IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
Genomic Profiling of MicroRNAs and Proteomics Reveals an Early Molecular Alteration Associated with Tumorigenesis Induced by MC-LR in Mice
Zhao, Yanyan1; Xie, Ping1; Fan, Huihui2; Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
2012-01-03
Source PublicationENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN0013-936X
Volume46Issue:1Pages:34-41
AbstractStudies have demonstrated that microcystins (MCs) can act as potential carcinogens and have caused serious risk to public environmental health. The molecular mechanisms of MC-induced susceptibility to carcinogenesis are largely unknown. In this study, we performed for the first time a comprehensive analysis of changes in microRNAs (miRNAs) and proteins expression in livers of mice treated with MC-LR. Utilizing microarray and two-dimensional gel electrophoresis (2-DE) analysis, we identified 37 miRNAs and 42 proteins significantly altered. Many aberrantly expressed miRNAs were related to various cancers (e.g., miR-125b, hepatocellular carcinoma; miR-21, leukemia; miR-16, chronic lymphocytic leukemia; miR-192, pituitary adenomas; miR-199a-3p, ovarian cancer; miR-34a, pancreatic cancer). Several miRNAs (e.g., miR-34a, miR-21) and proteins (e.g., TGM2, NDRG2) that play crucial roles in liver tumorigenesis were first found to be affected by MC-LR in mouse liver. MC-LR also altered the expression of a number of miRNAs and proteins involved in several pathways related to tumorigenesis, such as glutathione metabolism, VEGF signaling, and MAPK signaling pathway. Integration of post-transcriptomics, proteomics, and transcriptomics reveals that the networks miRNAs and their potential target genes and proteins involved in had a close association with carcinogenesis. These results provide an early molecular mechanism for liver tumorigenesis induced by MCs.; Studies have demonstrated that microcystins (MCs) can act as potential carcinogens and have caused serious risk to public environmental health. The molecular mechanisms of MC-induced susceptibility to carcinogenesis are largely unknown. In this study, we performed for the first time a comprehensive analysis of changes in microRNAs (miRNAs) and proteins expression in livers of mice treated with MC-LR. Utilizing microarray and two-dimensional gel electrophoresis (2-DE) analysis, we identified 37 miRNAs and 42 proteins significantly altered. Many aberrantly expressed miRNAs were related to various cancers (e.g., miR-125b, hepatocellular carcinoma; miR-21, leukemia; miR-16, chronic lymphocytic leukemia; miR-192, pituitary adenomas; miR-199a-3p, ovarian cancer; miR-34a, pancreatic cancer). Several miRNAs (e.g., miR-34a, miR-21) and proteins (e.g., TGM2, NDRG2) that play crucial roles in liver tumorigenesis were first found to be affected by MC-LR in mouse liver. MC-LR also altered the expression of a number of miRNAs and proteins involved in several pathways related to tumorigenesis, such as glutathione metabolism, VEGF signaling, and MAPK signaling pathway. Integration of post-transcriptomics, proteomics, and transcriptomics reveals that the networks miRNAs and their potential target genes and proteins involved in had a close association with carcinogenesis. These results provide an early molecular mechanism for liver tumorigenesis induced by MCs.
SubtypeArticle
Department[Zhao, Yanyan; Xie, Ping] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Fan, Huihui] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
DOI10.1021/es201514h
WOS HeadingsScience & Technology ; Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
Indexed BySCI
Language英语
WOS Research AreaEngineering ; Environmental Sciences & Ecology
WOS SubjectEngineering, Environmental ; Environmental Sciences
WOS IDWOS:000298762900006
WOS KeywordHUMAN HEPATOCELLULAR-CARCINOMA ; TUMOR-SUPPRESSOR GENE ; MICROCYSTIN-LR ; LIVER-CANCER ; EXPRESSION PROFILES ; RAT HEPATOCYTES ; MOUSE-LIVER ; IN-VIVO ; APOPTOSIS ; CELLS
Funding OrganizationNational Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
Citation statistics
Cited Times:44[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16715
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Yanyan,Xie, Ping,Fan, Huihui,et al. Genomic Profiling of MicroRNAs and Proteomics Reveals an Early Molecular Alteration Associated with Tumorigenesis Induced by MC-LR in Mice[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2012,46(1):34-41.
APA Zhao, Yanyan,Xie, Ping,Fan, Huihui,&Xie, P .(2012).Genomic Profiling of MicroRNAs and Proteomics Reveals an Early Molecular Alteration Associated with Tumorigenesis Induced by MC-LR in Mice.ENVIRONMENTAL SCIENCE & TECHNOLOGY,46(1),34-41.
MLA Zhao, Yanyan,et al."Genomic Profiling of MicroRNAs and Proteomics Reveals an Early Molecular Alteration Associated with Tumorigenesis Induced by MC-LR in Mice".ENVIRONMENTAL SCIENCE & TECHNOLOGY 46.1(2012):34-41.
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