中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
题名: Three goose-type lysozymes in the gastropod Oncomelania hupensis: cDNA sequences and lytic activity of recombinant proteins
作者: Zhang, Shu H.1; Zhu, Dan D.1; Chang, Ming X.1; Zhao, Qin P.2; Jiao, Ran1; Huang, Bei1; Fu, Jian P.1; Liu, Zhi X.1; Nie, P.1
通讯作者: Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
关键词: Goose-type lysozyme ; Lytic activity ; Recombinant protein ; Oncomelania hupensis ; Mollusc
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2012
DOI: 10.1016/j.dci.2011.06.014
卷: 36, 期:1, 页:241-246
收录类别: SCI
文章类型: Article
部门归属: [Zhang, Shu H.; Zhu, Dan D.; Chang, Ming X.; Jiao, Ran; Huang, Bei; Fu, Jian P.; Liu, Zhi X.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Zhao, Qin P.] Wuhan Univ, Sch Basic Med Sci, Dept Parasitol, Wuhan 430071, Hubei Province, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Chinese Academy of Sciences[KSCX2-YW-N-055]; National Natural Science Foundation of China[30770353]
类目[WOS]: Immunology ; Zoology
研究领域[WOS]: Immunology ; Zoology
摘要: Three goose-type (g-type) lysozymes, designated as OHLysG1, OHLysG2 and OHLysG3 were identified from expressed sequence tags (ESTs) of a gastropod Oncomelania hupensis, the intermediate host of Schistosoma japonicum. The full cDNA sequences of OHLysG1, OHLysG2 and OHLysG3 consisted of 735, 909 and 808 nucleotides, with an open reading frame of 198, 214 and 249 codons containing a 21, 7 and 8 amino acid (aa) signal peptide at the N-terminus, respectively. The three g-type lysozymes shared conserved features with other g-type lysozymes, such as the substrate binding sites, the catalytic residues critical for the fundamental structure and function of g-type lysozymes. It seems possible that g-type lysozymes in molluscs shared one conserved cysteine with those in birds and mammals, and six conserved cysteines were observed for mollusc g-type lysozymes, with two unique cysteines present in the g-type lysozymes of O. hupensis. The three lysozyme genes were expressed mainly in hepatopancreas, with relatively low expression level observed in head-foot muscle and intestine. When comparing S. japonicum-infected and uninfected snails, significant increase (P < 0.05) was observed for all the three lysozymes in infected snails, with the highest increase detected in hepatopancreas, and lowest in intestine, implying their defensive role in the host-parasite, i.e. snail-trematode system. The three recombinant lysozymes expressed in Escherichia coil strain M15 showed lytic activity against Aeromonas hydrophila, Vibrio fluvialis, Aeromonas sobria and Micrococcus lysodeikticus. In conclusion, the finding of three g-type lysozymes in O. hupensis provides structural and functional evidence of multiple g-type lysozymes in gastropod, which may have evolutional implication in the snail-trematode system. (C) 2011 Elsevier Ltd. All rights reserved.
英文摘要: Three goose-type (g-type) lysozymes, designated as OHLysG1, OHLysG2 and OHLysG3 were identified from expressed sequence tags (ESTs) of a gastropod Oncomelania hupensis, the intermediate host of Schistosoma japonicum. The full cDNA sequences of OHLysG1, OHLysG2 and OHLysG3 consisted of 735, 909 and 808 nucleotides, with an open reading frame of 198, 214 and 249 codons containing a 21, 7 and 8 amino acid (aa) signal peptide at the N-terminus, respectively. The three g-type lysozymes shared conserved features with other g-type lysozymes, such as the substrate binding sites, the catalytic residues critical for the fundamental structure and function of g-type lysozymes. It seems possible that g-type lysozymes in molluscs shared one conserved cysteine with those in birds and mammals, and six conserved cysteines were observed for mollusc g-type lysozymes, with two unique cysteines present in the g-type lysozymes of O. hupensis. The three lysozyme genes were expressed mainly in hepatopancreas, with relatively low expression level observed in head-foot muscle and intestine. When comparing S. japonicum-infected and uninfected snails, significant increase (P < 0.05) was observed for all the three lysozymes in infected snails, with the highest increase detected in hepatopancreas, and lowest in intestine, implying their defensive role in the host-parasite, i.e. snail-trematode system. The three recombinant lysozymes expressed in Escherichia coli strain M15 showed lytic activity against Aeromonas hydrophila, Vibrio fluvialis, Aeromonas sobria and Micrococcus lysodeikticus. In conclusion, the finding of three g-type lysozymes in O. hupensis provides structural and functional evidence of multiple g-type lysozymes in gastropod, which may have evolutional implication in the snail-trematode system. (C) 2011 Elsevier Ltd. All rights reserved.
关键词[WOS]: MOLECULAR-CLONING ; CHLAMYS-FARRERI ; CHICKEN-TYPE ; EGG-WHITE ; C-TYPE ; GENE ; EXPRESSION ; EVOLUTION ; ORGANIZATION ; MOLLUSKS
语种: 英语
WOS记录号: WOS:000299390300028
ISSN号: 0145-305X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16737
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Three goose-type lysozymes in the gastropod Oncomelania hupensis cDNA sequences and lytic activity of recombinant proteins.pdf(372KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Wuhan Univ, Sch Basic Med Sci, Dept Parasitol, Wuhan 430071, Hubei Province, Peoples R China

Recommended Citation:
Zhang, Shu H.; Zhu, Dan D.; Chang, Ming X.; Zhao, Qin P.; Jiao, Ran; Huang, Bei; Fu, Jian P.; Liu, Zhi X.; Nie, P..Three goose-type lysozymes in the gastropod Oncomelania hupensis: cDNA sequences and lytic activity of recombinant proteins,DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,36(1):241-246
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Shu H.]'s Articles
[Zhu, Dan D.]'s Articles
[Chang, Ming X.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Shu H.]‘s Articles
[Zhu, Dan D.]‘s Articles
[Chang, Ming X.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Three goose-type lysozymes in the gastropod Oncomelania hupensis cDNA sequences and lytic activity of recombinant proteins.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace