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学科主题: Plant Sciences
题名: Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum
作者: Zhang, Yu ; Hu, Chun-xiang ; Zhang, Cheng-gui ; Gan, Li
通讯作者: Gan, L (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China ; ganli@mail.hzau.edu.cn
关键词: Brassica napus ; cDNA-AFLP ; LRR-RI ; Northern blotting ; RT-RACE ; Sclerotinia sclerotiorum ; Semiquantitative RT-PCR
刊名: ACTA PHYSIOLOGIAE PLANTARUM
发表日期: 2011
卷: 33, 期:4, 页:1277-1283
收录类别: SCI
部门归属: [Gan, L] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China; [Zhang, Y; Hu, CX] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhang, Y] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Zhang, CG] Dali Univ, Coll Pharm, Dali 671003, Peoples R China
资助者: National Nature Science Foundation of China[30170600]; Hubei Nature Science Foundation[2001ABD113]
摘要: Based on the significant differences in the resistance to Sclerotinia sclerotiorum between two Brassica napus cultivars, ZhongR888 and Zhongyou821, near isogenic lines (NILs) were established. The resistance differences between Zhongyou821 and BC(5) progeny (the fifth generation from a cross between Zhongyou821 and ZhongR888 with Zhongyou821 as successive backcross parent) was displayed by cDNA-AFLP and differential transcripts-derived fragments (TDFs). The full-length cDNA of 1,707 bp with 1,323 bp open reading frame (ORF, open box), named Rsk was identified by RT-RACE from the resistance related fragment 40-2 in Zhongyou821. It encoded a protein of 440 amino acid residues, which contained a putative extracellular domain, a transmembrane domain, and an intracellular domain. Analysis of the deduced amino acid sequence of Rsk revealed that it had high homology to Arabidopsis thaliana kinase involved in protein binding, and had a conserved region of LRR-RI, indicating it might be a member of leucine-rich repeats, ribonuclease inhibitor-like subfamily. DNA sequences of 1,685 bp and 1,703 bp without an intron were also identified from Zhongyou821 and BC(5,) respectively. Expression analysis showed that Rsk might play a role in disease resistance pathways.
语种: 英语
WOS记录号: WOS:000291600200023
ISSN号: 0137-5881
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16379
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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Recommended Citation:
Zhang, Yu; Hu, Chun-xiang; Zhang, Cheng-gui; Gan, Li.Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum,ACTA PHYSIOLOGIAE PLANTARUM,2011,33(4):1277-1283
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