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Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria)
Li, Jie; Ou, Danyun; Zheng, Lingling; Gan, Nanqin; Song, Lirong; Song, LR (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; lrsong@ihb.ac.cn
2011
Source PublicationPHYCOLOGICAL RESEARCH
ISSN1322-0829
Volume59Issue:3Pages:200-207
AbstractThe fluorescein diacetate (FDA) assay has been widely used to measure metabolic activity in phytoplankton. It was found that FDA fluorescence values did not decrease in some stressed cells, demonstrating that the applicability of the method needs to be assessed further in the context of growth-influencing conditions. In the present study, changes of FDA fluorescence values were studied in bloom-forming cyanobacterial Microcystis aeruginosa Kutz cells under stress conditions such as nitrogen (N) or phosphorus (P) deficiency, or darkness and low temperature (10 degrees C), respectively. The results demonstrated that esterase activity decreased immediately in dark-stressed cells, which correlated with the decline of biomass and photosynthetic activity. Under the other three stress conditions, however, especially at low temperature, the cells lost photosynthetic activity but had the highest esterase activity, which was five times higher than the control group. These findings contrast with the assay criteria that the expression of a stain should reflect the change of photosynthetic activity and that stressed cells should have a lower staining intensity than the control cells. According to these results, the esterase activity response was dependent on environmental factors. Furthermore, higher fluorescence intensity did not mean higher metabolic activity, but a discrepant value indicated a severe stress.
KeywordEsterase Activity Fluorescein Diacetate Low Temperature Metabolic Activity Metabolic Vigor Photosynthetic Activity
Department[Li, J; Zheng, LL; Gan, NQ; Song, LR] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Li, J] Cent S Univ, Sch Biol Sci & Technol, Changsha 410013, Hunan, Peoples R China; [Ou, DY] State Ocean Adm, Inst Oceanog 3, Xiamen 361005, Peoples R China
Subject AreaMarine & Freshwater Biology
Funding OrganizationNational Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1] ; National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1] ; National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1] ; National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1]
Indexed BySCI
Language英语
WOS IDWOS:000292157900008
Funding OrganizationNational Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1] ; National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1] ; National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1] ; National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1]
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Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16381
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorSong, LR (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; lrsong@ihb.ac.cn
Recommended Citation
GB/T 7714
Li, Jie,Ou, Danyun,Zheng, Lingling,et al. Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria)[J]. PHYCOLOGICAL RESEARCH,2011,59(3):200-207.
APA Li, Jie.,Ou, Danyun.,Zheng, Lingling.,Gan, Nanqin.,Song, Lirong.,...&lrsong@ihb.ac.cn.(2011).Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria).PHYCOLOGICAL RESEARCH,59(3),200-207.
MLA Li, Jie,et al."Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria)".PHYCOLOGICAL RESEARCH 59.3(2011):200-207.
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