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Molecular cloning and characterization of interferon regulatory factor 1 (IRF-1), IRF-2 and IRF-5 in the chondrostean paddlefish Polyodon spathula and their phylogenetic importance in the Osteichthyes
Gan Xiaoni1,2,3; Chen Zhuo2; Wang Xuzhen2; Wang Dengqiang2; Chen Xinwen1; Chen, XW (reprint author), Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei Province, Peoples R China
2012
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume36Issue:1Pages:74-84
AbstractThe interferon regulatory factor (IRF) with its 10 members is a very important gene family related to innate immunity. Currently, most fish IRFs reported are from bony fish (teleosts). Cloning and sequencing of IRFs from chondrosteans, the so-called "ancient fish" including sturgeon, paddlefish, bichir and gar, are absent from the literature. In this study, three IRF genes PsIRF-1, PsIRF-2 and PsIRF-5, were cloned and characterized from the paddlefish (Polyodon spathula). PsIRF-1 includes an open reading frame (ORF) of 972 bp that encodes a putative protein of 324 amino acids; PsIRF-2 includes an ORF of 1023 bp encoding 341 amino acids and P5IRF-5 includes an ORF of 1491 bp that encodes 497 amino acids. The P5IRF-5 gene structure is similar to those in mammals but differs from those in teleosts in the first and second exons. Phylogenetic studies of the putative amino acid sequences of PsIRF-1, PsIRF-2 and PsIRF-5 based on the neighbor-joining and Bayesian inference method for Osteichthyes found widely accepted inter-relationships among actinopterygians and tetrapods. Reverse Transcription Polymerase Chain Reaction (RT-PCR) analysis of PsIRF-1, PsIRF-2 and P5IRF-5 in different paddlefish tissues shows higher levels of expression in gill, spleen and head kidney. Poly (I: C) (polyinosinic-polycytidylic acid) stimulation in vivo up-regulated PsIRF-1 and PsIRF-2 expression, while P5IRF-5 gene expression did not respond to the challenge of Poly (I: C). (C) 2011 Elsevier Ltd. All rights reserved.; The interferon regulatory factor (IRF) with its 10 members is a very important gene family related to innate immunity. Currently, most fish IRFs reported are from bony fish (teleosts). Cloning and sequencing of IRFs from chondrosteans, the so-called "ancient fish" including sturgeon, paddlefish, bichir and gar, are absent from the literature. In this study, three IRF genes PsIRF-1, PsIRF-2 and PsIRF-5, were cloned and characterized from the paddlefish (Polyodon spathula). PsIRF-1 includes an open reading frame (ORF) of 972 bp that encodes a putative protein of 324 amino acids; PsIRF-2 includes an ORF of 1023 bp encoding 341 amino acids and P5IRF-5 includes an ORF of 1491 bp that encodes 497 amino acids. The P5IRF-5 gene structure is similar to those in mammals but differs from those in teleosts in the first and second exons. Phylogenetic studies of the putative amino acid sequences of PsIRF-1, PsIRF-2 and PsIRF-5 based on the neighbor-joining and Bayesian inference method for Osteichthyes found widely accepted inter-relationships among actinopterygians and tetrapods. Reverse Transcription Polymerase Chain Reaction (RT-PCR) analysis of PsIRF-1, PsIRF-2 and P5IRF-5 in different paddlefish tissues shows higher levels of expression in gill, spleen and head kidney. Poly (I: C) (polyinosinic-polycytidylic acid) stimulation in vivo up-regulated PsIRF-1 and PsIRF-2 expression, while P5IRF-5 gene expression did not respond to the challenge of Poly (I: C). (C) 2011 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordIfn Innate Immunity Psirf-1 Psirf-2 Psirf-5 Polyodon Spathula Phylogeny
Department[Gan Xiaoni; Chen Xinwen] Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei Province, Peoples R China; [Gan Xiaoni; Chen Zhuo; Wang Xuzhen; Wang Dengqiang] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Gan Xiaoni] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
DOI10.1016/j.dci.2011.06.003
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNatural Science Foundation of China[HCV2009CB522500, 30950006] ; Natural Science Foundation of China[HCV2009CB522500, 30950006] ; Natural Science Foundation of China[HCV2009CB522500, 30950006] ; Natural Science Foundation of China[HCV2009CB522500, 30950006]
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000299390300008
WOS KeywordGENE-EXPRESSION ; TRANSCRIPTION ; ACTIVATION ; PROMOTER ; GENERATION ; INDUCTION ; SEQUENCE ; CELLS ; FISH ; RNA
Funding OrganizationNatural Science Foundation of China[HCV2009CB522500, 30950006] ; Natural Science Foundation of China[HCV2009CB522500, 30950006] ; Natural Science Foundation of China[HCV2009CB522500, 30950006] ; Natural Science Foundation of China[HCV2009CB522500, 30950006]
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16703
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorChen, XW (reprint author), Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei Province, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei Province, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Gan Xiaoni,Chen Zhuo,Wang Xuzhen,et al. Molecular cloning and characterization of interferon regulatory factor 1 (IRF-1), IRF-2 and IRF-5 in the chondrostean paddlefish Polyodon spathula and their phylogenetic importance in the Osteichthyes[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,36(1):74-84.
APA Gan Xiaoni,Chen Zhuo,Wang Xuzhen,Wang Dengqiang,Chen Xinwen,&Chen, XW .(2012).Molecular cloning and characterization of interferon regulatory factor 1 (IRF-1), IRF-2 and IRF-5 in the chondrostean paddlefish Polyodon spathula and their phylogenetic importance in the Osteichthyes.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,36(1),74-84.
MLA Gan Xiaoni,et al."Molecular cloning and characterization of interferon regulatory factor 1 (IRF-1), IRF-2 and IRF-5 in the chondrostean paddlefish Polyodon spathula and their phylogenetic importance in the Osteichthyes".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 36.1(2012):74-84.
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