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题名: Preparation of protoplasts from Chlorella protothecoides
作者: Lu, Yongzhong1; Kong, Renqiu2; Hu, Liwei1
通讯作者: Lu, Y.(luyz@yahoo.com)
关键词: Protoplast ; Cell wall ; Enzymatic digestion ; Chlorella ; Regeneration
刊名: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
发表日期: 2012-04-01
DOI: 10.1007/s11274-011-0963-4
卷: 28, 期:4, 页:1827-1830
收录类别: SCI
文章类型: Article
部门归属: (1) Biology Department, Qingdao University of Science and Technology, Qingdao, 266042, China; (2) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
摘要: This paper describes an enzymatic method for yielding protoplasts from the microalga Chlorella protothecoides. Four kinds of commercially available enzymes were tested. The enzymatic digestion was optimal with 2% cellulase R-10 and 1% snailase prepared in 25 mM Tris buffer (pH 6.0) containing 0.6 M D-mannitol, and the protoplast density could reach the peak after treatment at 30°C for 16 h. Nearly all liberated protoplasts were green in the presence of 0.01% phenosafranin, indicating their high viability. The regeneration rate was about 70% when 0.6 M D-mannitol was used as an osmotic stabilizer in the regeneration medium. This protocol will find useful applications in genetic studies of this algal species.
英文摘要: This paper describes an enzymatic method for yielding protoplasts from the microalga Chlorella protothecoides. Four kinds of commercially available enzymes were tested. The enzymatic digestion was optimal with 2% cellulase R-10 and 1% snailase prepared in 25 mM Tris buffer (pH 6.0) containing 0.6 M D-mannitol, and the protoplast density could reach the peak after treatment at 30A degrees C for 16 h. Nearly all liberated protoplasts were green in the presence of 0.01% phenosafranin, indicating their high viability. The regeneration rate was about 70% when 0.6 M D-mannitol was used as an osmotic stabilizer in the regeneration medium. This protocol will find useful applications in genetic studies of this algal species.
关键词[WOS]: CELL-WALL ; INDUCTION ; ALGAE
语种: 英语
WOS记录号: WOS:000302292500054
ISSN号: 0959-3993
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16686
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Qingdao Univ Sci & Technol, Dept Biol, Qingdao 266042, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China

Recommended Citation:
Lu, Yongzhong (1); Kong, Renqiu (2); Hu, Liwei (1) .Preparation of protoplasts from Chlorella protothecoides,World Journal of Microbiology and Biotechnology,2011,():40547
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文件名: Preparation of protoplasts from Chlorella protothecoides.pdf
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