IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2
Wang, Xiaohu1; Zhang, Yibing1,2; Yang, Xuexian O.1; Nurieva, Roza I.1; Chang, Seon Hee1; Ojeda, Sandra S.1; Kang, Hong S.3; Schluns, Kimberly S.1; Gui, Jianfang2; Jetten, Anton M.3; Dong, Chen1; Dong, C (reprint author), Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA
2012-01-27
Source PublicationIMMUNITY
ISSN1074-7613
Volume36Issue:1Pages:23-31
AbstractT helper 17 (Th17) cells specifically transcribe the Il17 and Il17f genes, which are localized in the same chromosome region, but the underlying mechanism is unclear. Here, we report a cis element that we previously named conserved noncoding sequence 2 (CNS2) physically interacted with both Il17 and Il17f gene promoters and was sufficient for regulating their selective transcription in Th17 cells. Targeted deletion of CNS2 resulted in impaired retinoic acid-related orphan receptor gammat (ROR gamma t)-driven IL-17 expression in vitro. CNS2-deficient T cells also produced substantially decreased amounts of IL-17F. These cytokine defects were associated with defective chromatin remodeling in the Ild17-Il17f gene locus, possibly because of effects on CNS2-mediated recruitment of histone-modifying enzymes p300 and JmjC domain-containing protein 3 (JMJD3). CNS2-deficient animals were also shown to be resistant to experimental autoimmune encephalomyelitis (EAE). Our results thus suggest that CNS2 is sufficient and necessary for Il17 and optimal Il17f gene transcription in Th17 cells.; T helper 17 (Th17) cells specifically transcribe the Il17 and Il17f genes, which are localized in the same chromosome region, but the underlying mechanism is unclear. Here, we report a cis element that we previously named conserved noncoding sequence 2 (CNS2) physically interacted with both Il17 and Il17f gene promoters and was sufficient for regulating their selective transcription in Th17 cells. Targeted deletion of CNS2 resulted in impaired retinoic acid-related orphan receptor gammat (ROR gamma t)-driven IL-17 expression in vitro. CNS2-deficient T cells also produced substantially decreased amounts of IL-17F. These cytokine defects were associated with defective chromatin remodeling in the Ild17-Il17f gene locus, possibly because of effects on CNS2-mediated recruitment of histone-modifying enzymes p300 and JmjC domain-containing protein 3 (JMJD3). CNS2-deficient animals were also shown to be resistant to experimental autoimmune encephalomyelitis (EAE). Our results thus suggest that CNS2 is sufficient and necessary for Il17 and optimal Il17f gene transcription in Th17 cells.
SubtypeArticle
Department[Wang, Xiaohu; Zhang, Yibing; Yang, Xuexian O.; Nurieva, Roza I.; Chang, Seon Hee; Ojeda, Sandra S.; Schluns, Kimberly S.; Dong, Chen] Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA; [Zhang, Yibing; Gui, Jianfang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Kang, Hong S.; Jetten, Anton M.] NIEHS, Cell Biol Sect, LRB, NIH, Res Triangle Pk, NC 27709 USA
DOI10.1016/j.immuni.2011.10.019
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNIH; American Heart Association; 973 National Basic Research Program of China[2010CB126301]; National Multiple Sclerosis Society; Chinese Academy of Sciences; MD Anderson Cancer Center; Leukemia and Lymphoma Society ; NIH; American Heart Association; 973 National Basic Research Program of China[2010CB126301]; National Multiple Sclerosis Society; Chinese Academy of Sciences; MD Anderson Cancer Center; Leukemia and Lymphoma Society ; NIH; American Heart Association; 973 National Basic Research Program of China[2010CB126301]; National Multiple Sclerosis Society; Chinese Academy of Sciences; MD Anderson Cancer Center; Leukemia and Lymphoma Society ; NIH; American Heart Association; 973 National Basic Research Program of China[2010CB126301]; National Multiple Sclerosis Society; Chinese Academy of Sciences; MD Anderson Cancer Center; Leukemia and Lymphoma Society
Indexed BySCI
Language英语
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000299766000007
WOS KeywordEXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS ; RANGE INTRACHROMOSOMAL INTERACTIONS ; CD4(+) T-CELLS ; ROR-GAMMA-T ; NUCLEAR RECEPTORS ; GENE-EXPRESSION ; DIFFERENTIATION ; CYTOKINE ; LOCUS ; LINEAGE
Funding OrganizationNIH; American Heart Association; 973 National Basic Research Program of China[2010CB126301]; National Multiple Sclerosis Society; Chinese Academy of Sciences; MD Anderson Cancer Center; Leukemia and Lymphoma Society ; NIH; American Heart Association; 973 National Basic Research Program of China[2010CB126301]; National Multiple Sclerosis Society; Chinese Academy of Sciences; MD Anderson Cancer Center; Leukemia and Lymphoma Society ; NIH; American Heart Association; 973 National Basic Research Program of China[2010CB126301]; National Multiple Sclerosis Society; Chinese Academy of Sciences; MD Anderson Cancer Center; Leukemia and Lymphoma Society ; NIH; American Heart Association; 973 National Basic Research Program of China[2010CB126301]; National Multiple Sclerosis Society; Chinese Academy of Sciences; MD Anderson Cancer Center; Leukemia and Lymphoma Society
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Cited Times:62[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16731
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorDong, C (reprint author), Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA
Affiliation1.Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.NIEHS, Cell Biol Sect, LRB, NIH, Res Triangle Pk, NC 27709 USA
Recommended Citation
GB/T 7714
Wang, Xiaohu,Zhang, Yibing,Yang, Xuexian O.,et al. Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2[J]. IMMUNITY,2012,36(1):23-31.
APA Wang, Xiaohu.,Zhang, Yibing.,Yang, Xuexian O..,Nurieva, Roza I..,Chang, Seon Hee.,...&Dong, C .(2012).Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2.IMMUNITY,36(1),23-31.
MLA Wang, Xiaohu,et al."Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2".IMMUNITY 36.1(2012):23-31.
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