IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization
Zhu, Hua Ping; Lu, Mai Xin; Huang, Zhang Han; Gao, Feng Ying; Ma, Dong Mei; Zhou, Li; Gui, Jian Fang; Lu, MX (reprint author), Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China; mx-lu@163.com
2011
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Volume42Issue:8Pages:1178-1185
AbstractChromosomal karyotypes of Oreochromis mossambicus and O. urolepis hornorum and their hybrid were analysed by means of Cot-1 DNA bandings through fluorescence in situ hybridization (FISH). To identify all chromosomes, Cot-1DNA - which contains highly and moderately repetitive DNA - was extracted from genomic DNA, labelled as a probe with Dig-11-dUTP, and in situ hybridized to spreads of mitotic chromosomes of the three samples. The hybridized signals were detected by means of Cy3-conjugated antidigoxigenin. The FISH results indicated that the three samples had the same diploid number (2n = 44) of chromosomes. Specific fluorescence signal bands were detected on all individual chromosome pairs. On the basis of Cot-1 DNA FISH banding patterns and chromosome morphology, the karyotypes of the three samples have been constructed; no remarkable differences were detected between the karyotypes of these species using this method. These results - which are similar to those reported previously, with respect to chromosome number, morphology and Cot-1DNA FISH patterns - suggest chromosomal stasis during speciation and hybridization of tilapia (Oreochromis, Cichlidae). Such a molecular cytogenetic procedure, if used in conjunction with other genomic research methods, could facilitate the study of genomic structure and be adapted for chromosome studies of other animal species.
KeywordTilapia Karyotype Cot-1 Dna Fluorescence In Situ Hybridization (Fish)
Department[Zhu, Hua Ping; Lu, Mai Xin; Huang, Zhang Han; Gao, Feng Ying; Ma, Dong Mei] Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China; [Zhou, Li; Gui, Jian Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
Subject AreaFisheries
Funding OrganizationSpecial Scientific Research Fund for Commonwealth Agricultural Industry of China[3-49]; Key Program for Agriculture Research of Guangdong Province[2009B020201003]; Natural Science Foundation of Guangdong Province[9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-48-4]; Marine Fisheries Science and Technology Promotion Project of Guangdong Province[A200901C01]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology of China[2009FB03] ; Special Scientific Research Fund for Commonwealth Agricultural Industry of China[3-49]; Key Program for Agriculture Research of Guangdong Province[2009B020201003]; Natural Science Foundation of Guangdong Province[9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-48-4]; Marine Fisheries Science and Technology Promotion Project of Guangdong Province[A200901C01]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology of China[2009FB03] ; Special Scientific Research Fund for Commonwealth Agricultural Industry of China[3-49]; Key Program for Agriculture Research of Guangdong Province[2009B020201003]; Natural Science Foundation of Guangdong Province[9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-48-4]; Marine Fisheries Science and Technology Promotion Project of Guangdong Province[A200901C01]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology of China[2009FB03] ; Special Scientific Research Fund for Commonwealth Agricultural Industry of China[3-49]; Key Program for Agriculture Research of Guangdong Province[2009B020201003]; Natural Science Foundation of Guangdong Province[9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-48-4]; Marine Fisheries Science and Technology Promotion Project of Guangdong Province[A200901C01]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology of China[2009FB03]
Indexed BySCI
Language英语
WOS IDWOS:000292650100014
Funding OrganizationSpecial Scientific Research Fund for Commonwealth Agricultural Industry of China[3-49]; Key Program for Agriculture Research of Guangdong Province[2009B020201003]; Natural Science Foundation of Guangdong Province[9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-48-4]; Marine Fisheries Science and Technology Promotion Project of Guangdong Province[A200901C01]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology of China[2009FB03] ; Special Scientific Research Fund for Commonwealth Agricultural Industry of China[3-49]; Key Program for Agriculture Research of Guangdong Province[2009B020201003]; Natural Science Foundation of Guangdong Province[9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-48-4]; Marine Fisheries Science and Technology Promotion Project of Guangdong Province[A200901C01]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology of China[2009FB03] ; Special Scientific Research Fund for Commonwealth Agricultural Industry of China[3-49]; Key Program for Agriculture Research of Guangdong Province[2009B020201003]; Natural Science Foundation of Guangdong Province[9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-48-4]; Marine Fisheries Science and Technology Promotion Project of Guangdong Province[A200901C01]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology of China[2009FB03] ; Special Scientific Research Fund for Commonwealth Agricultural Industry of China[3-49]; Key Program for Agriculture Research of Guangdong Province[2009B020201003]; Natural Science Foundation of Guangdong Province[9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-48-4]; Marine Fisheries Science and Technology Promotion Project of Guangdong Province[A200901C01]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology of China[2009FB03]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16445
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLu, MX (reprint author), Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China; mx-lu@163.com
Recommended Citation
GB/T 7714
Zhu, Hua Ping,Lu, Mai Xin,Huang, Zhang Han,et al. Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization[J]. AQUACULTURE RESEARCH,2011,42(8):1178-1185.
APA Zhu, Hua Ping.,Lu, Mai Xin.,Huang, Zhang Han.,Gao, Feng Ying.,Ma, Dong Mei.,...&mx-lu@163.com.(2011).Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization.AQUACULTURE RESEARCH,42(8),1178-1185.
MLA Zhu, Hua Ping,et al."Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization".AQUACULTURE RESEARCH 42.8(2011):1178-1185.
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