IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Identification and Characterization of Alternative Promoters, Transcripts and Protein Isoforms of Zebrafish R2 Gene
Shang, Hanqiao; Li, Qing; Feng, Guohui; Cui, Zongbin; Shang, HQ (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China; zbcui@ihb.ac.cn
2011
Source PublicationPLOS ONE
ISSN1932-6203
Volume6Issue:8Pages:e24089
AbstractRibonucleotide reductase (RNR) is the rate-limiting enzyme in the de novo synthesis of deoxyribonucleoside triphosphates. Expression of RNR subunits is closely associated with DNA replication and repair. Mammalian RNR M2 subunit (R2) functions exclusively in DNA replication of normal cells due to its S phase-specific expression and late mitotic degradation. Herein, we demonstrate the control of R2 expression through alternative promoters, splicing and polyadenylation sites in zebrafish. Three functional R2 promoters were identified to generate six transcript variants with distinct 5' termini. The proximal promoter contains a conserved E2F binding site and two CCAAT boxes, which are crucial for the transcription of R2 gene during cell cycle. Activity of the distal promoter can be induced by DNA damage to generate four transcript variants through alternative splicing. In addition, two novel splice variants were found to encode distinct N-truncated R2 isoforms containing residues for enzymatic activity but no KEN box essential for its proteolysis. These two N-truncated R2 isoforms remained in the cytoplasm and were able to interact with RNR M1 subunit (R1). Thus, our results suggest that multilayered mechanisms control the differential expression and function of zebrafish R2 gene during cell cycle and under genotoxic stress.
KeywordRibonucleotide Reductase R2 Cell-cycle Regulation Dna-damaging Agents Small-subunit Messenger-rna S-phase Saccharomyces-cerevisiae Mammalian-cells Fission Yeast Expression
Department[Shang, Hanqiao; Li, Qing; Feng, Guohui; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China; [Shang, Hanqiao; Feng, Guohui] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Subject AreaLife Sciences & Biomedicine - Other Topics
Funding OrganizationNational Natural Science Foundation of China[30671602, 30871442]; National Basic Research Program of China[2012CB944500]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[30671602, 30871442]; National Basic Research Program of China[2012CB944500]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[30671602, 30871442]; National Basic Research Program of China[2012CB944500]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[30671602, 30871442]; National Basic Research Program of China[2012CB944500]; National High-tech RD (863) Program[2007AA10Z164]
Indexed BySCI
Language英语
WOS IDWOS:000295832000093
Funding OrganizationNational Natural Science Foundation of China[30671602, 30871442]; National Basic Research Program of China[2012CB944500]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[30671602, 30871442]; National Basic Research Program of China[2012CB944500]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[30671602, 30871442]; National Basic Research Program of China[2012CB944500]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[30671602, 30871442]; National Basic Research Program of China[2012CB944500]; National High-tech RD (863) Program[2007AA10Z164]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16467
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorShang, HQ (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China; zbcui@ihb.ac.cn
Recommended Citation
GB/T 7714
Shang, Hanqiao,Li, Qing,Feng, Guohui,et al. Identification and Characterization of Alternative Promoters, Transcripts and Protein Isoforms of Zebrafish R2 Gene[J]. PLOS ONE,2011,6(8):e24089.
APA Shang, Hanqiao,Li, Qing,Feng, Guohui,Cui, Zongbin,Shang, HQ ,&zbcui@ihb.ac.cn.(2011).Identification and Characterization of Alternative Promoters, Transcripts and Protein Isoforms of Zebrafish R2 Gene.PLOS ONE,6(8),e24089.
MLA Shang, Hanqiao,et al."Identification and Characterization of Alternative Promoters, Transcripts and Protein Isoforms of Zebrafish R2 Gene".PLOS ONE 6.8(2011):e24089.
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