| The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR | |
| Zhao, Sujuan1; Xie, Ping1; Li, Guangyu1,2; Jun, Chen1; Cai, Yan1; Xiong, Qian1; Zhao, Yanyan1; Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China | |
| 2012 | |
| Source Publication | PROTEOMICS
 |
| ISSN | 1615-9853 |
| Volume | 12Issue:2Pages:300-312 |
| Abstract | Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD50 (2000 mu g/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress.; Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD50 (2000 mu g/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress. |
| Subtype | Article |
| Keyword | 2-de Animal Proteomics Mc-rr Reproductive Toxicity Testis Ultrastructure |
| Department | [Zhao, Sujuan; Xie, Ping; Li, Guangyu; Jun, Chen; Cai, Yan; Xiong, Qian; Zhao, Yanyan] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Li, Guangyu] Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China |
| DOI | 10.1002/pmic.201100214 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology |
| WOS Subject | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| WOS ID | WOS:000299153500014 |
| WOS Keyword | CARP CYPRINUS-CARPIO ; ALDEHYDE DEHYDROGENASE ; INTERMEDIATE-FILAMENTS ; REPRODUCTIVE-SYSTEM ; OXIDATIVE STRESS ; HUMAN HEALTH ; LR ; LIVER ; GLUTATHIONE ; TOXICITY |
| Funding Organization | National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/16717 |
| Collection | 淡水生态学研究中心_期刊论文 |
| Corresponding Author | Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao, Sujuan,Xie, Ping,Li, Guangyu,et al. The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR[J]. PROTEOMICS,2012,12(2):300-312. |
| APA | Zhao, Sujuan.,Xie, Ping.,Li, Guangyu.,Jun, Chen.,Cai, Yan.,...&Xie, P .(2012).The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR.PROTEOMICS,12(2),300-312. |
| MLA | Zhao, Sujuan,et al."The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR".PROTEOMICS 12.2(2012):300-312. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| The proteomic study （527KB） | 开放获取 | -- | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment