中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
学科主题: Microbiology
题名: Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris
作者: Wang, Yali1; Liu, Xiaoxiang1; Gao, Hong1; Xu, Xudong1
通讯作者: Xu, XD (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China ; xux@ihb.ac.cn
关键词: Chlorella vulgaris ; Antarctic strain ; temperate strain ; hiC6 ; tandem array
刊名: FEMS MICROBIOLOGY LETTERS
发表日期: 2011-12-01
DOI: 10.1111/j.1574-6968.2011.02420.x
卷: 325, 期:2, 页:130-139
收录类别: SCI
文章类型: Article
部门归属: [Wang, Yali; Liu, Xiaoxiang; Gao, Hong; Xu, Xudong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332]
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
摘要: HIC6 is a group-3 late embryogenesis abundant protein found in Chlorella vulgaris. In the Antarctic strain NJ-7 of this unicellular green alga, it is encoded by a tandem array of five hiC6 genes (designated as NJ7hiC6-1, -2, -3, -4 and -5); in the temperate strain UTEX259, it is encoded by four hiC6 genes in tandem (designated as 259hiC6-1, -2, -3 and -4). Except for NJ7hiC6-3 and -4, the encoding regions of all other hiC6 genes differ from each other by 219 bp in each strain. Based on RT-PCR and sequencing of total hiC6 cDNA clones, the relative transcript abundance of each hiC6 gene was evaluated. NJ7hiC6-2 and 259hiC6-2 were not expressed or expressed at low levels, whereas 259hiC6-1 and NJ7hiC6-3/4 exhibited the highest hiC6 transcript levels in the respective strains. In vitro assays showed that different isoforms of HIC6 provided almost identical cryoprotection of lactate dehydrogenase. Our studies suggest that the formation of the tandem arrays of hiC6 in Chlorella is a process of gene duplications accompanied by gene expression divergence.
英文摘要: HIC6 is a group-3 late embryogenesis abundant protein found in Chlorella vulgaris. In the Antarctic strain NJ-7 of this unicellular green alga, it is encoded by a tandem array of five hiC6 genes (designated as NJ7hiC6-1, -2, -3, -4 and -5); in the temperate strain UTEX259, it is encoded by four hiC6 genes in tandem (designated as 259hiC6-1, -2, -3 and -4). Except for NJ7hiC6-3 and -4, the encoding regions of all other hiC6 genes differ from each other by 219 bp in each strain. Based on RT-PCR and sequencing of total hiC6 cDNA clones, the relative transcript abundance of each hiC6 gene was evaluated. NJ7hiC6-2 and 259hiC6-2 were not expressed or expressed at low levels, whereas 259hiC6-1 and NJ7hiC6-3/4 exhibited the highest hiC6 transcript levels in the respective strains. In vitro assays showed that different isoforms of HIC6 provided almost identical cryoprotection of lactate dehydrogenase. Our studies suggest that the formation of the tandem arrays of hiC6 in Chlorella is a process of gene duplications accompanied by gene expression divergence.
关键词[WOS]: EMBRYOGENESIS ABUNDANT PROTEINS ; HARDENING-INDUCED GENES ; FREEZING TOLERANCE ; LEA PROTEINS ; IDENTIFICATION ; EXPRESSION ; C-27 ; ELLIPSOIDEA ; DEHYDRINS ; ENCODES
语种: 英语
WOS记录号: WOS:000297208600005
ISSN号: 0378-1097
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16475
Appears in Collections:藻类生物学及应用研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris.pdf(646KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China

Recommended Citation:
Wang, Yali; Liu, Xiaoxiang; Gao, Hong; Xu, Xudong.Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris,FEMS MICROBIOLOGY LETTERS,2011,325(2):130-139
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Yali]'s Articles
[Liu, Xiaoxiang]'s Articles
[Gao, Hong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Yali]‘s Articles
[Liu, Xiaoxiang]‘s Articles
[Gao, Hong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace