| Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis | |
| Xie, Xun-wei; Liu, Jing-Xia; Hu, Bo; Xiao, Wuhan; Xie, XW (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China; w-xiao@ihb.ac.cn | |
| 2011 | |
| Source Publication | PLOS ONE
 |
| ISSN | 1932-6203 |
| Volume | 6Issue:9Pages:e24469 |
| Abstract | FOXO genes are involved in many aspects of development and vascular homeostasis by regulating cell apoptosis, proliferation, and the control of oxidative stress. In addition, FOXO genes have been showed to inhibit Wnt/beta-catenin signaling by competing with T cell factor to bind to beta-catenin. However, how important of this inhibition in vivo, particularly in embryogenesis is still unknown. To demonstrate the roles of FOXO genes in embryogenesis will help us to further understand their relevant physiological functions. Zebrafish foxo3b gene, an orthologue of mammalian FOXO3, was expressed maternally and distributed ubiquitously during early embryogenesis and later restricted to brain. After morpholino-mediated knockdown of foxo3b, the zebrafish embryos exhibited defects in axis and neuroectoderm formation, suggesting its critical role in early embryogenesis. The embryo-developmental marker gene staining at different stages, phenotype analysis and rescue assays revealed that foxo3b acted its role through negatively regulating both maternal and zygotic Wnt/beta-catenin signaling. Moreover, we found that foxo3b could interact with zebrafish beta-catenin1 and beta-catenin2 to suppress their transactivation in vitro and in vivo, further confirming its role relevant to the inhibition of Wnt/beta-catenin signaling. Taken together, we revealed that foxo3b played a very important role in embryogenesis and negatively regulated maternal and zygotic Wnt/beta-catenin signaling by directly interacting with both beta-catenin1 and beta-catenin2. Our studies provide an in vivo model for illustrating function of FOXO transcription factors in embryogenesis. |
| Keyword | Forkhead Transcription Factor Posterior Mesoderm Formation Beta-catenin Homeobox Gene Organizer Formation Xenopus Mesoderm Dorsal Organizer Expression Gastrulation Proteins |
| Department | [Xie, Xun-wei; Liu, Jing-Xia; Hu, Bo; Xiao, Wuhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China |
| Subject Area | Life Sciences & Biomedicine - Other Topics |
| Funding Organization | 973 grant[2010CB126306, 2007CB815705]; NSFC[91019008, 31071212, 30971667, 20890113]; National Transgene Project[2009ZX08010-021B]; Chinese Academy of Science[KSCX2-Y-W-N-020, KSCX2-EW-Q-12] ; 973 grant[2010CB126306, 2007CB815705]; NSFC[91019008, 31071212, 30971667, 20890113]; National Transgene Project[2009ZX08010-021B]; Chinese Academy of Science[KSCX2-Y-W-N-020, KSCX2-EW-Q-12] ; 973 grant[2010CB126306, 2007CB815705]; NSFC[91019008, 31071212, 30971667, 20890113]; National Transgene Project[2009ZX08010-021B]; Chinese Academy of Science[KSCX2-Y-W-N-020, KSCX2-EW-Q-12] ; 973 grant[2010CB126306, 2007CB815705]; NSFC[91019008, 31071212, 30971667, 20890113]; National Transgene Project[2009ZX08010-021B]; Chinese Academy of Science[KSCX2-Y-W-N-020, KSCX2-EW-Q-12] |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000294802500050 |
| Funding Organization | 973 grant[2010CB126306, 2007CB815705]; NSFC[91019008, 31071212, 30971667, 20890113]; National Transgene Project[2009ZX08010-021B]; Chinese Academy of Science[KSCX2-Y-W-N-020, KSCX2-EW-Q-12] ; 973 grant[2010CB126306, 2007CB815705]; NSFC[91019008, 31071212, 30971667, 20890113]; National Transgene Project[2009ZX08010-021B]; Chinese Academy of Science[KSCX2-Y-W-N-020, KSCX2-EW-Q-12] ; 973 grant[2010CB126306, 2007CB815705]; NSFC[91019008, 31071212, 30971667, 20890113]; National Transgene Project[2009ZX08010-021B]; Chinese Academy of Science[KSCX2-Y-W-N-020, KSCX2-EW-Q-12] ; 973 grant[2010CB126306, 2007CB815705]; NSFC[91019008, 31071212, 30971667, 20890113]; National Transgene Project[2009ZX08010-021B]; Chinese Academy of Science[KSCX2-Y-W-N-020, KSCX2-EW-Q-12] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/16471 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Xie, XW (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China; w-xiao@ihb.ac.cn |
| Recommended Citation GB/T 7714 | Xie, Xun-wei,Liu, Jing-Xia,Hu, Bo,et al. Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis[J]. PLOS ONE,2011,6(9):e24469. |
| APA | Xie, Xun-wei,Liu, Jing-Xia,Hu, Bo,Xiao, Wuhan,Xie, XW ,&w-xiao@ihb.ac.cn.(2011).Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis.PLOS ONE,6(9),e24469. |
| MLA | Xie, Xun-wei,et al."Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis".PLOS ONE 6.9(2011):e24469. |
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