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学科主题: Life Sciences & Biomedicine - Other Topics
题名: Characterization and Comparative Profiling of MiRNA Transcriptomes in Bighead Carp and Silver Carp
作者: Chi, Wei ; Tong, Chaobo ; Gan, Xiaoni ; He, Shunping
通讯作者: Chi, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Lab Fish Phylogenet & Biogeog, Wuhan, Hubei, Peoples R China ; clad@ihb.ac.cn
关键词: ARABIDOPSIS-THALIANA ; DUPLICATE GENES ; MICRORNA GENES ; C-ELEGANS ; EVOLUTION ; EXPRESSION ; DROSOPHILA ; RNAS ; SET ; RETROTRANSPOSONS
刊名: PLOS ONE
发表日期: 2011
卷: 6, 期:8, 页:e23549
收录类别: SCI
部门归属: [Chi, Wei; Tong, Chaobo; Gan, Xiaoni; He, Shunping] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Phylogenet & Biogeog, Wuhan, Hubei, Peoples R China; [Chi, Wei; Tong, Chaobo; Gan, Xiaoni] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
资助者: National Basic Research Program of China[2007CB411601]
摘要: MicroRNAs (miRNAs) are small non-coding RNA molecules that are processed from large 'hairpin' precursors and function as post-transcriptional regulators of target genes. Although many individual miRNAs have recently been extensively studied, there has been very little research on miRNA transcriptomes in teleost fishes. By using high throughput sequencing technology, we have identified 167 and 166 conserved miRNAs (belonging to 108 families) in bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix), respectively. We compared the expression patterns of conserved miRNAs by means of hierarchical clustering analysis and log2 ratio. Results indicated that there is not a strong correlation between sequence conservation and expression conservation, most of these miRNAs have similar expression patterns. However, high expression differences were also identified for several individual miRNAs. Several miRNA* sequences were also found in our dataset and some of them may have regulatory functions. Two computational strategies were used to identify novel miRNAs from un-annotated data in the two carps. A first strategy based on zebrafish genome, identified 8 and 22 novel miRNAs in bighead carp and silver carp, respectively. We postulate that these miRNAs should also exist in the zebrafish, but the methodologies used have not allowed for their detection. In the second strategy we obtained several carp-specific miRNAs, 31 in bighead carp and 32 in silver carp, which showed low expression. Gain and loss of family members were observed in several miRNA families, which suggests that duplication of animal miRNA genes may occur through evolutionary processes which are similar to the protein-coding genes.
语种: 英语
WOS记录号: WOS:000293953700032
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16397
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

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Chi, Wei; Tong, Chaobo; Gan, Xiaoni; He, Shunping.Characterization and Comparative Profiling of MiRNA Transcriptomes in Bighead Carp and Silver Carp,PLOS ONE,2011,6(8):e23549
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