IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum
Zhang, Yu; Hu, Chun-xiang; Zhang, Cheng-gui; Gan, Li; Gan, L (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China; ganli@mail.hzau.edu.cn
2011
Source PublicationACTA PHYSIOLOGIAE PLANTARUM
ISSN0137-5881
Volume33Issue:4Pages:1277-1283
AbstractBased on the significant differences in the resistance to Sclerotinia sclerotiorum between two Brassica napus cultivars, ZhongR888 and Zhongyou821, near isogenic lines (NILs) were established. The resistance differences between Zhongyou821 and BC(5) progeny (the fifth generation from a cross between Zhongyou821 and ZhongR888 with Zhongyou821 as successive backcross parent) was displayed by cDNA-AFLP and differential transcripts-derived fragments (TDFs). The full-length cDNA of 1,707 bp with 1,323 bp open reading frame (ORF, open box), named Rsk was identified by RT-RACE from the resistance related fragment 40-2 in Zhongyou821. It encoded a protein of 440 amino acid residues, which contained a putative extracellular domain, a transmembrane domain, and an intracellular domain. Analysis of the deduced amino acid sequence of Rsk revealed that it had high homology to Arabidopsis thaliana kinase involved in protein binding, and had a conserved region of LRR-RI, indicating it might be a member of leucine-rich repeats, ribonuclease inhibitor-like subfamily. DNA sequences of 1,685 bp and 1,703 bp without an intron were also identified from Zhongyou821 and BC(5,) respectively. Expression analysis showed that Rsk might play a role in disease resistance pathways.
KeywordBrassica Napus Cdna-aflp Lrr-ri Northern Blotting Rt-race Sclerotinia Sclerotiorum Semiquantitative Rt-pcr
Department[Gan, L] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China; [Zhang, Y; Hu, CX] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhang, Y] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Zhang, CG] Dali Univ, Coll Pharm, Dali 671003, Peoples R China
Subject AreaPlant Sciences
Funding OrganizationNational Nature Science Foundation of China[30170600]; Hubei Nature Science Foundation[2001ABD113] ; National Nature Science Foundation of China[30170600]; Hubei Nature Science Foundation[2001ABD113] ; National Nature Science Foundation of China[30170600]; Hubei Nature Science Foundation[2001ABD113] ; National Nature Science Foundation of China[30170600]; Hubei Nature Science Foundation[2001ABD113]
Indexed BySCI
Language英语
WOS IDWOS:000291600200023
Funding OrganizationNational Nature Science Foundation of China[30170600]; Hubei Nature Science Foundation[2001ABD113] ; National Nature Science Foundation of China[30170600]; Hubei Nature Science Foundation[2001ABD113] ; National Nature Science Foundation of China[30170600]; Hubei Nature Science Foundation[2001ABD113] ; National Nature Science Foundation of China[30170600]; Hubei Nature Science Foundation[2001ABD113]
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16379
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorGan, L (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China; ganli@mail.hzau.edu.cn
Recommended Citation
GB/T 7714
Zhang, Yu,Hu, Chun-xiang,Zhang, Cheng-gui,et al. Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum[J]. ACTA PHYSIOLOGIAE PLANTARUM,2011,33(4):1277-1283.
APA Zhang, Yu,Hu, Chun-xiang,Zhang, Cheng-gui,Gan, Li,Gan, L ,&ganli@mail.hzau.edu.cn.(2011).Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum.ACTA PHYSIOLOGIAE PLANTARUM,33(4),1277-1283.
MLA Zhang, Yu,et al."Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum".ACTA PHYSIOLOGIAE PLANTARUM 33.4(2011):1277-1283.
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