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Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19a and b) genes in zebrafish (Danio rerio) larvae
Wang, Jingxian; Shi, Xiongjie; Du, Yongbin; Zhou, Bingsheng; Wang, JX (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; wangjx@ihb.ac.cn
2011
Source PublicationJOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
ISSN1093-4529
Volume46Issue:9Pages:960-967
AbstractIn the present study, expression levels of vitellogenin (vtg) and cytochrome P450 aromatase genes (cyp19a and cyp19b) in zebrafish larvae during the early stages of development were investigated by quantitative real time-PCR assay. The results indicated that vtg gene transcription was induced seven days after zebrafish larvae fertilization, whereas the expression of cyp19a and cyp19b genes was detected as early as 3 and 4 days post-fertilization (dpf). Investigations into the effects of 17 beta-estradiol (E2) exposure on the expression of these genes showed that both vtg and cyp19b were upregulated by E2 in zebrafish larvae as early as four dpf, whereas no variation was observed in cyp19a gene expression. The estrogenic potential of pharmaceutical estrogen (DES), phenol estrogen (BPA) and the brominated flame retardants, TBBPA, DE-71 and 4-BP, were evaluated by analyzing the expression of these three genes in zebrafish larvae. The results demonstrated that natural estrogen, endocrine disrupting compounds and brominated flame retardants act as endocrine disrupters through different mechanisms. We have demonstrated for the first time that the polybrominated diphenyl ether mixture, DE-71, acts as an endocrine disrupter by upregulation of cyp19b gene expression at a relatively low concentration. These results indicate that analysis of vtg and cyp19b gene expression in zebrafish during early embryogenesis and organogenesis represents the basis of a sensitive and fast bioassay for the routine assessment of xenoestrogen effects.
KeywordEndocrine Disrupter Chemicals Brominated Flame Retardant Vitellogenin Aromatase Genes Cyp19a/b Zebrafish Larvae
Department[Wang, Jingxian; Shi, Xiongjie; Du, Yongbin; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Subject AreaEngineering ; Environmental Sciences & Ecology
Funding OrganizationNational Natural Science Foundation of China[20890113]; State Key Laboratory Foundation; Chinese Academy of Sciences[2008FB013]; Natural Science Foundation of Hubei Province, China[2007ABA215] ; National Natural Science Foundation of China[20890113]; State Key Laboratory Foundation; Chinese Academy of Sciences[2008FB013]; Natural Science Foundation of Hubei Province, China[2007ABA215] ; National Natural Science Foundation of China[20890113]; State Key Laboratory Foundation; Chinese Academy of Sciences[2008FB013]; Natural Science Foundation of Hubei Province, China[2007ABA215] ; National Natural Science Foundation of China[20890113]; State Key Laboratory Foundation; Chinese Academy of Sciences[2008FB013]; Natural Science Foundation of Hubei Province, China[2007ABA215]
Indexed BySCI
Language英语
WOS IDWOS:000293834700006
Funding OrganizationNational Natural Science Foundation of China[20890113]; State Key Laboratory Foundation; Chinese Academy of Sciences[2008FB013]; Natural Science Foundation of Hubei Province, China[2007ABA215] ; National Natural Science Foundation of China[20890113]; State Key Laboratory Foundation; Chinese Academy of Sciences[2008FB013]; Natural Science Foundation of Hubei Province, China[2007ABA215] ; National Natural Science Foundation of China[20890113]; State Key Laboratory Foundation; Chinese Academy of Sciences[2008FB013]; Natural Science Foundation of Hubei Province, China[2007ABA215] ; National Natural Science Foundation of China[20890113]; State Key Laboratory Foundation; Chinese Academy of Sciences[2008FB013]; Natural Science Foundation of Hubei Province, China[2007ABA215]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16461
Collection水环境工程研究中心_期刊论文
Corresponding AuthorWang, JX (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; wangjx@ihb.ac.cn
Recommended Citation
GB/T 7714
Wang, Jingxian,Shi, Xiongjie,Du, Yongbin,et al. Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19a and b) genes in zebrafish (Danio rerio) larvae[J]. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING,2011,46(9):960-967.
APA Wang, Jingxian,Shi, Xiongjie,Du, Yongbin,Zhou, Bingsheng,Wang, JX ,&wangjx@ihb.ac.cn.(2011).Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19a and b) genes in zebrafish (Danio rerio) larvae.JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING,46(9),960-967.
MLA Wang, Jingxian,et al."Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19a and b) genes in zebrafish (Danio rerio) larvae".JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING 46.9(2011):960-967.
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