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The role of cysteine conjugation in the detoxification of microcystin-LR in liver of bighead carp (Aristichthys nobilis): a field and laboratory study
Zhang, Dawen1,2; Yang, Qiang1; Xie, Ping1; Deng, Xuwei1; Chen, Jun1; Dai, Ming1; Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China
2012
Source PublicationECOTOXICOLOGY
ISSN0963-9292
Volume21Issue:1Pages:244-252
AbstractThe role of glutathione (GSH) and cysteine (Cys) conjugates in the detoxification of microcystin-LR (MC-LR) in bighead carp (Aristichthys nobilis) was examined under laboratory and field conditions. Wild individuals of bighead carp were collected from 5 eutrophic lakes along the Yangtze River, while in laboratory experiment, bighead carp were injected intraperitoneally with 500 mu g purified MC-LR/kg body weight (bw). Contents of MC-LR and its glutathione (MC-LR-GSH) and cysteine conjugates (MC-LR-Cys) in the liver of bighead carp were determined by liquid chromatography electrospray ionization mass spectrum (LC-ESI-MS). In laboratory experiment, low concentrations of MC-LR-GSH (mean: 0.042 mu g/g dry weight (DW)) were always detectable, and the mean ratio of MC-LR-Cys to MC-LR-GSH was 6.55. While, in field study, relatively high MC-LR-Cys concentration (mean: 0.22 mu g/g DW) was detected, whereas MC-LR-GSH was occasionally detectable, and the average ratio of MC-LR-Cys to MC-LR-GSH was as high as 71.49. A positive correlation was found between MC-LR-Cys concentration in the liver of bighead carp and MC-LR content in seston from the five lakes (r = 0.85). These results suggest that MC-LR-Cys might be much more important than MC-LR-GSH in the detoxification of MC-LR in fish liver, and that cysteine conjugation of MC-LR might be a physiological mechanism for the phytoplanktivorous bighead carp to counteract toxic cyanobacteria.; The role of glutathione (GSH) and cysteine (Cys) conjugates in the detoxification of microcystin-LR (MC-LR) in bighead carp (Aristichthys nobilis) was examined under laboratory and field conditions. Wild individuals of bighead carp were collected from 5 eutrophic lakes along the Yangtze River, while in laboratory experiment, bighead carp were injected intraperitoneally with 500 mu g purified MC-LR/kg body weight (bw). Contents of MC-LR and its glutathione (MC-LR-GSH) and cysteine conjugates (MC-LR-Cys) in the liver of bighead carp were determined by liquid chromatography electrospray ionization mass spectrum (LC-ESI-MS). In laboratory experiment, low concentrations of MC-LR-GSH (mean: 0.042 mu g/g dry weight (DW)) were always detectable, and the mean ratio of MC-LR-Cys to MC-LR-GSH was 6.55. While, in field study, relatively high MC-LR-Cys concentration (mean: 0.22 mu g/g DW) was detected, whereas MC-LR-GSH was occasionally detectable, and the average ratio of MC-LR-Cys to MC-LR-GSH was as high as 71.49. A positive correlation was found between MC-LR-Cys concentration in the liver of bighead carp and MC-LR content in seston from the five lakes (r = 0.85). These results suggest that MC-LR-Cys might be much more important than MC-LR-GSH in the detoxification of MC-LR in fish liver, and that cysteine conjugation of MC-LR might be a physiological mechanism for the phytoplanktivorous bighead carp to counteract toxic cyanobacteria.
SubtypeArticle
KeywordMicrocystins Mc-lr-cys Mc-lr-gsh Bighead Carp Detoxification
Department[Zhang, Dawen; Yang, Qiang; Xie, Ping; Deng, Xuwei; Chen, Jun; Dai, Ming] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China; [Zhang, Dawen] Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Key Lab Qual & Safety Agr Prod, Nanchang 330200, Jiangxi, Peoples R China
DOI10.1007/s10646-011-0783-1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMinistry of Science and Technology of China[2006FY110600]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; Ministry of Science and Technology of China[2006FY110600]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; Ministry of Science and Technology of China[2006FY110600]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; Ministry of Science and Technology of China[2006FY110600]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEcology ; Environmental Sciences ; Toxicology
WOS IDWOS:000298996900025
WOS KeywordHYPOPHTHALMICHTHYS-MOLITRIX ; HEPATOTOXIC MICROCYSTINS ; CYANOBACTERIAL TOXINS ; GLUTATHIONE CONJUGATE ; IN-VIVO ; IDENTIFICATION ; TOXICITY ; BRAZIL ; FISH ; LAKE
Funding OrganizationMinistry of Science and Technology of China[2006FY110600]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; Ministry of Science and Technology of China[2006FY110600]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; Ministry of Science and Technology of China[2006FY110600]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; Ministry of Science and Technology of China[2006FY110600]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16723
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorChen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
2.Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Key Lab Qual & Safety Agr Prod, Nanchang 330200, Jiangxi, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Dawen,Yang, Qiang,Xie, Ping,et al. The role of cysteine conjugation in the detoxification of microcystin-LR in liver of bighead carp (Aristichthys nobilis): a field and laboratory study[J]. ECOTOXICOLOGY,2012,21(1):244-252.
APA Zhang, Dawen.,Yang, Qiang.,Xie, Ping.,Deng, Xuwei.,Chen, Jun.,...&Chen, J .(2012).The role of cysteine conjugation in the detoxification of microcystin-LR in liver of bighead carp (Aristichthys nobilis): a field and laboratory study.ECOTOXICOLOGY,21(1),244-252.
MLA Zhang, Dawen,et al."The role of cysteine conjugation in the detoxification of microcystin-LR in liver of bighead carp (Aristichthys nobilis): a field and laboratory study".ECOTOXICOLOGY 21.1(2012):244-252.
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