IHB OpenIR  > 水环境工程研究中心  > 期刊论文
Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples
Fang, Huaifang; Yang, Fangxing; Sun, Junling; Tian, Yun; Zeng, Zhaorui; Xu, Ying; Zeng, ZR (reprint author), Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China; zrzeng@whu.edu.cn
2011
Source PublicationTALANTA
ISSN0039-9140
Volume85Issue:4Pages:2148-2153
AbstractA novel method that combines two concentration techniques, off-line centrifuge microextraction (CME) and on-line sweeping, is used to determine trace steroids in urine by micellar electrokinetic chromatography (MEKC). The CME-sweeping-MEKC is a promising technique, which has a variety of merits such as high sensitivity, rapid operation, minimal cost, and high sample throughput capability. Using CME-sweeping, over a 500-fold increase in sensitivity could be obtained as compared with the normal hydrodynamic injection without sample stacking. The linear range was 0.05-1 mu g mL(-1) with the square of the correlation coefficients ranging from 0.9998 to 0.9999. Detection limits (S/N = 3) were 5-15 ng mL(-1) using a photodiode array UV detection at a wavelength of 240 nm. The relative standard deviations were between 2.8% and 3.5% (n = 5, progesterone as the internal standard). The diode array UV spectrum used can distinguish the analytes from the interference in the complex sample matrix, which is useful in biological and clinical sample analysis. (C) 2011 Elsevier B.V. All rights reserved.
KeywordCentrifuge Microextraction Sweeping Micellar Electrokinetic Chromatography Steroid Hormone
Department[Fang, Huaifang; Sun, Junling; Tian, Yun; Zeng, Zhaorui] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China; [Yang, Fangxing; Xu, Ying] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaChemistry
Funding OrganizationNational Natural Science Foundation of China[20375028]; National Basic Research Program, China[2003CB415005]; Chinese Academy of Sciences[KSCX2-SW-128] ; National Natural Science Foundation of China[20375028]; National Basic Research Program, China[2003CB415005]; Chinese Academy of Sciences[KSCX2-SW-128] ; National Natural Science Foundation of China[20375028]; National Basic Research Program, China[2003CB415005]; Chinese Academy of Sciences[KSCX2-SW-128] ; National Natural Science Foundation of China[20375028]; National Basic Research Program, China[2003CB415005]; Chinese Academy of Sciences[KSCX2-SW-128]
Indexed BySCI
Language英语
WOS IDWOS:000295393000066
Funding OrganizationNational Natural Science Foundation of China[20375028]; National Basic Research Program, China[2003CB415005]; Chinese Academy of Sciences[KSCX2-SW-128] ; National Natural Science Foundation of China[20375028]; National Basic Research Program, China[2003CB415005]; Chinese Academy of Sciences[KSCX2-SW-128] ; National Natural Science Foundation of China[20375028]; National Basic Research Program, China[2003CB415005]; Chinese Academy of Sciences[KSCX2-SW-128] ; National Natural Science Foundation of China[20375028]; National Basic Research Program, China[2003CB415005]; Chinese Academy of Sciences[KSCX2-SW-128]
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16457
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZeng, ZR (reprint author), Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China; zrzeng@whu.edu.cn
Recommended Citation
GB/T 7714
Fang, Huaifang,Yang, Fangxing,Sun, Junling,et al. Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples[J]. TALANTA,2011,85(4):2148-2153.
APA Fang, Huaifang.,Yang, Fangxing.,Sun, Junling.,Tian, Yun.,Zeng, Zhaorui.,...&zrzeng@whu.edu.cn.(2011).Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples.TALANTA,85(4),2148-2153.
MLA Fang, Huaifang,et al."Centrifuge microextraction coupled with sweeping-MEKC to analyze trace steroid hormones in urine samples".TALANTA 85.4(2011):2148-2153.
Files in This Item:
File Name/Size DocType Version Access License
Centrifuge microextr(544KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Fang, Huaifang]'s Articles
[Yang, Fangxing]'s Articles
[Sun, Junling]'s Articles
Baidu academic
Similar articles in Baidu academic
[Fang, Huaifang]'s Articles
[Yang, Fangxing]'s Articles
[Sun, Junling]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Fang, Huaifang]'s Articles
[Yang, Fangxing]'s Articles
[Sun, Junling]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Centrifuge microextraction coupled with sweeping-MEKC.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.