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Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus
Jiang, Wenbo; Yang, Yuhui; Zhao, Dongmei; Liu, Xiaoyan; Duan, Jundan; Xie, Songguang; Zhao, Haobin; Zhao, HB (reprint author), Huazhong Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China; zhaohb@mail.ccnu.edu.cn
2011
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume160Issue:4Pages:187-193
AbstractGobiocypris rams is an emerging fish model for aquatic toxicology in China as it is sensitive to environmental hormone disruptors. Exogenous sex steroids can affect sex differentiation and the expression of sex-related genes. Foxl2, a member of forkhead-box transcription factor family, is the key gene for ovary development and its mutation causes the blepharophimosis ptosis epicanthus inversus syndrome in human. We find that two foxl2 genes exist in fish genome, one is foxl2, and the other is foxl2b. Here, we reported the isolation and expression of foxl2 in G. rams. G. rarus foxl2 cDNA is 1700 bp in length with a 921 bp of open reading frame encoding 306 amino acids containing the typical FM-domain. Semi-quantitative RT-PCR revealed its predominant expression in the eye, brain, gill and gonads. Moreover, the expression level in the ovary was significantly higher than that in the testis. Quantitative RT-PCR showed that foxl2 was up regulated after treatment with estradiol and was down regulated with 2-methyl-testosterone. These results suggested that Foxl2 plays an important role in female development of G. rams, foxl2 mRNA expression is regulated by downstream sex hormones, and foxl2 can be used as a molecular indicator monitoring the environmental endocrine disruptors. (C) 2011 Elsevier Inc. All rights reserved.
KeywordGobiocypris Rarus Sex Determination And Sex Differentiation Foxl2 Foxl2b Expression Pattern
Department[Jiang, Wenbo; Yang, Yuhui; Zhao, Dongmei; Liu, Xiaoyan; Duan, Jundan; Zhao, Haobin] Huazhong Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China; [Xie, Songguang] Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Aquat Biodivers & Resource Conservat, Wuhan 430072, Hubei, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Zoology
Funding OrganizationNational Key Laboratory of Aquatic Diversity and Resource Conservation; Hubei Key Laboratory of Genetic Regulation and Integrative Biology ; National Key Laboratory of Aquatic Diversity and Resource Conservation; Hubei Key Laboratory of Genetic Regulation and Integrative Biology ; National Key Laboratory of Aquatic Diversity and Resource Conservation; Hubei Key Laboratory of Genetic Regulation and Integrative Biology ; National Key Laboratory of Aquatic Diversity and Resource Conservation; Hubei Key Laboratory of Genetic Regulation and Integrative Biology
Indexed BySCI
Language英语
WOS IDWOS:000296679800007
Funding OrganizationNational Key Laboratory of Aquatic Diversity and Resource Conservation; Hubei Key Laboratory of Genetic Regulation and Integrative Biology ; National Key Laboratory of Aquatic Diversity and Resource Conservation; Hubei Key Laboratory of Genetic Regulation and Integrative Biology ; National Key Laboratory of Aquatic Diversity and Resource Conservation; Hubei Key Laboratory of Genetic Regulation and Integrative Biology ; National Key Laboratory of Aquatic Diversity and Resource Conservation; Hubei Key Laboratory of Genetic Regulation and Integrative Biology
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Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16411
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorZhao, HB (reprint author), Huazhong Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China; zhaohb@mail.ccnu.edu.cn
Recommended Citation
GB/T 7714
Jiang, Wenbo,Yang, Yuhui,Zhao, Dongmei,et al. Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2011,160(4):187-193.
APA Jiang, Wenbo.,Yang, Yuhui.,Zhao, Dongmei.,Liu, Xiaoyan.,Duan, Jundan.,...&zhaohb@mail.ccnu.edu.cn.(2011).Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,160(4),187-193.
MLA Jiang, Wenbo,et al."Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 160.4(2011):187-193.
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