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题名: The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR
作者: Zhao, Sujuan1; Xie, Ping1; Li, Guangyu1, 2; Jun, Chen1; Cai, Yan1; Xiong, Qian1; Zhao, Yanyan1
通讯作者: Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
关键词: 2-DE ; Animal proteomics ; MC-RR ; Reproductive toxicity ; Testis ; Ultrastructure
刊名: PROTEOMICS
发表日期: 2012
DOI: 10.1002/pmic.201100214
卷: 12, 期:2, 页:300-312
收录类别: SCI
文章类型: Article
部门归属: [Zhao, Sujuan; Xie, Ping; Li, Guangyu; Jun, Chen; Cai, Yan; Xiong, Qian; Zhao, Yanyan] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Li, Guangyu] Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
摘要: Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD50 (2000 mu g/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress.
英文摘要: Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD50 (2000 mu g/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress.
关键词[WOS]: CARP CYPRINUS-CARPIO ; ALDEHYDE DEHYDROGENASE ; INTERMEDIATE-FILAMENTS ; REPRODUCTIVE-SYSTEM ; OXIDATIVE STRESS ; HUMAN HEALTH ; LR ; LIVER ; GLUTATHIONE ; TOXICITY
语种: 英语
WOS记录号: WOS:000299153500014
ISSN号: 1615-9853
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16717
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China

Recommended Citation:
Zhao, Sujuan; Xie, Ping; Li, Guangyu; Jun, Chen; Cai, Yan; Xiong, Qian; Zhao, Yanyan.The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR,PROTEOMICS,2012,12(2):300-312
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