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题名: Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae
作者: Li, Xuemei1, 2; Yu, Yuhe1; Feng, Weisong1; Yan, Qingyun1; Gong, Yingchun1
通讯作者: Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
关键词: intestinal microbiota ; denaturing gradient gel electrophoresis ; freshwater fish larvae ; host species effects
刊名: JOURNAL OF MICROBIOLOGY
发表日期: 2012-02-01
DOI: 10.1007/s12275-012-1340-1
卷: 50, 期:1, 页:29-37
收录类别: SCI
文章类型: Article
部门归属: [Li, Xuemei; Yu, Yuhe; Feng, Weisong; Yan, Qingyun; Gong, Yingchun] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China; [Li, Xuemei] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Major State Basic Research Development Program of China[2009CB118705]; National Natural Science Foundation of China[31172084]
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
摘要: We investigated the influence of host species on intestinal microbiota by comparing the gut bacterial community structure of four cohabitating freshwater fish larvae, silver carp, grass carp, bighead carp, and blunt snout bream, using denaturing gradient gel electrophoresis (DGGE) of the amplified 16S and 18S rRNA genes. Similarity clustering indicated that the intestinal microbiota derived from these four fish species could be divided into four groups based on 16S rRNA gene similarity, whereas the eukaryotic 18S rRNA genes showed no distinct groups. The water sample from the shared environment contained microbiota of an independent group as indicated by both 16S and 18S rRNA genes segments. The bacterial community structures were visualized using rank-abundance plots fitted with linear regression models. Results showed that the intestinal bacterial evenness was significantly different between species (P<0.05) and between species and the water sample (P<0.01). Thirty-five relatively dominant bands in DGGE patterns were sequenced and grouped into five major taxa: Proteobacteria (26), Actinobacteria (5), Bacteroidetes (1), Firmicutes (2), and Cyanobacterial (1). Six eukaryotes were detected by sequencing 18S rRNA genes segments. The present study suggests that the intestines of the four fish larvae, although reared in the same environment, contained distinct bacterial populations, while intestinal eukaryotic microorganisms were almost identical.
英文摘要: We investigated the influence of host species on intestinal microbiota by comparing the gut bacterial community structure of four cohabitating freshwater fish larvae, silver carp, grass carp, bighead carp, and blunt snout bream, using denaturing gradient gel electrophoresis (DGGE) of the amplified 16S and 18S rRNA genes. Similarity clustering indicated that the intestinal microbiota derived from these four fish species could be divided into four groups based on 16S rRNA gene similarity, whereas the eukaryotic 18S rRNA genes showed no distinct groups. The water sample from the shared environment contained microbiota of an independent group as indicated by both 16S and 18S rRNA genes segments. The bacterial community structures were visualized using rank-abundance plots fitted with linear regression models. Results showed that the intestinal bacterial evenness was significantly different between species (P<0.05) and between species and the water sample (P<0.01). Thirty-five relatively dominant bands in DGGE patterns were sequenced and grouped into five major taxa: Proteobacteria (26), Actinobacteria (5), Bacteroidetes (1), Firmicutes (2), and Cyanobacterial (1). Six eukaryotes were detected by sequencing 18S rRNA genes segments. The present study suggests that the intestines of the four fish larvae, although reared in the same environment, contained distinct bacterial populations, while intestinal eukaryotic microorganisms were almost identical.
关键词[WOS]: GRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; TROUT ONCORHYNCHUS-MYKISS ; EARLY-LIFE STAGES ; SALMO-SALAR L. ; BACTERIAL COMMUNITIES ; MOLECULAR CHARACTERIZATION ; PHYLOGENETIC ANALYSIS ; ZEBRAFISH GUT ; WATER FISH
语种: 英语
WOS记录号: WOS:000301013300004
ISSN号: 1225-8873
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16705
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Li, Xuemei; Yu, Yuhe; Feng, Weisong; Yan, Qingyun; Gong, Yingchun.Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae,JOURNAL OF MICROBIOLOGY,2012,50(1):29-37
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