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学科主题: Life Sciences & Biomedicine - Other Topics
题名: Identification and Characterization of Alternative Promoters, Transcripts and Protein Isoforms of Zebrafish R2 Gene
作者: Shang, Hanqiao ; Li, Qing ; Feng, Guohui ; Cui, Zongbin
通讯作者: Shang, HQ (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China ; zbcui@ihb.ac.cn
关键词: RIBONUCLEOTIDE REDUCTASE R2 ; CELL-CYCLE REGULATION ; DNA-DAMAGING AGENTS ; SMALL-SUBUNIT ; MESSENGER-RNA ; S-PHASE ; SACCHAROMYCES-CEREVISIAE ; MAMMALIAN-CELLS ; FISSION YEAST ; EXPRESSION
刊名: PLOS ONE
发表日期: 2011
卷: 6, 期:8, 页:e24089
收录类别: SCI
部门归属: [Shang, Hanqiao; Li, Qing; Feng, Guohui; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China; [Shang, Hanqiao; Feng, Guohui] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
资助者: National Natural Science Foundation of China[30671602, 30871442]; National Basic Research Program of China[2012CB944500]; National High-tech RD (863) Program[2007AA10Z164]
摘要: Ribonucleotide reductase (RNR) is the rate-limiting enzyme in the de novo synthesis of deoxyribonucleoside triphosphates. Expression of RNR subunits is closely associated with DNA replication and repair. Mammalian RNR M2 subunit (R2) functions exclusively in DNA replication of normal cells due to its S phase-specific expression and late mitotic degradation. Herein, we demonstrate the control of R2 expression through alternative promoters, splicing and polyadenylation sites in zebrafish. Three functional R2 promoters were identified to generate six transcript variants with distinct 5' termini. The proximal promoter contains a conserved E2F binding site and two CCAAT boxes, which are crucial for the transcription of R2 gene during cell cycle. Activity of the distal promoter can be induced by DNA damage to generate four transcript variants through alternative splicing. In addition, two novel splice variants were found to encode distinct N-truncated R2 isoforms containing residues for enzymatic activity but no KEN box essential for its proteolysis. These two N-truncated R2 isoforms remained in the cytoplasm and were able to interact with RNR M1 subunit (R1). Thus, our results suggest that multilayered mechanisms control the differential expression and function of zebrafish R2 gene during cell cycle and under genotoxic stress.
语种: 英语
WOS记录号: WOS:000295832000093
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16467
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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Shang, Hanqiao; Li, Qing; Feng, Guohui; Cui, Zongbin.Identification and Characterization of Alternative Promoters, Transcripts and Protein Isoforms of Zebrafish R2 Gene,PLOS ONE,2011,6(8):e24089
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文件名: Identification and Characterization of Alternative Promoters, Transcripts and Protein Isoforms of Zebrafish R2 Gene.pdf
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