DIVERSITY
Funding OrganizationNational Major Basic Research Program[2010CB126303, 2009CB118704]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2010CB126303, 2009CB118704]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2010CB126303, 2009CB118704]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2010CB126303, 2009CB118704]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16]
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16739
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Gao, E-Bin,Gui, Jian-Fang,Zhang, Qi-Ya,et al. A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome[J]. JOURNAL OF VIROLOGY,2012,86(1):236-245.
APA Gao, E-Bin,Gui, Jian-Fang,Zhang, Qi-Ya,&Zhang, QY .(2012).A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome.JOURNAL OF VIROLOGY,86(1),236-245.
MLA Gao, E-Bin,et al."A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome".JOURNAL OF VIROLOGY 86.1(2012):236-245.
Files in This Item: Download All
File Name/Size DocType Version Access License
A Novel Cyanophage w(942KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Gao, E-Bin]'s Articles
[Gui, Jian-Fang]'s Articles
[Zhang, Qi-Ya]'s Articles
Baidu academic
Similar articles in Baidu academic
[Gao, E-Bin]'s Articles
[Gui, Jian-Fang]'s Articles
[Zhang, Qi-Ya]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Gao, E-Bin]'s Articles
[Gui, Jian-Fang]'s Articles
[Zhang, Qi-Ya]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.