IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris
Liu, Xiaoxiang; Wang, Yali; Gao, Hong; Xu, Xudong; Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; xux@ihb.ac.cn
2011
Source PublicationGENE
ISSN0378-1119
Volume482Issue:40545Pages:51-58
AbstractAn Antarctic strain (NJ-7) of Chlorella vulgaris possesses the same 18S rRNA sequence as that of a temperate strain (UTEX259), but shows significantly higher freezing tolerance than the latter. Suppression subtractive hybridization (SSH) was performed to identify genes of intensified expression in NJ-7 relative to UTEX259. Among the genes identified, Ccor1 and Ccor2, co-organized in the same gene cluster Ccor1-Ccor2-Ccor1-Ccor2, showed much higher expression levels in NJ-7 than in UTEX259 at both 20 degrees C and 4 degrees C. As detected by Northern blot and Western blot analyses, the two genes were cold-inducible in NJ-7 but almost not expressed in UTEX259. Their encoded products are predicted to share 55.7% identity to each other and possess physicochemical characteristics similar to that of late embryogenesis abundant (LEA) proteins in plants. The purified recombinant Ccor1 and Ccor2 showed high heat-stability and could act as cryoprotectants to lactate dehydrogenase in vitro. Based on their expression patterns and protein characteristics, we propose that Ccor1 and Ccor2 are two novel LEA proteins and are related to the greatly enhanced freezing tolerance in the Antarctic strain. (C) 2011 Elsevier B.V. All rights reserved.
KeywordChlorella Vulgaris Antarctic Strain Freeze Tolerance Late Embryogenesis Abundant Proteins Cryoprotective Activity
Department[Liu, XX; Wang, YL; Gao, H; Xu, XD] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Subject AreaGenetics & Heredity
Funding OrganizationChinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332]
Indexed BySCI
Language英语
WOS IDWOS:000293496300007
Funding OrganizationChinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16369
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; xux@ihb.ac.cn
Recommended Citation
GB/T 7714
Liu, Xiaoxiang,Wang, Yali,Gao, Hong,et al. Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris[J]. GENE,2011,482(40545):51-58.
APA Liu, Xiaoxiang,Wang, Yali,Gao, Hong,Xu, Xudong,Xu, XD ,&xux@ihb.ac.cn.(2011).Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris.GENE,482(40545),51-58.
MLA Liu, Xiaoxiang,et al."Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris".GENE 482.40545(2011):51-58.
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