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题名: Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)
作者: Yuan, Han-wen ; Guo, Long-gen ; Xu, Qiao-qing
通讯作者: Xu, QQ (reprint author), Yangtze Univ, Engn Res Ctr Wetland Agr Middle Reaches Yangtze R, Minist Educ, Jinzhou 434025, Hubei Province, Peoples R China ; xuqiaoqing@163.com
关键词: Hyriopsis cumingii ; Microsatellite ; Polymorphism ; Genetic diversity
刊名: IRANIAN JOURNAL OF FISHERIES SCIENCES
发表日期: 2011
卷: 10, 期:4, 页:631-640
收录类别: SCI
部门归属: [Yuan, Han-wen; Xu, Qiao-qing] Yangtze Univ, Engn Res Ctr Wetland Agr Middle Reaches Yangtze R, Minist Educ, Jinzhou 434025, Hubei Province, Peoples R China; [Guo, Long-gen] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
资助者: Yangtze University, Jingzhou, Hubei Province, China[801200010112]
摘要: Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 primer pairs designed, 25 yielded scorable amplification products. Upon testing 30 individuals were sampled using the 25 pair primers from Dongting Lake of Hunan Province, China. Then thirteen polymorphic microsatellite markers were isolated and characterized. These loci exhibited high levels of genetic polymorphism, so the observed number of alleles per locus ranged from 4 to 9. The ranges of observed and expected heterozygosity were 0.2543 to 0.8913 and 0.3629 to 0.8217, respectively, and the average polymorphic information content was 0.5198. Two microsatellite loci were significantly deviated from Hardy-Weinberg equilibrium due to the presence of null alleles, and no linkage disequilibrium found. These microsatellite loci will be useful for assessing the genetic diversity and population structure of H cumingii.
语种: 英语
WOS记录号: WOS:000296392900008
ISSN号: 1562-2916
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16729
Appears in Collections:淡水生态学研究中心_期刊论文

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Yuan, Han-wen; Guo, Long-gen; Xu, Qiao-qing.Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii),IRANIAN JOURNAL OF FISHERIES SCIENCES,2011,10(4):631-640
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