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Analysis of MicroRNA Expression in Embryonic Developmental Toxicity Induced by MC-RR
Zhao, Yanyan; Xiong, Qian; Xie, Ping; Zhao, YY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosys, Wuhan, Peoples R China; xieping@ihb.ac.cn
2011
Source PublicationPLOS ONE
ISSN1932-6203
Volume6Issue:7Pages:e22676
AbstractAs cynobacterial blooms frequently occur in fresh waters throughout the world, microcystins (MCs) have caused serious damage to both wildlife and human health. MCs are known to have developmental toxicity, however, the possible molecular mechanism is largely unknown. This is the first toxicological study to integrate post-transcriptomic, proteomic and bioinformatics analysis to explore molecular mechanisms for developmental toxicity of MCs in zebrafish. After being microinjected directly into embryos, MC-RR dose-dependently decreased survival rates and increased malformation rates of embryos, causing various embryo abnormalities including loss of vascular integrity and hemorrhage. Expressions of 31 microRNAs (miRNAs) and 78 proteins were significantly affected at 72 hours post-fertilisation (hpf). Expressions of miR-430 and miR-125 families were also significantly changed. The altered expressions of miR-31 and miR-126 were likely responsible for the loss of vascular integrity. MC-RR significantly reduced the expressions of a number of proteins involved in energy metabolism, cell division, protein synthesis, cytoskeleton maintenance, response to stress and DNA replication. Bioinformatics analysis shows that several aberrantly expressed miRNAs and proteins (involved in various molecular pathways) were predicted to be potential MC-responsive miRNA-target pairs, and that their aberrant expressions should be the possible molecular mechanisms for the various developmental defects caused by MC-RR.
KeywordCyprinus-carpio L. Microcystin-lr Gene-expression Zebrafish Development Negative Regulator Rna-interference Danio-rerio Cyanobacteria Dicer Extract
Department[Zhao, Yanyan; Xiong, Qian; Xie, Ping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosys, Wuhan, Peoples R China; [Zhao, Yanyan; Xiong, Qian] Chinese Acad Sci, Grad Sch, Wuhan, Peoples R China
Subject AreaLife Sciences & Biomedicine - Other Topics
Funding OrganizationNational Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]
Indexed BySCI
Language英语
WOS IDWOS:000293286500017
Funding OrganizationNational Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457] ; National Basic Research Program of China (973 Program)[2008CB418101]; National Natural Science Foundation of China[31070457]
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Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16431
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorZhao, YY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosys, Wuhan, Peoples R China; xieping@ihb.ac.cn
Recommended Citation
GB/T 7714
Zhao, Yanyan,Xiong, Qian,Xie, Ping,et al. Analysis of MicroRNA Expression in Embryonic Developmental Toxicity Induced by MC-RR[J]. PLOS ONE,2011,6(7):e22676.
APA Zhao, Yanyan,Xiong, Qian,Xie, Ping,Zhao, YY ,&xieping@ihb.ac.cn.(2011).Analysis of MicroRNA Expression in Embryonic Developmental Toxicity Induced by MC-RR.PLOS ONE,6(7),e22676.
MLA Zhao, Yanyan,et al."Analysis of MicroRNA Expression in Embryonic Developmental Toxicity Induced by MC-RR".PLOS ONE 6.7(2011):e22676.
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