IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR
Zhao, Sujuan1; Xie, Ping1; Li, Guangyu1,2; Jun, Chen1; Cai, Yan1; Xiong, Qian1; Zhao, Yanyan1; Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
2012
Source PublicationPROTEOMICS
ISSN1615-9853
Volume12Issue:2Pages:300-312
AbstractMicrocystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD50 (2000 mu g/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress.; Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD50 (2000 mu g/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress.
SubtypeArticle
Keyword2-de Animal Proteomics Mc-rr Reproductive Toxicity Testis Ultrastructure
Department[Zhao, Sujuan; Xie, Ping; Li, Guangyu; Jun, Chen; Cai, Yan; Xiong, Qian; Zhao, Yanyan] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Li, Guangyu] Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China
DOI10.1002/pmic.201100214
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS IDWOS:000299153500014
WOS KeywordCARP CYPRINUS-CARPIO ; ALDEHYDE DEHYDROGENASE ; INTERMEDIATE-FILAMENTS ; REPRODUCTIVE-SYSTEM ; OXIDATIVE STRESS ; HUMAN HEALTH ; LR ; LIVER ; GLUTATHIONE ; TOXICITY
Funding OrganizationNational Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16717
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Sujuan,Xie, Ping,Li, Guangyu,et al. The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR[J]. PROTEOMICS,2012,12(2):300-312.
APA Zhao, Sujuan.,Xie, Ping.,Li, Guangyu.,Jun, Chen.,Cai, Yan.,...&Xie, P .(2012).The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR.PROTEOMICS,12(2),300-312.
MLA Zhao, Sujuan,et al."The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR".PROTEOMICS 12.2(2012):300-312.
Files in This Item:
File Name/Size DocType Version Access License
The proteomic study (527KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao, Sujuan]'s Articles
[Xie, Ping]'s Articles
[Li, Guangyu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao, Sujuan]'s Articles
[Xie, Ping]'s Articles
[Li, Guangyu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao, Sujuan]'s Articles
[Xie, Ping]'s Articles
[Li, Guangyu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.