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学科主题: Marine & Freshwater Biology
题名: Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria)
作者: Li, Jie ; Ou, Danyun ; Zheng, Lingling ; Gan, Nanqin ; Song, Lirong
通讯作者: Song, LR (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; lrsong@ihb.ac.cn
关键词: esterase activity ; fluorescein diacetate ; low temperature ; metabolic activity ; metabolic vigor ; photosynthetic activity
刊名: PHYCOLOGICAL RESEARCH
发表日期: 2011
卷: 59, 期:3, 页:200-207
收录类别: SCI
部门归属: [Li, J; Zheng, LL; Gan, NQ; Song, LR] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Li, J] Cent S Univ, Sch Biol Sci & Technol, Changsha 410013, Hunan, Peoples R China; [Ou, DY] State Ocean Adm, Inst Oceanog 3, Xiamen 361005, Peoples R China
资助者: National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1]
摘要: The fluorescein diacetate (FDA) assay has been widely used to measure metabolic activity in phytoplankton. It was found that FDA fluorescence values did not decrease in some stressed cells, demonstrating that the applicability of the method needs to be assessed further in the context of growth-influencing conditions. In the present study, changes of FDA fluorescence values were studied in bloom-forming cyanobacterial Microcystis aeruginosa Kutz cells under stress conditions such as nitrogen (N) or phosphorus (P) deficiency, or darkness and low temperature (10 degrees C), respectively. The results demonstrated that esterase activity decreased immediately in dark-stressed cells, which correlated with the decline of biomass and photosynthetic activity. Under the other three stress conditions, however, especially at low temperature, the cells lost photosynthetic activity but had the highest esterase activity, which was five times higher than the control group. These findings contrast with the assay criteria that the expression of a stain should reflect the change of photosynthetic activity and that stressed cells should have a lower staining intensity than the control cells. According to these results, the esterase activity response was dependent on environmental factors. Furthermore, higher fluorescence intensity did not mean higher metabolic activity, but a discrepant value indicated a severe stress.
语种: 英语
WOS记录号: WOS:000292157900008
ISSN号: 1322-0829
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16381
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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Recommended Citation:
Li, Jie; Ou, Danyun; Zheng, Lingling; Gan, Nanqin; Song, Lirong.Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria),PHYCOLOGICAL RESEARCH,2011,59(3):200-207
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