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题名: Genomic Profiling of MicroRNAs and Proteomics Reveals an Early Molecular Alteration Associated with Tumorigenesis Induced by MC-LR in Mice
作者: Zhao, Yanyan1; Xie, Ping1; Fan, Huihui2
通讯作者: Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
刊名: ENVIRONMENTAL SCIENCE & TECHNOLOGY
发表日期: 2012-01-03
DOI: 10.1021/es201514h
卷: 46, 期:1, 页:34-41
收录类别: SCI
文章类型: Article
部门归属: [Zhao, Yanyan; Xie, Ping] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Fan, Huihui] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
WOS标题词: Science & Technology ; Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01]
类目[WOS]: Engineering, Environmental ; Environmental Sciences
研究领域[WOS]: Engineering ; Environmental Sciences & Ecology
摘要: Studies have demonstrated that microcystins (MCs) can act as potential carcinogens and have caused serious risk to public environmental health. The molecular mechanisms of MC-induced susceptibility to carcinogenesis are largely unknown. In this study, we performed for the first time a comprehensive analysis of changes in microRNAs (miRNAs) and proteins expression in livers of mice treated with MC-LR. Utilizing microarray and two-dimensional gel electrophoresis (2-DE) analysis, we identified 37 miRNAs and 42 proteins significantly altered. Many aberrantly expressed miRNAs were related to various cancers (e.g., miR-125b, hepatocellular carcinoma; miR-21, leukemia; miR-16, chronic lymphocytic leukemia; miR-192, pituitary adenomas; miR-199a-3p, ovarian cancer; miR-34a, pancreatic cancer). Several miRNAs (e.g., miR-34a, miR-21) and proteins (e.g., TGM2, NDRG2) that play crucial roles in liver tumorigenesis were first found to be affected by MC-LR in mouse liver. MC-LR also altered the expression of a number of miRNAs and proteins involved in several pathways related to tumorigenesis, such as glutathione metabolism, VEGF signaling, and MAPK signaling pathway. Integration of post-transcriptomics, proteomics, and transcriptomics reveals that the networks miRNAs and their potential target genes and proteins involved in had a close association with carcinogenesis. These results provide an early molecular mechanism for liver tumorigenesis induced by MCs.
英文摘要: Studies have demonstrated that microcystins (MCs) can act as potential carcinogens and have caused serious risk to public environmental health. The molecular mechanisms of MC-induced susceptibility to carcinogenesis are largely unknown. In this study, we performed for the first time a comprehensive analysis of changes in microRNAs (miRNAs) and proteins expression in livers of mice treated with MC-LR. Utilizing microarray and two-dimensional gel electrophoresis (2-DE) analysis, we identified 37 miRNAs and 42 proteins significantly altered. Many aberrantly expressed miRNAs were related to various cancers (e.g., miR-125b, hepatocellular carcinoma; miR-21, leukemia; miR-16, chronic lymphocytic leukemia; miR-192, pituitary adenomas; miR-199a-3p, ovarian cancer; miR-34a, pancreatic cancer). Several miRNAs (e.g., miR-34a, miR-21) and proteins (e.g., TGM2, NDRG2) that play crucial roles in liver tumorigenesis were first found to be affected by MC-LR in mouse liver. MC-LR also altered the expression of a number of miRNAs and proteins involved in several pathways related to tumorigenesis, such as glutathione metabolism, VEGF signaling, and MAPK signaling pathway. Integration of post-transcriptomics, proteomics, and transcriptomics reveals that the networks miRNAs and their potential target genes and proteins involved in had a close association with carcinogenesis. These results provide an early molecular mechanism for liver tumorigenesis induced by MCs.
关键词[WOS]: HUMAN HEPATOCELLULAR-CARCINOMA ; TUMOR-SUPPRESSOR GENE ; MICROCYSTIN-LR ; LIVER-CANCER ; EXPRESSION PROFILES ; RAT HEPATOCYTES ; MOUSE-LIVER ; IN-VIVO ; APOPTOSIS ; CELLS
语种: 英语
WOS记录号: WOS:000298762900006
ISSN号: 0013-936X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16715
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China

Recommended Citation:
Zhao, Yanyan; Xie, Ping; Fan, Huihui.Genomic Profiling of MicroRNAs and Proteomics Reveals an Early Molecular Alteration Associated with Tumorigenesis Induced by MC-LR in Mice,ENVIRONMENTAL SCIENCE & TECHNOLOGY,2012,46(1):34-41
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