中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
学科主题: Genetics & Heredity
题名: Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris
作者: Liu, Xiaoxiang ; Wang, Yali ; Gao, Hong ; Xu, Xudong
通讯作者: Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China ; xux@ihb.ac.cn
关键词: Chlorella vulgaris ; Antarctic strain ; Freeze tolerance ; Late embryogenesis abundant proteins ; Cryoprotective activity
刊名: GENE
发表日期: 2011
卷: 482, 期:40545, 页:51-58
收录类别: SCI
部门归属: [Liu, XX; Wang, YL; Gao, H; Xu, XD] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
资助者: Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332]
摘要: An Antarctic strain (NJ-7) of Chlorella vulgaris possesses the same 18S rRNA sequence as that of a temperate strain (UTEX259), but shows significantly higher freezing tolerance than the latter. Suppression subtractive hybridization (SSH) was performed to identify genes of intensified expression in NJ-7 relative to UTEX259. Among the genes identified, Ccor1 and Ccor2, co-organized in the same gene cluster Ccor1-Ccor2-Ccor1-Ccor2, showed much higher expression levels in NJ-7 than in UTEX259 at both 20 degrees C and 4 degrees C. As detected by Northern blot and Western blot analyses, the two genes were cold-inducible in NJ-7 but almost not expressed in UTEX259. Their encoded products are predicted to share 55.7% identity to each other and possess physicochemical characteristics similar to that of late embryogenesis abundant (LEA) proteins in plants. The purified recombinant Ccor1 and Ccor2 showed high heat-stability and could act as cryoprotectants to lactate dehydrogenase in vitro. Based on their expression patterns and protein characteristics, we propose that Ccor1 and Ccor2 are two novel LEA proteins and are related to the greatly enhanced freezing tolerance in the Antarctic strain. (C) 2011 Elsevier B.V. All rights reserved.
语种: 英语
WOS记录号: WOS:000293496300007
ISSN号: 0378-1119
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16369
Appears in Collections:藻类生物学及应用研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris.pdf(757KB)----开放获取--View Download

Recommended Citation:
Liu, Xiaoxiang; Wang, Yali; Gao, Hong; Xu, Xudong.Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris,GENE,2011,482(40545):51-58
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Xiaoxiang]'s Articles
[Wang, Yali]'s Articles
[Gao, Hong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Xiaoxiang]‘s Articles
[Wang, Yali]‘s Articles
[Gao, Hong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Identification and characterization of genes encoding two novel LEA proteins in Antarctic and temperate strains of Chlorella vulgaris.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace