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学科主题: Fisheries
题名: Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization
作者: Zhu, Hua Ping ; Lu, Mai Xin ; Huang, Zhang Han ; Gao, Feng Ying ; Ma, Dong Mei ; Zhou, Li ; Gui, Jian Fang
通讯作者: Lu, MX (reprint author), Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China ; mx-lu@163.com
关键词: tilapia ; karyotype ; Cot-1 DNA ; fluorescence in situ hybridization (FISH)
刊名: AQUACULTURE RESEARCH
发表日期: 2011
卷: 42, 期:8, 页:1178-1185
收录类别: SCI
部门归属: [Zhu, Hua Ping; Lu, Mai Xin; Huang, Zhang Han; Gao, Feng Ying; Ma, Dong Mei] Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China; [Zhou, Li; Gui, Jian Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
资助者: Special Scientific Research Fund for Commonwealth Agricultural Industry of China[3-49]; Key Program for Agriculture Research of Guangdong Province[2009B020201003]; Natural Science Foundation of Guangdong Province[9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-48-4]; Marine Fisheries Science and Technology Promotion Project of Guangdong Province[A200901C01]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology of China[2009FB03]
摘要: Chromosomal karyotypes of Oreochromis mossambicus and O. urolepis hornorum and their hybrid were analysed by means of Cot-1 DNA bandings through fluorescence in situ hybridization (FISH). To identify all chromosomes, Cot-1DNA - which contains highly and moderately repetitive DNA - was extracted from genomic DNA, labelled as a probe with Dig-11-dUTP, and in situ hybridized to spreads of mitotic chromosomes of the three samples. The hybridized signals were detected by means of Cy3-conjugated antidigoxigenin. The FISH results indicated that the three samples had the same diploid number (2n = 44) of chromosomes. Specific fluorescence signal bands were detected on all individual chromosome pairs. On the basis of Cot-1 DNA FISH banding patterns and chromosome morphology, the karyotypes of the three samples have been constructed; no remarkable differences were detected between the karyotypes of these species using this method. These results - which are similar to those reported previously, with respect to chromosome number, morphology and Cot-1DNA FISH patterns - suggest chromosomal stasis during speciation and hybridization of tilapia (Oreochromis, Cichlidae). Such a molecular cytogenetic procedure, if used in conjunction with other genomic research methods, could facilitate the study of genomic structure and be adapted for chromosome studies of other animal species.
语种: 英语
WOS记录号: WOS:000292650100014
ISSN号: 1355-557X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16445
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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Recommended Citation:
Zhu, Hua Ping; Lu, Mai Xin; Huang, Zhang Han; Gao, Feng Ying; Ma, Dong Mei; Zhou, Li; Gui, Jian Fang.Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization,AQUACULTURE RESEARCH,2011,42(8):1178-1185
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文件名: Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization.pdf
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