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Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris
Wang, Yali; Liu, Xiaoxiang; Gao, Hong; Xu, Xudong; Xu, XD (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China; xux@ihb.ac.cn
2011
Source PublicationFEMS MICROBIOLOGY LETTERS
ISSN0378-1097
Volume325Issue:2Pages:130-139
AbstractHIC6 is a group-3 late embryogenesis abundant protein found in Chlorella vulgaris. In the Antarctic strain NJ-7 of this unicellular green alga, it is encoded by a tandem array of five hiC6 genes (designated as NJ7hiC6-1, -2, -3, -4 and -5); in the temperate strain UTEX259, it is encoded by four hiC6 genes in tandem (designated as 259hiC6-1, -2, -3 and -4). Except for NJ7hiC6-3 and -4, the encoding regions of all other hiC6 genes differ from each other by 219 bp in each strain. Based on RT-PCR and sequencing of total hiC6 cDNA clones, the relative transcript abundance of each hiC6 gene was evaluated. NJ7hiC6-2 and 259hiC6-2 were not expressed or expressed at low levels, whereas 259hiC6-1 and NJ7hiC6-3/4 exhibited the highest hiC6 transcript levels in the respective strains. In vitro assays showed that different isoforms of HIC6 provided almost identical cryoprotection of lactate dehydrogenase. Our studies suggest that the formation of the tandem arrays of hiC6 in Chlorella is a process of gene duplications accompanied by gene expression divergence.
KeywordChlorella Vulgaris Antarctic Strain Temperate Strain Hic6 Tandem Array
Department[Wang, Yali; Liu, Xiaoxiang; Gao, Hong; Xu, Xudong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China
Subject AreaMicrobiology
Funding OrganizationChinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332]
Indexed BySCI
Language英语
WOS IDWOS:000297208600005
Funding OrganizationChinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16475
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, XD (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China; xux@ihb.ac.cn
Recommended Citation
GB/T 7714
Wang, Yali,Liu, Xiaoxiang,Gao, Hong,et al. Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris[J]. FEMS MICROBIOLOGY LETTERS,2011,325(2):130-139.
APA Wang, Yali,Liu, Xiaoxiang,Gao, Hong,Xu, Xudong,Xu, XD ,&xux@ihb.ac.cn.(2011).Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris.FEMS MICROBIOLOGY LETTERS,325(2),130-139.
MLA Wang, Yali,et al."Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris".FEMS MICROBIOLOGY LETTERS 325.2(2011):130-139.
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