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题名: Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2
作者: Wang, Xiaohu1; Zhang, Yibing1, 2; Yang, Xuexian O.1; Nurieva, Roza I.1; Chang, Seon Hee1; Ojeda, Sandra S.1; Kang, Hong S.3; Schluns, Kimberly S.1; Gui, Jianfang2; Jetten, Anton M.3; Dong, Chen1
通讯作者: Dong, C (reprint author), Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA
刊名: IMMUNITY
发表日期: 2012-01-27
DOI: 10.1016/j.immuni.2011.10.019
卷: 36, 期:1, 页:23-31
收录类别: SCI
文章类型: Article
部门归属: [Wang, Xiaohu; Zhang, Yibing; Yang, Xuexian O.; Nurieva, Roza I.; Chang, Seon Hee; Ojeda, Sandra S.; Schluns, Kimberly S.; Dong, Chen] Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA; [Zhang, Yibing; Gui, Jianfang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Kang, Hong S.; Jetten, Anton M.] NIEHS, Cell Biol Sect, LRB, NIH, Res Triangle Pk, NC 27709 USA
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: NIH; American Heart Association; 973 National Basic Research Program of China[2010CB126301]; National Multiple Sclerosis Society; Chinese Academy of Sciences; MD Anderson Cancer Center; Leukemia and Lymphoma Society
类目[WOS]: Immunology
研究领域[WOS]: Immunology
摘要: T helper 17 (Th17) cells specifically transcribe the Il17 and Il17f genes, which are localized in the same chromosome region, but the underlying mechanism is unclear. Here, we report a cis element that we previously named conserved noncoding sequence 2 (CNS2) physically interacted with both Il17 and Il17f gene promoters and was sufficient for regulating their selective transcription in Th17 cells. Targeted deletion of CNS2 resulted in impaired retinoic acid-related orphan receptor gammat (ROR gamma t)-driven IL-17 expression in vitro. CNS2-deficient T cells also produced substantially decreased amounts of IL-17F. These cytokine defects were associated with defective chromatin remodeling in the Ild17-Il17f gene locus, possibly because of effects on CNS2-mediated recruitment of histone-modifying enzymes p300 and JmjC domain-containing protein 3 (JMJD3). CNS2-deficient animals were also shown to be resistant to experimental autoimmune encephalomyelitis (EAE). Our results thus suggest that CNS2 is sufficient and necessary for Il17 and optimal Il17f gene transcription in Th17 cells.
英文摘要: T helper 17 (Th17) cells specifically transcribe the Il17 and Il17f genes, which are localized in the same chromosome region, but the underlying mechanism is unclear. Here, we report a cis element that we previously named conserved noncoding sequence 2 (CNS2) physically interacted with both Il17 and Il17f gene promoters and was sufficient for regulating their selective transcription in Th17 cells. Targeted deletion of CNS2 resulted in impaired retinoic acid-related orphan receptor gammat (ROR gamma t)-driven IL-17 expression in vitro. CNS2-deficient T cells also produced substantially decreased amounts of IL-17F. These cytokine defects were associated with defective chromatin remodeling in the Ild17-Il17f gene locus, possibly because of effects on CNS2-mediated recruitment of histone-modifying enzymes p300 and JmjC domain-containing protein 3 (JMJD3). CNS2-deficient animals were also shown to be resistant to experimental autoimmune encephalomyelitis (EAE). Our results thus suggest that CNS2 is sufficient and necessary for Il17 and optimal Il17f gene transcription in Th17 cells.
关键词[WOS]: EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS ; RANGE INTRACHROMOSOMAL INTERACTIONS ; CD4(+) T-CELLS ; ROR-GAMMA-T ; NUCLEAR RECEPTORS ; GENE-EXPRESSION ; DIFFERENTIATION ; CYTOKINE ; LOCUS ; LINEAGE
语种: 英语
WOS记录号: WOS:000299766000007
ISSN号: 1074-7613
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16731
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.NIEHS, Cell Biol Sect, LRB, NIH, Res Triangle Pk, NC 27709 USA

Recommended Citation:
Wang, Xiaohu; Zhang, Yibing; Yang, Xuexian O.; Nurieva, Roza I.; Chang, Seon Hee; Ojeda, Sandra S.; Kang, Hong S.; Schluns, Kimberly S.; Gui, Jianfang; Jetten, Anton M.; Dong, Chen.Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2,IMMUNITY,2012,36(1):23-31
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文件名: CNS2-sup.pdf
格式: Adobe PDF
文件名: CNS2-paper.pdf
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