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学科主题: Virology
题名: Cooperative Roles of Fish Protein Kinase Containing Z-DNA Binding Domains and Double-Stranded RNA-Dependent Protein Kinase in Interferon-Mediated Antiviral Response
作者: Liu, Ting-Kai ; Zhang, Yi-Bing ; Liu, Ying ; Sun, Fan ; Gui, Jian-Fang
通讯作者: Zhang, YB (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China ; ybzhang@ihb.ac.cn ; jfgui@ihb.ac.cn
关键词: INNATE IMMUNE-RESPONSE ; UV-INACTIVATED VIRUS ; EUKARYOTIC INITIATION-FACTOR-2-ALPHA ; TYROSINE PHOSPHORYLATION ; SUBSTRATE RECOGNITION ; ADENOSINE-DEAMINASE ; MOLECULAR-CLONING ; CAB CELLS ; IN-VIVO ; PKR
刊名: JOURNAL OF VIROLOGY
发表日期: 2011
卷: 85, 期:23, 页:12769-12780
收录类别: SCI
部门归属: [Liu, Ting-Kai; Zhang, Yi-Bing; Liu, Ying; Sun, Fan; Gui, Jian-Fang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China
资助者: 973 National Basic Research Program of China[2010CB126301, 2010CB126303]; National Natural Science Foundation of China[30871922]; national transgenic project[2009ZX08010-021B]
摘要: The double-stranded RNA (dsRNA)-dependent protein kinase (PKR) inhibits protein synthesis by phosphorylating eukaryotic translation initiation factor 2 alpha (eIF2 alpha). In fish species, in addition to PKR, there exists a PKR-like protein kinase containing Z-DNA binding domains (PKZ). However, the antiviral role of fish PKZ and the functional relationship between fish PKZ and PKR remain unknown. Here we confirmed the coexpression of fish PKZ and PKR proteins in Carassius auratus blastula embryonic (CAB) cells and identified them as two typical interferon (IFN)-inducible eIF2 alpha kinases, both of which displayed an ability to inhibit virus replication. Strikingly, fish IFN or all kinds of IFN stimuli activated PKZ and PKR to phosphorylated eIF2 alpha. Overexpression of both fish kinases together conferred much more significant inhibition of virus replication than overexpression of either protein, whereas morpholino knockdown of both made fish cells more vulnerable to virus infection than knockdown of either. The antiviral ability of fish PKZ was weaker than fish PKR, which correlated with its lower ability to phosphorylate eIF2 alpha than PKR. Moreover, the independent association of fish PKZ or PKR reveals that each of them formed homodimers and that fish PKZ phosphorylated eIF2 alpha independently on fish PKR and vice versa. These results suggest that fish PKZ and PKR play a nonredundant but cooperative role in IFN antiviral response.
语种: 英语
WOS记录号: WOS:000296708100063
ISSN号: 0022-538X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16437
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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Recommended Citation:
Liu, Ting-Kai; Zhang, Yi-Bing; Liu, Ying; Sun, Fan; Gui, Jian-Fang.Cooperative Roles of Fish Protein Kinase Containing Z-DNA Binding Domains and Double-Stranded RNA-Dependent Protein Kinase in Interferon-Mediated Antiviral Response,JOURNAL OF VIROLOGY,2011,85(23):12769-12780
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文件名: Cooperative Roles of Fish Protein Kinase Containing Z-DNA Binding Domains and Double-Stranded RNA-Dependent Protein Kinase in Interferon-Mediated Antiviral Response.pdf
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