IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)
Yuan, Han-wen; Guo, Long-gen; Xu, Qiao-qing; Xu, QQ (reprint author), Yangtze Univ, Engn Res Ctr Wetland Agr Middle Reaches Yangtze R, Minist Educ, Jinzhou 434025, Hubei Province, Peoples R China; xuqiaoqing@163.com
2011
Source PublicationIRANIAN JOURNAL OF FISHERIES SCIENCES
ISSN1562-2916
Volume10Issue:4Pages:631-640
AbstractMicrosatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 primer pairs designed, 25 yielded scorable amplification products. Upon testing 30 individuals were sampled using the 25 pair primers from Dongting Lake of Hunan Province, China. Then thirteen polymorphic microsatellite markers were isolated and characterized. These loci exhibited high levels of genetic polymorphism, so the observed number of alleles per locus ranged from 4 to 9. The ranges of observed and expected heterozygosity were 0.2543 to 0.8913 and 0.3629 to 0.8217, respectively, and the average polymorphic information content was 0.5198. Two microsatellite loci were significantly deviated from Hardy-Weinberg equilibrium due to the presence of null alleles, and no linkage disequilibrium found. These microsatellite loci will be useful for assessing the genetic diversity and population structure of H cumingii.
KeywordHyriopsis Cumingii Microsatellite Polymorphism Genetic Diversity
Department[Yuan, Han-wen; Xu, Qiao-qing] Yangtze Univ, Engn Res Ctr Wetland Agr Middle Reaches Yangtze R, Minist Educ, Jinzhou 434025, Hubei Province, Peoples R China; [Guo, Long-gen] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
Funding OrganizationYangtze University, Jingzhou, Hubei Province, China[801200010112] ; Yangtze University, Jingzhou, Hubei Province, China[801200010112] ; Yangtze University, Jingzhou, Hubei Province, China[801200010112] ; Yangtze University, Jingzhou, Hubei Province, China[801200010112]
Indexed BySCI
Language英语
WOS IDWOS:000296392900008
Funding OrganizationYangtze University, Jingzhou, Hubei Province, China[801200010112] ; Yangtze University, Jingzhou, Hubei Province, China[801200010112] ; Yangtze University, Jingzhou, Hubei Province, China[801200010112] ; Yangtze University, Jingzhou, Hubei Province, China[801200010112]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16729
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXu, QQ (reprint author), Yangtze Univ, Engn Res Ctr Wetland Agr Middle Reaches Yangtze R, Minist Educ, Jinzhou 434025, Hubei Province, Peoples R China; xuqiaoqing@163.com
Recommended Citation
GB/T 7714
Yuan, Han-wen,Guo, Long-gen,Xu, Qiao-qing,et al. Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)[J]. IRANIAN JOURNAL OF FISHERIES SCIENCES,2011,10(4):631-640.
APA Yuan, Han-wen,Guo, Long-gen,Xu, Qiao-qing,Xu, QQ ,&xuqiaoqing@163.com.(2011).Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii).IRANIAN JOURNAL OF FISHERIES SCIENCES,10(4),631-640.
MLA Yuan, Han-wen,et al."Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)".IRANIAN JOURNAL OF FISHERIES SCIENCES 10.4(2011):631-640.
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