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Genes Associated with 2-Methylisoborneol Biosynthesis in Cyanobacteria: Isolation, Characterization, and Expression in Response to Light
Wang, Zhongjie; Xu, Yao; Shao, Jihai; Wang, Jie; Li, Renhui; Wang, ZJ (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Peoples R China; reli@ihb.ac.cn
2011
Source PublicationPLOS ONE
ISSN1932-6203
Volume6Issue:4Pages:e18665
AbstractThe volatile microbial metabolite 2-methylisoborneol (2-MIB) is a root cause of taste and odor issues in freshwater. Although current evidence suggests that 2-MIB is not toxic, this compound degrades water quality and presents problems for water treatment. To address these issues, cyanobacteria and actinomycetes, the major producers of 2-MIB, have been investigated extensively. In this study, two 2-MIB producing strains, coded as Pseudanabaena sp. and Planktothricoids raciborskii, were used in order to elucidate the genetic background, light regulation, and biochemical mechanisms of 2-MIB biosynthesis in cyanobacteria. Genome walking and PCR methods revealed that two adjacent genes, SAM-dependent methyltransferanse gene and monoterpene cyclase gene, are responsible for GPP methylation and subsequent cyclization to 2-MIB in cyanobacteria. These two genes are located in between two homologous cyclic nucleotide-binding protein genes that may be members of the Crp-Fnr regulator family. Together, this sequence of genes forms a putative operon. The synthesis of 2-MIB is similar in cyanobacteria and actinomycetes. Comparison of the gene arrangement and functional sites between cyanobacteria and other organisms revealed that gene recombination and gene transfer probably occurred during the evolution of 2-MIB-associated genes. All the microorganisms examined have a common origin of 2-MIB biosynthesis capacity, but cyanobacteria represent a unique evolutionary lineage. Gene expression analysis suggested that light is a crucial, but not the only, active regulatory factor for the transcription of 2-MIB synthesis genes. This light-regulated process is immediate and transient. This study is the first to identify the genetic background and evolution of 2-MIB biosynthesis in cyanobacteria, thus enhancing current knowledge on 2-MIB contamination of freshwater.
KeywordGeosmin Production Odorous Compounds United-states Identification Taste Growth Waters Lake
Department[Wang, Zhongjie; Li, Renhui] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Peoples R China; [Wang, Zhongjie] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China; [Xu, Yao] Nanjing Normal Univ, Coll Geog Sci, Nanjing, Peoples R China; [Shao, Jihai] Hunan Agr Univ, Resources & Environm Coll, Changsha, Hunan, Peoples R China; [Wang, Jie] Shanxi Univ, Coll Life Sci, Taiyuan, Peoples R China
Subject AreaLife Sciences & Biomedicine - Other Topics
Funding OrganizationState Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2008ZX07105-006, 2009ZX07101-013]; Chinese Academy of Science[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2008ZX07105-006, 2009ZX07101-013]; Chinese Academy of Science[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2008ZX07105-006, 2009ZX07101-013]; Chinese Academy of Science[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2008ZX07105-006, 2009ZX07101-013]; Chinese Academy of Science[082303-1-501]
Indexed BySCI
Language英语
WOS IDWOS:000289238700035
Funding OrganizationState Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2008ZX07105-006, 2009ZX07101-013]; Chinese Academy of Science[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2008ZX07105-006, 2009ZX07101-013]; Chinese Academy of Science[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2008ZX07105-006, 2009ZX07101-013]; Chinese Academy of Science[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2008ZX07105-006, 2009ZX07101-013]; Chinese Academy of Science[082303-1-501]
Citation statistics
Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16479
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorWang, ZJ (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Peoples R China; reli@ihb.ac.cn
Recommended Citation
GB/T 7714
Wang, Zhongjie,Xu, Yao,Shao, Jihai,et al. Genes Associated with 2-Methylisoborneol Biosynthesis in Cyanobacteria: Isolation, Characterization, and Expression in Response to Light[J]. PLOS ONE,2011,6(4):e18665.
APA Wang, Zhongjie.,Xu, Yao.,Shao, Jihai.,Wang, Jie.,Li, Renhui.,...&reli@ihb.ac.cn.(2011).Genes Associated with 2-Methylisoborneol Biosynthesis in Cyanobacteria: Isolation, Characterization, and Expression in Response to Light.PLOS ONE,6(4),e18665.
MLA Wang, Zhongjie,et al."Genes Associated with 2-Methylisoborneol Biosynthesis in Cyanobacteria: Isolation, Characterization, and Expression in Response to Light".PLOS ONE 6.4(2011):e18665.
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