IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Cooperative Roles of Fish Protein Kinase Containing Z-DNA Binding Domains and Double-Stranded RNA-Dependent Protein Kinase in Interferon-Mediated Antiviral Response
Liu, Ting-Kai; Zhang, Yi-Bing; Liu, Ying; Sun, Fan; Gui, Jian-Fang; Zhang, YB (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China; ybzhang@ihb.ac.cn; jfgui@ihb.ac.cn
2011
Source PublicationJOURNAL OF VIROLOGY
ISSN0022-538X
Volume85Issue:23Pages:12769-12780
AbstractThe double-stranded RNA (dsRNA)-dependent protein kinase (PKR) inhibits protein synthesis by phosphorylating eukaryotic translation initiation factor 2 alpha (eIF2 alpha). In fish species, in addition to PKR, there exists a PKR-like protein kinase containing Z-DNA binding domains (PKZ). However, the antiviral role of fish PKZ and the functional relationship between fish PKZ and PKR remain unknown. Here we confirmed the coexpression of fish PKZ and PKR proteins in Carassius auratus blastula embryonic (CAB) cells and identified them as two typical interferon (IFN)-inducible eIF2 alpha kinases, both of which displayed an ability to inhibit virus replication. Strikingly, fish IFN or all kinds of IFN stimuli activated PKZ and PKR to phosphorylated eIF2 alpha. Overexpression of both fish kinases together conferred much more significant inhibition of virus replication than overexpression of either protein, whereas morpholino knockdown of both made fish cells more vulnerable to virus infection than knockdown of either. The antiviral ability of fish PKZ was weaker than fish PKR, which correlated with its lower ability to phosphorylate eIF2 alpha than PKR. Moreover, the independent association of fish PKZ or PKR reveals that each of them formed homodimers and that fish PKZ phosphorylated eIF2 alpha independently on fish PKR and vice versa. These results suggest that fish PKZ and PKR play a nonredundant but cooperative role in IFN antiviral response.
KeywordInnate Immune-response Uv-inactivated Virus Eukaryotic Initiation-factor-2-alpha Tyrosine Phosphorylation Substrate Recognition Adenosine-deaminase Molecular-cloning Cab Cells In-vivo Pkr
Department[Liu, Ting-Kai; Zhang, Yi-Bing; Liu, Ying; Sun, Fan; Gui, Jian-Fang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China
Subject AreaVirology
Funding Organization973 National Basic Research Program of China[2010CB126301, 2010CB126303]; National Natural Science Foundation of China[30871922]; national transgenic project[2009ZX08010-021B] ; 973 National Basic Research Program of China[2010CB126301, 2010CB126303]; National Natural Science Foundation of China[30871922]; national transgenic project[2009ZX08010-021B] ; 973 National Basic Research Program of China[2010CB126301, 2010CB126303]; National Natural Science Foundation of China[30871922]; national transgenic project[2009ZX08010-021B] ; 973 National Basic Research Program of China[2010CB126301, 2010CB126303]; National Natural Science Foundation of China[30871922]; national transgenic project[2009ZX08010-021B]
Indexed BySCI
Language英语
WOS IDWOS:000296708100063
Funding Organization973 National Basic Research Program of China[2010CB126301, 2010CB126303]; National Natural Science Foundation of China[30871922]; national transgenic project[2009ZX08010-021B] ; 973 National Basic Research Program of China[2010CB126301, 2010CB126303]; National Natural Science Foundation of China[30871922]; national transgenic project[2009ZX08010-021B] ; 973 National Basic Research Program of China[2010CB126301, 2010CB126303]; National Natural Science Foundation of China[30871922]; national transgenic project[2009ZX08010-021B] ; 973 National Basic Research Program of China[2010CB126301, 2010CB126303]; National Natural Science Foundation of China[30871922]; national transgenic project[2009ZX08010-021B]
Citation statistics
Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16437
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, YB (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China; ybzhang@ihb.ac.cn; jfgui@ihb.ac.cn
Recommended Citation
GB/T 7714
Liu, Ting-Kai,Zhang, Yi-Bing,Liu, Ying,et al. Cooperative Roles of Fish Protein Kinase Containing Z-DNA Binding Domains and Double-Stranded RNA-Dependent Protein Kinase in Interferon-Mediated Antiviral Response[J]. JOURNAL OF VIROLOGY,2011,85(23):12769-12780.
APA Liu, Ting-Kai.,Zhang, Yi-Bing.,Liu, Ying.,Sun, Fan.,Gui, Jian-Fang.,...&jfgui@ihb.ac.cn.(2011).Cooperative Roles of Fish Protein Kinase Containing Z-DNA Binding Domains and Double-Stranded RNA-Dependent Protein Kinase in Interferon-Mediated Antiviral Response.JOURNAL OF VIROLOGY,85(23),12769-12780.
MLA Liu, Ting-Kai,et al."Cooperative Roles of Fish Protein Kinase Containing Z-DNA Binding Domains and Double-Stranded RNA-Dependent Protein Kinase in Interferon-Mediated Antiviral Response".JOURNAL OF VIROLOGY 85.23(2011):12769-12780.
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