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Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae
Li, Xuemei1,2; Yu, Yuhe1; Feng, Weisong1; Yan, Qingyun1; Gong, Yingchun1; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
2012-02-01
Source PublicationJOURNAL OF MICROBIOLOGY
ISSN1225-8873
Volume50Issue:1Pages:29-37
AbstractWe investigated the influence of host species on intestinal microbiota by comparing the gut bacterial community structure of four cohabitating freshwater fish larvae, silver carp, grass carp, bighead carp, and blunt snout bream, using denaturing gradient gel electrophoresis (DGGE) of the amplified 16S and 18S rRNA genes. Similarity clustering indicated that the intestinal microbiota derived from these four fish species could be divided into four groups based on 16S rRNA gene similarity, whereas the eukaryotic 18S rRNA genes showed no distinct groups. The water sample from the shared environment contained microbiota of an independent group as indicated by both 16S and 18S rRNA genes segments. The bacterial community structures were visualized using rank-abundance plots fitted with linear regression models. Results showed that the intestinal bacterial evenness was significantly different between species (P<0.05) and between species and the water sample (P<0.01). Thirty-five relatively dominant bands in DGGE patterns were sequenced and grouped into five major taxa: Proteobacteria (26), Actinobacteria (5), Bacteroidetes (1), Firmicutes (2), and Cyanobacterial (1). Six eukaryotes were detected by sequencing 18S rRNA genes segments. The present study suggests that the intestines of the four fish larvae, although reared in the same environment, contained distinct bacterial populations, while intestinal eukaryotic microorganisms were almost identical.; We investigated the influence of host species on intestinal microbiota by comparing the gut bacterial community structure of four cohabitating freshwater fish larvae, silver carp, grass carp, bighead carp, and blunt snout bream, using denaturing gradient gel electrophoresis (DGGE) of the amplified 16S and 18S rRNA genes. Similarity clustering indicated that the intestinal microbiota derived from these four fish species could be divided into four groups based on 16S rRNA gene similarity, whereas the eukaryotic 18S rRNA genes showed no distinct groups. The water sample from the shared environment contained microbiota of an independent group as indicated by both 16S and 18S rRNA genes segments. The bacterial community structures were visualized using rank-abundance plots fitted with linear regression models. Results showed that the intestinal bacterial evenness was significantly different between species (P<0.05) and between species and the water sample (P<0.01). Thirty-five relatively dominant bands in DGGE patterns were sequenced and grouped into five major taxa: Proteobacteria (26), Actinobacteria (5), Bacteroidetes (1), Firmicutes (2), and Cyanobacterial (1). Six eukaryotes were detected by sequencing 18S rRNA genes segments. The present study suggests that the intestines of the four fish larvae, although reared in the same environment, contained distinct bacterial populations, while intestinal eukaryotic microorganisms were almost identical.
SubtypeArticle
KeywordIntestinal Microbiota Denaturing Gradient Gel Electrophoresis Freshwater Fish Larvae Host Species Effects
Department[Li, Xuemei; Yu, Yuhe; Feng, Weisong; Yan, Qingyun; Gong, Yingchun] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China; [Li, Xuemei] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
DOI10.1007/s12275-012-1340-1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMajor State Basic Research Development Program of China[2009CB118705]; National Natural Science Foundation of China[31172084] ; Major State Basic Research Development Program of China[2009CB118705]; National Natural Science Foundation of China[31172084] ; Major State Basic Research Development Program of China[2009CB118705]; National Natural Science Foundation of China[31172084] ; Major State Basic Research Development Program of China[2009CB118705]; National Natural Science Foundation of China[31172084]
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000301013300004
WOS KeywordGRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; TROUT ONCORHYNCHUS-MYKISS ; EARLY-LIFE STAGES ; SALMO-SALAR L. ; BACTERIAL COMMUNITIES ; MOLECULAR CHARACTERIZATION ; PHYLOGENETIC ANALYSIS ; ZEBRAFISH GUT ; WATER FISH
Funding OrganizationMajor State Basic Research Development Program of China[2009CB118705]; National Natural Science Foundation of China[31172084] ; Major State Basic Research Development Program of China[2009CB118705]; National Natural Science Foundation of China[31172084] ; Major State Basic Research Development Program of China[2009CB118705]; National Natural Science Foundation of China[31172084] ; Major State Basic Research Development Program of China[2009CB118705]; National Natural Science Foundation of China[31172084]
Citation statistics
Cited Times:66[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16705
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorYu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Li, Xuemei,Yu, Yuhe,Feng, Weisong,et al. Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae[J]. JOURNAL OF MICROBIOLOGY,2012,50(1):29-37.
APA Li, Xuemei,Yu, Yuhe,Feng, Weisong,Yan, Qingyun,Gong, Yingchun,&Yu, YH .(2012).Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae.JOURNAL OF MICROBIOLOGY,50(1),29-37.
MLA Li, Xuemei,et al."Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae".JOURNAL OF MICROBIOLOGY 50.1(2012):29-37.
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