| In vivo studies on the immunotoxic effects of microcystins on rabbit | |
| Yuan, Gailing1; Xie, Ping1,2; Zhang, Xuezhen1; Tang, Rong1; Gao, Yu1; Li, Dapeng1; Li, Li1; Xie, P (reprint author), Huazhong Agr Univ, Coll Fisheries, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr, Wuhan 430070, Peoples R China | |
| 2012-02-01 | |
| Source Publication | ENVIRONMENTAL TOXICOLOGY
 |
| ISSN | 1520-4081 |
| Volume | 27Issue:2Pages:83-89 |
| Abstract | Microcystins (MCs) are the toxic molecules produced by common cyanobacterium in freshwater blooms. Their toxicities raise severe health issues in livestock and human beings. In current study, the immunotoxic effects of MC-LR were investigated in rabbit through evaluating the dynamics of white blood cell (WBC) numbers and cytokine production such as interleukin-3 (IL-3), IL-4, IL-6, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha). MCs at the high dose (50 mu g MC-LReq kg-1) significantly induced increase in the WBC number but decrease in the Th1 (IFN-gamma, TNF-alpha) and Th2 (IL-3, IL-4, IL-6) production. In the low dose group(12.5 mu g MC-LReq kg-1), the number of WBC and the production of IFN-gamma, IFN-alpha, IL-4, IL-3, and IL-6 increased gradually in first 12 h, reach the peaks at 12 h, and dropped after 24 h. Significantly positive correlations were found between the cytokines production of IL-4 and IL-6, IFN-gamma and IFN-alpha, or IL-4 and IFN-gamma. In conclusion, MC-LR is able to disturb the rabbit immune system and there exists time-dose response relationship in the MC-LR-eliciting perturbation, which probably give a better insight into investigating the immunotoxicity mechanisms of MCs in vivo. (C) 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012.; Microcystins (MCs) are the toxic molecules produced by common cyanobacterium in freshwater blooms. Their toxicities raise severe health issues in livestock and human beings. In current study, the immunotoxic effects of MC-LR were investigated in rabbit through evaluating the dynamics of white blood cell (WBC) numbers and cytokine production such as interleukin-3 (IL-3), IL-4, IL-6, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha). MCs at the high dose (50 mu g MC-LReq kg-1) significantly induced increase in the WBC number but decrease in the Th1 (IFN-gamma, TNF-alpha) and Th2 (IL-3, IL-4, IL-6) production. In the low dose group(12.5 mu g MC-LReq kg-1), the number of WBC and the production of IFN-gamma, IFN-alpha, IL-4, IL-3, and IL-6 increased gradually in first 12 h, reach the peaks at 12 h, and dropped after 24 h. Significantly positive correlations were found between the cytokines production of IL-4 and IL-6, IFN-gamma and IFN-alpha, or IL-4 and IFN-gamma. In conclusion, MC-LR is able to disturb the rabbit immune system and there exists time-dose response relationship in the MC-LR-eliciting perturbation, which probably give a better insight into investigating the immunotoxicity mechanisms of MCs in vivo. (C) 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012. |
| Subtype | Article |
| Keyword | Microcystin-lr (Mc-lr) Immunotoxicity Rabbit Cytokine Wbc |
| Department | [Yuan, Gailing; Xie, Ping; Zhang, Xuezhen; Tang, Rong; Gao, Yu; Li, Dapeng; Li, Li] Huazhong Agr Univ, Coll Fisheries, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr, Wuhan 430070, Peoples R China; [Xie, Ping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
| DOI | 10.1002/tox.20615 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| Funding Organization | National Natural Science Foundation of China[30530170] ; National Natural Science Foundation of China[30530170] ; National Natural Science Foundation of China[30530170] ; National Natural Science Foundation of China[30530170] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Environmental Sciences & Ecology ; Toxicology ; Water Resources |
| WOS Subject | Environmental Sciences ; Toxicology ; Water Resources |
| WOS ID | WOS:000299038900003 |
| WOS Keyword | CYANOBACTERIAL TOXINS ; HEPATOTOXIC MICROCYSTINS ; IMMUNE FUNCTION ; MESSENGER-RNA ; NITRIC-OXIDE ; CYTOKINES ; LR ; MACROPHAGES ; AERUGINOSA ; EXPRESSION |
| Funding Organization | National Natural Science Foundation of China[30530170] ; National Natural Science Foundation of China[30530170] ; National Natural Science Foundation of China[30530170] ; National Natural Science Foundation of China[30530170] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/16719 |
| Collection | 淡水生态学研究中心_期刊论文 |
| Corresponding Author | Xie, P (reprint author), Huazhong Agr Univ, Coll Fisheries, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr, Wuhan 430070, Peoples R China |
| Affiliation | 1.Huazhong Agr Univ, Coll Fisheries, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Yuan, Gailing,Xie, Ping,Zhang, Xuezhen,et al. In vivo studies on the immunotoxic effects of microcystins on rabbit[J]. ENVIRONMENTAL TOXICOLOGY,2012,27(2):83-89. |
| APA | Yuan, Gailing.,Xie, Ping.,Zhang, Xuezhen.,Tang, Rong.,Gao, Yu.,...&Xie, P .(2012).In vivo studies on the immunotoxic effects of microcystins on rabbit.ENVIRONMENTAL TOXICOLOGY,27(2),83-89. |
| MLA | Yuan, Gailing,et al."In vivo studies on the immunotoxic effects of microcystins on rabbit".ENVIRONMENTAL TOXICOLOGY 27.2(2012):83-89. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| In vivo studies on t（445KB） | 开放获取 | -- | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment