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Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China
Jiang, Yongguang; Shao, Jihai; Wu, Xingqiang; Xu, Yao; Li, Renhui; Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; reli@ihb.ac.cn
2011
Source PublicationFEMS MICROBIOLOGY LETTERS
ISSN0378-1097
Volume322Issue:2Pages:108-114
AbstractThe microcystin-degrading genes, mlr, are important participants in the degradation process of hepatotoxic microcystins for several bacterial species. However, their expression status during degrading microcystins is still unknown. In order to study this expression process, we isolated a novel microcystin-degrading bacterial strain, sequenced its mlr gene cluster and examined the expression of the mlrA gene at different concentrations of microcystin LR. The expression of mlrA increased slightly at 0.4 mg L(-1), and was significantly upregulated at 2.0 mg L(-1). Frameshift mutations were found in the mlrB* gene, and the mRNA of mlrB* could not be detected in the total RNA extracts of Novosphingobium sp. THN1. We conclude that mlrA is actively involved in the microcystin-degrading process, but mlrB* has lost its activity in this bacterial strain.
KeywordNovosphingobium Degradation Efficiency Gene Expression Frame-shift Mutation
Department[Jiang, YG; Li, RH] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Jiang, YG] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Shao, JH] Hunan Agr Univ, Resources & Environm Coll, Changsha, Hunan, Peoples R China; [Wu, XQ] Chinese Acad Sci, NE Inst Geog & Agr Ecol, Changchun, Peoples R China; [Xu, Y] Nanjing Normal Univ, Coll Geog Sci, Nanjing, Peoples R China
Subject AreaMicrobiology
Funding OrganizationState Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501]
Indexed BySCI
Language英语
WOS IDWOS:000293793200002
Funding OrganizationState Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501]
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Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16367
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorLi, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; reli@ihb.ac.cn
Recommended Citation
GB/T 7714
Jiang, Yongguang,Shao, Jihai,Wu, Xingqiang,et al. Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China[J]. FEMS MICROBIOLOGY LETTERS,2011,322(2):108-114.
APA Jiang, Yongguang.,Shao, Jihai.,Wu, Xingqiang.,Xu, Yao.,Li, Renhui.,...&reli@ihb.ac.cn.(2011).Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China.FEMS MICROBIOLOGY LETTERS,322(2),108-114.
MLA Jiang, Yongguang,et al."Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China".FEMS MICROBIOLOGY LETTERS 322.2(2011):108-114.
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