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An Apo-14 Promoter-Driven Transgenic Zebrafish That Marks Liver Organogenesis
Wang, Rui; Li, Zhi; Wang, Yang; Gui, Jian-Fang; Wang, R (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China; jfgui@ihb.ac.cn
2011
Source PublicationPLOS ONE
ISSN1932-6203
Volume6Issue:7Pages:e22555
AbstractSeveral transgenic zebrafish lines for liver development studies had been obtained in the first decade of this century, but not any transgenic GFP zebrafish lines that mark the through liver development and organogenesis were reported. In this study, we analyzed expression pattern of endogenous Apo-14 in zebrafish embryogenesis by whole-mount in situ hybridization, and revealed its expression in liver primordium and in the following liver development. Subsequently, we isolated zebrafish Apo-14 promoter of 1763 bp 5'-flanking sequence, and developed an Apo-14 promoter-driven transgenic zebrafish Tg(Apo14: GFP). And, maternal expression and post-fertilization translocation of Apo-14 promoter-driven GFP were observed in the transgenic zebrafish line. Moreover, we traced onset expression of Apo-14 promoter-driven GFP and developmental behavior of the expressed cells in early heterozygous embryos by out-crossing the Tg(Apo14: GFP) male to the wild type female. Significantly, the Apo-14 promoter-driven GFP is initially expressed around YSL beneath the embryo body at 10 hpf when the embryos develop to tail bud prominence. In about 14-somite embryos at 16-17 hpf, a typical "salt-and-pepper" expression pattern is clearly observed in YSL around the yolk sac. Then, a green fluorescence dot begins to appear between the notochord and the yolk sac adjacent to otic vesicle at about 20 hpf, which is later demonstrated to be liver primordium that gives rise to liver. Furthermore, we investigated dynamic progression of liver organogenesis in the Tg(Apo14: GFP) zebrafish, because the Apo-14 promoter-driven GFP is sustainably expressed from hepatoblasts and liver progenitor cells in liver primordium to hepatocytes in the larval and adult liver. Additionally, we observed similar morphology between the liver progenitor cells and the GFP-positive nuclei on the YSL, suggesting that they might originate from the same progenitor cells in early embryos. Overall, the current study provides a transgenic zebrafish line that marks the through liver organogenesis.
KeywordDigestive-system Expression Pattern Spotted Grouper Apolipoprotein Morphogenesis Fish Endoderm Domains Genes Loci
Department[Wang, Rui; Li, Zhi; Wang, Yang; Gui, Jian-Fang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China; [Wang, Rui; Li, Zhi; Wang, Yang; Gui, Jian-Fang] Wuhan Ctr Dev Biol, Wuhan, Peoples R China; [Wang, Rui] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Subject AreaLife Sciences & Biomedicine - Other Topics
Funding OrganizationNational Key Basic Research Program[2010CB126301]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ15, 2008FBZ18, 085B181F01]; Institute of Hydrobiology, Chinese Academy of Sciences[075A011301, 085A011301] ; National Key Basic Research Program[2010CB126301]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ15, 2008FBZ18, 085B181F01]; Institute of Hydrobiology, Chinese Academy of Sciences[075A011301, 085A011301] ; National Key Basic Research Program[2010CB126301]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ15, 2008FBZ18, 085B181F01]; Institute of Hydrobiology, Chinese Academy of Sciences[075A011301, 085A011301] ; National Key Basic Research Program[2010CB126301]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ15, 2008FBZ18, 085B181F01]; Institute of Hydrobiology, Chinese Academy of Sciences[075A011301, 085A011301]
Indexed BySCI
Language英语
WOS IDWOS:000293097300065
Funding OrganizationNational Key Basic Research Program[2010CB126301]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ15, 2008FBZ18, 085B181F01]; Institute of Hydrobiology, Chinese Academy of Sciences[075A011301, 085A011301] ; National Key Basic Research Program[2010CB126301]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ15, 2008FBZ18, 085B181F01]; Institute of Hydrobiology, Chinese Academy of Sciences[075A011301, 085A011301] ; National Key Basic Research Program[2010CB126301]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ15, 2008FBZ18, 085B181F01]; Institute of Hydrobiology, Chinese Academy of Sciences[075A011301, 085A011301] ; National Key Basic Research Program[2010CB126301]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ15, 2008FBZ18, 085B181F01]; Institute of Hydrobiology, Chinese Academy of Sciences[075A011301, 085A011301]
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16441
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, R (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China; jfgui@ihb.ac.cn
Recommended Citation
GB/T 7714
Wang, Rui,Li, Zhi,Wang, Yang,et al. An Apo-14 Promoter-Driven Transgenic Zebrafish That Marks Liver Organogenesis[J]. PLOS ONE,2011,6(7):e22555.
APA Wang, Rui,Li, Zhi,Wang, Yang,Gui, Jian-Fang,Wang, R ,&jfgui@ihb.ac.cn.(2011).An Apo-14 Promoter-Driven Transgenic Zebrafish That Marks Liver Organogenesis.PLOS ONE,6(7),e22555.
MLA Wang, Rui,et al."An Apo-14 Promoter-Driven Transgenic Zebrafish That Marks Liver Organogenesis".PLOS ONE 6.7(2011):e22555.
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